In Vitro Antiplasmodial and Cytotoxic Activities of Compounds from the Roots of Eriosema montanum Baker f. (Fabaceae)

Tomani, Jean Claude Didelot; Bonnet, Olivier; Nyirimigabo, Alain; Deschamps, William; Tchinda, Alembert T.; Jansen, Olivia; Ledoux, Allison; Mukazayire, Marie-Jeanne; Vanhamme, Luc; Frédérich, Michel; Muganga, Raymond; Souopgui, Jacob

doi:10.3390/molecules26092795

Cited by 11 publications

(5 citation statements)

References 30 publications

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“…It appears from this study that the ethanolic extract was more active than the aqueous extract. Our results are similar to those obtained by Tomani et al [28] with Eriosema montanum Baker f. roots on Plasmodium 3D7 strain which had obtained an IC 50 of 17:68 ± 4:030 μg/ml of ethanolic extract and different from that obtained by Esseh et al [29] which had an IC 50 of 91:08 ± 0:61 μg/ml of aqueous extract. This difference can be explained by the type of extract, and the strain of Plasmodium used in this study, as well as the geographical location of the plant, may have had an impact on the observed difference.…”

Section: Discussionsupporting

confidence: 87%

Antiplasmodial, Antioxidant, and Cytotoxic Activity of Bridelia micrantha a Cameroonian Medicinal Plant Used for the Treatment of Malaria

Kevin

Cédric

Nadia

et al. 2023

BioMed Research International

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Introduction. Resistance to common antimalarial drugs and persistence of the endemicity of malaria constitute a major public health problem in Cameroon. The aim of this study was to evaluate the in vitro antiplasmodial, antioxidant, and cytotoxic activities of aqueous and ethanol extracts of Bridelia micrantha used by Cameroonian traditional healers for the treatment of malaria. Methods. Aqueous and ethanolic stem bark extracts were prepared according to standard procedures. The SYBR Green method was used for antiplasmodial activity on strains of Plasmodium falciparum sensitive to chloroquine (3D7) and resistant (Dd2). In vitro antioxidant activities of B. micrantha were determined using the scavenging activity of 2,2 ′ -diphenyl-1-picrylhydrazyl, nitric oxide, ferric reducing power, and hydrogen peroxide as well as their cytotoxicity on RAW 264.7 macrophage cells and red blood cells (RBC). Results. The aqueous and ethanol extracts of Bridelia micrantha showed antiplasmodial activity on the 3D7 strain with I C 50 of 31.65 ± 0.79 μg/ml and 19.41 ± 2.93 μg/ml, respectively, as well as 37.64 ± 0.77 μg/ml and 36.22 ± 1.04 μg/ml for the Dd2 strain, respectively. The aqueous and ethanol extracts showed free radical scavenging properties. The IC 50 aqueous and ethanol extract was approximately 0.0001737 μg/ml, 42.92 μg/ml, 1197 μg/ml, 63.78 μg/ml and 4.617 μg/ml, 429.9 μg/ml, 511 μg/ml, and 69.32 μg/ml for DPPH, NO, H2O2, and FRAP, respectively, which were compared to ascorbic acid ( 8.610 e − 005 μg/ml, 2901 μg/ml, 3237 μg/ml, and 18.57 μg/ml). The aqueous and ethanol extracts of B. micrantha were found to be nontoxic with CC 50 values of 950 ± 6.6 μg/ml and 308.3 ± 45.4 μg/ml, respectively. Haemolysis test showed that the two extracts were not toxic. Conclusion. These results suggest that B. micrantha can serve as an antimalarial agent. However, further studies are needed to validate the use of B. micrantha as an antimalarial.

