In vitro antiplasmodial effect of ethanolic extracts of traditional medicinal plant Ocimum species against Plasmodium falciparum

Inbaneson, Samuel Jacob; Sundaram, Ramalingam; Suganthi, Palavesam

doi:10.1016/s1995-7645(12)60004-2

Cited by 40 publications

(27 citation statements)

References 24 publications

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“…1). The IC 50 value (21.14 µg mLG 1 ) of the stem/root aqueous extract was found to be lower, thus was more active against P. falciparum relative to the root (IC 50 >100 µg mLG 1 ) and stem (IC 50 = 83.53 µg mLG 1 ) separately as reported by Inbaneson et al (2012). This could be due to the combined effects of the phytochemicals present in the roots and those in the stem.…”

Section: Resultsmentioning

confidence: 63%

“…The aqueous extract of the leaves with fruits of P. amarus gave an IC 50 value of 13.96 µg mLG 1 . The anti-plasmodial activity of the leaves of P. amarus has been reported by Inbaneson et al (2012) against P. falciparum. Their results indicated that the ethanolic leaf extract showed an IC 50 of 94.19 µg mLG 1 .…”

Section: Resultsmentioning

confidence: 99%

“…These IC 50 values were calculated by plotting the inhibition (%) against the logarithm to the base 10 of the concentrations of the extracts (log 10 concentration) using Microsoft Excel. The antiplasmodial activity was analyzed as described by Inbaneson et al (2012 …”

Section: Discussionmentioning

confidence: 99%

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In vitro Anti-Plasmodial Activity of Aqueous and Ethanolic Extracts of Moringa oleifera and Phyllanthus amarus

Donkor¹,

Oduro-Mensah²,

Ani³

et al. 2015

International J. of Biological Chemistry

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Many herbal medicinal products contribute to the treatment of malaria in endemic areas. In Ghana, there is documented evidence of the use of several plant species in the management of both infectious and non-infectious diseases. This study sought to validate the activity of extracts from two such plants, Phyllanthus amarus and Moringa oleifera, against Plasmodium falciparum. Anti-plasmodial activities of aqueous and ethanolic extracts of M. oleifera (whole plant and twig) and P. amarus (whole plant and stem) were assessed against the 3D7 laboratory strain of P. falciparum. Extracts were evaluated in vitro at concentrations of 12.5, 25, 50, 100 µg mLG 1 and the level of potency in each case was expressed as the concentration of the extract that inhibited 50% of the parasites (IC 50 ) relative to negative controls (100% parasitaemia). Artesunate was used as a positive standard in all assays. All extracts showed significant inhibition of parasite growth, with (IC 0 ) less than 50 µg mLG 1 , except the aqueous extract of the whole plant of P. amarus which showed a relatively high IC 50 of 115.43 µg mLG 1 . Interestingly, increasing concentration of ethanolic extract of combined twig and leaves of Moringa oleifera reduced inhibition of the parasite growth while a decreasing extract concentration resulted in increased parasite inhibition. The extracts of M. oleifera and P. amarus demonstrated potential anti-plasmodial activity which can be explored in malaria therapy. It is of interest to identify, isolate and characterize the active anti-plasmodial bioactive compounds from the plants.

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Section: Resultsmentioning

confidence: 63%

Section: Resultsmentioning

confidence: 99%

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In vitro Anti-Plasmodial Activity of Aqueous and Ethanolic Extracts of Moringa oleifera and Phyllanthus amarus

Donkor¹,

Oduro-Mensah²,

Ani³

et al. 2015

International J. of Biological Chemistry

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“…Kultur P. falciparum menggunakan lempeng sumur 24, masing-masing sumur berisi 1 mL kultur yang telah disinkronisasi menggunakan sorbitol 5% dengan parasitemia ± 1% dan hematokrit 5% dalam medium RPHS. Medium RPHS kemudian diganti dengan medium RPHS yang mengandung ekstrak air kayu akway berbagai konsentrasi (1 sampai 1x10 -7 mg/mL) (Inbaneson et al, 2012;Fidock et al, 2004).…”

Section: Metode Penelitianunclassified

PENGARUH PEMBERIAN EKSTRAK AIR KAYU AKWAY (Drymis piperita Hook. f.) PADA PERTUMBUHAN Plasmodium falciparum PENYEBAB MALARIA

