In vitro antiproliferative and antioxidant activity of three fungal strains from the White sea

Couttolenc, Alan; Padrón, José M.; Shnyreva, A.V.; Sergeeva, Albina I.; Кураков, А. В.; Trigos, Ángel

doi:10.1016/j.polar.2021.100724

Cited by 2 publications

(1 citation statement)

References 29 publications

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“…As part of our interest in the bioprospection of microorganisms [ 37 , 38 , 39 ] and native plants [ 40 , 41 , 42 , 43 , 44 ], we tested the antiproliferative activity of the n -hexane extract of G. pinifolium against a panel of six representative human solid tumor cell lines: A549 and SW1573 (lung), HBL-100 and T-47D (breast), HeLa (cervix) and WiDr (colon). The GI 50 values showed growth inhibition in all cell lines in the range of 7.6–20 µg/mL.…”

Section: Resultsmentioning

confidence: 99%

Phenolic Fingerprinting and Bioactivity Profiling of Extracts and Isolated Compounds from Gypothamnium pinifolium Phil.

et al. 2022

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Gypothamnium pinifolium Phil. (Asteraceae) is a small shrub that grows in the Paposo Valley of the II Antofagasta Region of Chile. This initial study is of the high-resolution phenolic fingerprinting, antioxidant activity, the relaxation effects in rat aorta, the inhibitory enzyme potential, plus the antiproliferative activity of the ethyl acetate and n-hexane extract from G. pinifolium and its two major isolated secondary metabolites (one coumarin: 2-nor-1,2-secolycoserone, and one diterpene: ent-labda-8,13-E-diene-15-ol). The study involves using ultra-high-performance liquid chromatography todiode array detection coupled with Q-Orbitrap mass spectrometry analysis (UHPLC-PDA-Orbi-trap-MS), in which various compounds were identified, including specific coumarins. The n-hexane extract showed total phenolic and flavonoid contents of 517.4 ± 12.5 mg GAE/100 g extract and 72.3 ± 3.7 mg QE/100 g extract, respectively. In addition, the antioxidant activity of the n-hexane extract was assessed using in-vitro assays such as bleaching of DPPH and ABTS (IC50: 14.3 ± 0.52 and 2.51 ± 0.43 µg extract/mL, respectively), FRAP (347.12 ± 1.15 μmol Trolox equivalent/g extract), and ORAC (287.3 ± 1.54 μmol Trolox equivalents/g extract). Furthermore, the inhibition against cholinesterases (acetylcholinesterase (AChE) 4.58 ± 0.04 µg/mL, butyrylcholinesterase (BChE) IC50: 23.44 ± 0.03 µg/mL) and tyrosinase (IC50: 9.25 ± 0.15 µg/mL) enzymes of the n-hexane extract, and main compounds (IC50: 1.21 ± 0.03 µg/mL, 11.23 ± 0.02 µg/mL, 3.23 ± 0.12 µg/mL, and 103.43 ± 16.86 µg/mL, correspondingly for the most active coumarin 1) were measured. The antiproliferative potential of the extracts and the two principal compounds against several solid human cancer cells was investigated. All of them showed good activity against cancer cells. Label-free live-cell imaging studies on HeLa cells exposed to the isolated coumarin and the diterpene enabled the observation of cell death and several apoptotic hallmarks. Our results indicate that G. pinifolium Phil. is a valuable source of secondary metabolites with potential activity against noncommunicable diseases.

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Section: Resultsmentioning

confidence: 99%

Phenolic Fingerprinting and Bioactivity Profiling of Extracts and Isolated Compounds from Gypothamnium pinifolium Phil.

et al. 2022

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Antiproliferative and Antibacterial Activity of Polyporoid Fungi from Veracruz, Mexico

González-Solís,

Mendoza,

Ramos

et al. 2024

Int J Med Mushrooms

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Polyporoid fungi represent a vast source of bioactive compounds with potential pharmacological applications. The importance of polyporoid fungi in traditional Chinese medicine has led to an extensive use of some species of Ganoderma for promoting health and longevity because their consumption is associated with several bioactivities. Nevertheless, bioactivity of some other members of the Polyporaceae family has also been reported. This work reports the antiproliferative and antibacterial activity of crude extracts obtained from fruiting bodies of polypore fungi collected from the central region of Veracruz, Mexico, aimed at understanding the diversity of polypore species with potential pharmacological applications. 29 collections were identified macro- and microscopically in 19 species of polyporoid fungi, belonging to 13 genera. The antiproliferative activity screening of extracts against solid tumor cell lines (A549, SW1573, HeLa, HBL-100, T-47D, WiDr) allow us to identify four extracts with strong bioactivity [half-maximal growth inhibition (GI50) ≤ 50 μg/mL]. After this, a phylogenetic analysis of DNA sequences from the ITS region obtained from bioactive specimens allowed us to identify three extracts as Pycnoporus sanguineus (GI50 = ≤ 10 μg/mL) and the fourth bioactive extract as Ganoderma oerstedii (GI50 = < 50 μg/mL. Likewise, extracts from P. sanguineus showed mild or moderate antibacterial activity against Escherichia coli, Staphylococcus aureus and Xanthomonas albilineas. Bioprospecting studies of polyporoid fungi add to the knowledge of the diversity of macrofungi in Mexico and allow us to select one of the bioactive P. sanguineus to continue the pursuit of bioactive compounds through mycochemical studies.

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In vitro antiproliferative and antioxidant activity of three fungal strains from the White sea

Cited by 2 publications

References 29 publications

Phenolic Fingerprinting and Bioactivity Profiling of Extracts and Isolated Compounds from Gypothamnium pinifolium Phil.

Phenolic Fingerprinting and Bioactivity Profiling of Extracts and Isolated Compounds from Gypothamnium pinifolium Phil.

Antiproliferative and Antibacterial Activity of Polyporoid Fungi from Veracruz, Mexico

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