In vitro antischistosomal activity of Artemisia annua and Artemisia afra extracts

Taljaard, Lorencia; Probst, Alexandra; Tornow, Robert; Keiser, Jennifer; Haynes, Richard K.; Kooy, Frank van der

doi:10.1016/j.phyplu.2022.100279

Cited by 11 publications

(19 citation statements)

References 20 publications

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“…62 This suggests the natural occurrence of dihydro-ART in the Artemisia genus, an observation that was confirmed recently. 96 The sesquiterpene content of A. afra infusions was never investigated except for the absence or extremely low amount of ART, 14,24,29,30 this plant species seeming also deprived of the infusion-soluble sesquiterpenes found in A. annua ( e.g. , arteannuin B and dihydroartemisinic acid).…”

Section: Infusion-specific Natural Products From Artemisia Annua and ...mentioning

confidence: 99%

“…26,61,62,94,115 A. afra infusion specifically contains the aglycone quercetin although its presence seems cultivar-dependent. 30,63 This suggests that other flavonoid aglycones present in A. afra ( e.g. , kaempferol and tamarixetin, being close in structure to quercetin), as well as water-soluble heterosides other than cynaroside, might also be detected in its infusion upon deeper analysis (Table 1).…”

Section: Infusion-specific Natural Products From Artemisia Annua and ...mentioning

confidence: 99%

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Future antimalarials fromArtemisia? A rationale for natural product mining against drug-refractoryPlasmodiumstages

2023

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Section: Infusion-specific Natural Products From Artemisia Annua and ...mentioning

confidence: 99%

Section: Infusion-specific Natural Products From Artemisia Annua and ...mentioning

confidence: 99%

Future antimalarials fromArtemisia? A rationale for natural product mining against drug-refractoryPlasmodiumstages

2023

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“…6 7 It is currently used for the treatment of flu-like symptoms, and extensively for COVID-19. 8 Infusions prepared from A. afra have shown interesting in vitro activity against HIV, schistosomiasis, Plasmodium spp., and lung cancer cell lines [9][10][11][12] However, Liu et al 13 did not find any in vitro antiplasmodial activity for the A. afra tea infusion. Potentially, the chemical variation of the individual markers present in A. afra can directly influence the bioactivity, especially since A. afra is used for the prevention of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).…”

Section: Introductionmentioning

confidence: 98%

“…Infusions prepared from A. afra have shown interesting in vitro activity against HIV, schistosomiasis, Plasmodium spp., and lung cancer cell lines 9–12 However, Liu et al 13 . did not find any in vitro antiplasmodial activity for the A. afra tea infusion.…”

Section: Introductionmentioning

confidence: 98%

Geographical and seasonal phytochemical variation of Artemisia afra Jacq. ex Willd

Olivier

Kooy

Gerber

2022

Phytochemical Analysis

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Introduction: Artemisia afra Jacq. ex. Willd. (Asteraceae) is a popular traditional medicine in South Africa, mainly used in the form of an infusion, for the treatment of respiratory ailments. Quality control methods are limited and phytochemical variation for the infusion is not well known.Objective: To develop a sensitive quality control method for A. afra infusions by validating a liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS) method and quantitatively comparing six marker compounds in A. afra samples collected from different locations and over a 12-month period.Material and methods: There was a multiple reaction monitoring method optimised and validated, according to ICH and FDA guidelines, to quantify the chemical markers present in infusions. Results:The chemistry differed significantly and interestingly, with an interchangeable trend between chlorogenic acid (CGA) and 4,5-dicaffeoylquinic acid (DCQA) observed in the samples collected monthly, elevated levels of CGA during winter and elevated levels of DCQA during summer. The remaining four markers showed a steady decrease as winter approached and a steady increase as summer approached.The ranges of the six markers were the following: CGA (0.68-14.68 μg/mg), DCQA (0.005-8.110 μg/mg), quercetin (0.01-0.65 μg/mg), luteolin (0.05-1.30 ng/mg), scopoletin (0.10-1.14 μg/mg), scopolin (0.03-1.21 μg/mg).Conclusions: A sensitive LC-ESI-MS/MS method was developed, validated, and used to quantify six marker compounds. The results indicated a large degree of phytochemical variation occurred across all samples tested, which highlights the importance of producing herbal medicine under controlled conditions and the necessity of analytical quality control methods.

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“…In this regard, several in vitro studies have already been performed to search for new active compounds from medicinal plants against different stages of S. mansoni, and promising results have been reported [15][16][17][18][19][20][21][22][23]. Pedilanthus tithymaloides (Linn) Poit., also known as Euphorbia tithymaloides, is a common succulent shrub native to tropical and subtropical America belonging to the family Euphorbiaceae.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Cercaricidal and Schistosomicidal Activities of the Raffia Wine and Hydroethanolic Extracts of Pedilanthus tithymaloides Linn (Poit). Stem Barks

Nkondo

Jatsa

Feussom

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Schistosomiasis control remains a public health concern, and there is a need to evaluate new strategies for targeting larval and adult stages of the parasite. As Pedilanthus tithymaloides is empirically used to treat schistosomiasis, it becomes essential to know its e ective action scienti cally. is study assessed the cercaricidal and schistosomicidal activity of P. tithymaloides stem barks ra a wine extract (RwPt) and hydroethanolic extract (HePt). Di erent concentrations of these extracts were tested against cercariae (31.25-1000 μg/mL) and adult worms (62.5-2000 μg/mL) of Schistosoma mansoni. Niclosamide-olamine 5% (1 μg/mL) and praziquantel (10 μg/mL) were used as pharmacological controls. Cercariae viability was determined every 30 min for 180 min, and adult worms' motor activity and viability after 24 and 48 h incubation. In addition, cytotoxicity and phytochemical analysis were performed. HePt was lethal to cercariae and adult worms with LC 50 of 73.91 μg/mL after 60 min of incubation and 731.17 μg/ mL after 48 h of incubation, respectively. Furthermore, a signi cant reduction of 94.44% in motor activity was observed in surviving worms at the concentration of 2000 μg/mL. RwPt was less e ective on S. mansoni cercariae with an LC 50 of 617.86 μg/mL after 180 min and on adult worms with a mortality rate of 9.83% at 2000 μg/mL for 48 h incubation. Both extracts showed a weak cytotoxicity pro le with an IC 50 of 983.50 μg/mL for HePt and more than 1000 μg/mL for RwPt. e LC-MS analysis of HePt allowed the detection of two annotated diterpenoids. Based on the selectivity index, the hydroethanolic extract of P. tithymaloides stem barks disclosed an intense cercaricidal activity and a moderate schistosomicidal e ect with low cytotoxicity. ese ndings may support the potential use of Pedilanthus tithymaloides as a natural product or a source of natural-derived compounds for interrupting schistosomiasis transmission.

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In vitro antischistosomal activity of Artemisia annua and Artemisia afra extracts

Cited by 11 publications

References 20 publications

Future antimalarials fromArtemisia? A rationale for natural product mining against drug-refractoryPlasmodiumstages

Future antimalarials fromArtemisia? A rationale for natural product mining against drug-refractoryPlasmodiumstages

Geographical and seasonal phytochemical variation of Artemisia afra Jacq. ex Willd

In Vitro Cercaricidal and Schistosomicidal Activities of the Raffia Wine and Hydroethanolic Extracts of Pedilanthus tithymaloides Linn (Poit). Stem Barks

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