Hermanto

Faramayuda

2017

kjif

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ABSTRAKIndonesia merupakan salah satu daerah endemik malaria. Resistensi obat malaria menjadi salah satu masalah dalam pengobatan malaria, oleh karen itu diperlukan pencarian obat malaria baru. Kulit kayu akway (Drimys piperita Hook. f) secara empiris digunakan oleh Suku Sogb Manokwari Papua sebagai antimalaria dan afrodisiaka. Penelitian ini diawali dengan proses pembuatan ekstrak air kulit kayu akway dengan metode perebusan menggunakan pelarut air suling. Pengujian aktivitas antimalaria menggunakan Plasmodium falciparum 3D7 secara in vitro. Ekstrak air kulit kayu akway menunjukan aktivitas antimalaria yang baik dengan nilai IC 50 sebesar 0,013 µg/mL.

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“…The main chemical constituents of O. sanctum are oleanolic acid, ursolic acid, rosmarinic acid, eugenol, carvacrol, linalool, and β-caryophyllene. [34] The antiviral activity of eugenol has been reported. Ocimum extracts are used in ayurvedic remedies for common colds, headaches, stomach disorders, inflammation, heart disease, various forms of poisoning, and malaria.…”

Section: Introductionmentioning

confidence: 99%

Assessment of potency of PC-complexed Ocimum sanctum methanol extract in embryonated eggs against Influenza virus (H1N1)

Jadhav¹,

Lal²,

Kshirsagar

2014

Phcog Mag

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Background:Despite of new vaccines, the threat of influenza infection persists. In addition, availability, cost, duration of protection rendered and effectiveness of vaccines additional to the need of effective drug therapy makes influenza a challenge, which the globe faces. Traditionally used herbs and their decoctions are used for ages to cure symptoms similar to influenza. Tulsi or Ocimum sanctum is one of these major herbs used for influenza-like disease treatment. We attempted to explore a new methodology for assessing phosphatidyl choline (PC)-complexed O. sanctum methanol extract in embryonated vaccine quality eggs model.Materials and Methods:The PC-complexed O. sanctum methanol extract was prepared and standardized using High-Performance Liquid Chromatography (HPLC). (Data not provided here) Nine to 11 days embryonated eggs were inoculated with the virus and drug mixture and then harvested to perform a hemagglutination (HA) test on the allantoic fluid. The experiments were performed at three different concentrations of ursolic acid with various virus concentration and dose levels of drugs. The HA titer was calculated from all experiments and observed for any inhibition of virus.Results:In initial experiments, matrix method for drug and virus concentration was employed. It was observed that the drug exhibited some response for 3log EID50 (egg infective dose) in few samples at 1:2 HA titer, but no response was observed at 4log EID50. In subsequent experiment, all the virus titers from 7log EID50 to 2log EID50 demonstrated positive HA titer of 1:64. However, the drug failed to exhibit any significant inhibition at any level of demonstrable virus titer. At all the concentrations, O. sanctum extracts were found to be safe.Conclusion:The embryonated egg model may be utilized further to screen other drugs, which possess direct inhibitory properties like neuraminidase inhibition, and O. sanctum does not inhibit the influenza virus in this model at the given concentration.

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In vitro antiplasmodial effect of ethanolic extracts of traditional medicinal plant Ocimum species against Plasmodium falciparum

Abstract: The ethanolic leaf extracts of O. sanctum possess lead compounds for the development of antiplasmodial drugs.

Cited by 40 publications

References 24 publications

In vitro Anti-Plasmodial Activity of Aqueous and Ethanolic Extracts of Moringa oleifera and Phyllanthus amarus

In vitro Anti-Plasmodial Activity of Aqueous and Ethanolic Extracts of Moringa oleifera and Phyllanthus amarus

PENGARUH PEMBERIAN EKSTRAK AIR KAYU AKWAY (Drymis piperita Hook. f.) PADA PERTUMBUHAN Plasmodium falciparum PENYEBAB MALARIA

Assessment of potency of PC-complexed Ocimum sanctum methanol extract in embryonated eggs against Influenza virus (H1N1)

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