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Section: Discussionsupporting

confidence: 87%

Antiplasmodial, Antioxidant, and Cytotoxic Activity of Bridelia micrantha a Cameroonian Medicinal Plant Used for the Treatment of Malaria

Kevin

Cédric

Nadia

et al. 2023

BioMed Research International

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“…For instance, several phytochemical classes such as triterpenoids, alkaloids, coumarins, flavonoids, steroids have been reported to contribute to the antiplasmodial activity of extracts [ 37 , 50 , 49 ]. Furthermore, Afzelia africana belongs to the Fabaceae family which has been known to exhibit promising antiplasmodial activities, with several active compounds isolated from them [ 52 , 53 ].…”

Section: Resultsmentioning

confidence: 99%

Bioassay-Guided Fractionation, ESI-MS Scan, Phytochemical Screening, and Antiplasmodial Activity of Afzelia africana

Vigbedor

Osei-Owusu²,

Kwakye

et al. 2022

Biochemistry Research International

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Background. Afzelia africana is a plant species with reported numerous medicinal potentials and secondary metabolites. Various parts of the plant have been applied for the treatment of hernia, rheumatism, pain, lumbago, malaria, etc. The study seeks to evaluate the phytochemical constituents, antiplasmodial, and ESI-MS scan of bioassay-guided fractions from the methanol extract of the bark of the plant. Aims. The main aim of the study was to carry out bioassay-guided fractionation of the crude methanol extract of Afzelia africana in order to isolate fractions and to evaluate their antiplasmodial activities and ESI-MS fingerprints. Methods. The methods employed include column chromatographic fractionation, phytochemical screening, antiplasmodial activity (malaria SYBER green assay (MSF)), and ESI-MS profile (full ESI-MS scan). Results. The column chromatographic fractionation and phytochemical screening of the plant led to the separation of the following four fractions: 1 (flavonoids, phenolics, glycosides, terpenoids, and steroids), 2 (alkaloids, anthraquinones, flavonoids, phenolics, glycosides, terpenoids, and steroids), 3 (anthraquinones, flavonoids, phenolics, glycosides, terpenoids, and steroids), and 4 (alkaloids, flavonoids, phenolics, glycosides, terpenoids, and steroids). The antiplasmodial activities of the fractions were tested against the 3D7 strain of Plasmodium falciparum with reported stronger activities for 1 (IC50: 0.097 ± 0.034 μg/mL) and 3 (IC50: 1.43 ± 0.072 μg/mL), and weaker activities for 2 (IC50: >100 μg/mL) and 4 (IC50: 37.09 ± 6.14 μg/mL). The full ESI-MS fingerprint of fractions 1, 2, 3, and 4 revealed the presence of 14, 24, 34, and 37 major molecular ions or compounds in each fraction, respectively.

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“…To further demonstrate the synthetic value of our methodology, the dehydroxylation protocol has been applied for the synthesis of bioactive molecules and the late-stage modification of natural products ( Scheme 6 ). For example, flavanone is a natural plant flavonoid found to inhibit tumor cells in vitro [ 35 , 36 ]. The 3-hydroxyflavanone 1w could be easily transformed into flavanone under our standard reaction conditions.…”

Section: Discussionmentioning

confidence: 99%

Na2S-Mediated One-Pot Selective Deoxygenation of α-Hydroxyl Carbonyl Compounds including Natural Products

Yan

Zhang

et al. 2022

Molecules

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A practical method for the deoxygenation of α-hydroxyl carbonyl compounds under mild reaction conditions is reported here. The use of cheap and easy-to-handle Na2S·9H2O as the reductant in the presence of PPh3 and N-chlorosuccinimide (NCS) enables the selective dehydroxylation of α-hydroxyl carbonyl compounds, including ketones, esters, amides, imides and nitrile groups. The synthetic utility is demonstrated by the late-stage deoxygenation of bioactive molecule and complex natural products.

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In Vitro Antiplasmodial and Cytotoxic Activities of Compounds from the Roots of Eriosema montanum Baker f. (Fabaceae)

Cited by 11 publications

References 30 publications

Antiplasmodial, Antioxidant, and Cytotoxic Activity of Bridelia micrantha a Cameroonian Medicinal Plant Used for the Treatment of Malaria

Antiplasmodial, Antioxidant, and Cytotoxic Activity of Bridelia micrantha a Cameroonian Medicinal Plant Used for the Treatment of Malaria

Bioassay-Guided Fractionation, ESI-MS Scan, Phytochemical Screening, and Antiplasmodial Activity of Afzelia africana

Na2S-Mediated One-Pot Selective Deoxygenation of α-Hydroxyl Carbonyl Compounds including Natural Products

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