In vitro Antioxidant potential, Total Phenolic and Flavonoid contents in Justicia gendarussa leaf extracts

Abstract

Free radicals are triggered in human body by routine metabolic pathways which are highly reactive substance entangled in various physiological functions but excess production may impart with multitude diseases such as cancer, arthritis, inflammation and various neurodegenerative disorders. As a result, previous findings supported that various photochemical constituents present in natural plants exerts prominent role in defense mechanism against rate of excess free radicals’ production. The present study was carried out to investigate phytochemical composition, quantification studies and in vitro antioxidant potential of Justicia gendarussa (JG) various solvent extracts. JG belongs to the family Acanthaceace and used as traditional healers in various ailments. JG plant material was collected, dried, powdered and subjected for soxhlation to prepare ethanolic, ethyl acetate, aqueous and n-hexane extracts. Screening of Active constituents by various qualitative tests, estimation of total phenolic (TPC) and Total flavonoid content (TFC) and in vitro antioxidant potential of plant extracts were performed by DPPH, Lipid peroxidation, Nitric oxide and Superoxide radical scavenging methods. Present investigation reveals that, presence of flavonoids, phenols, amino acids, glycosides, cardiac glycosides, proteins, carbohydrates, alkaloids, steroids, terpenoids and tannins. TPC and TFC of ethanol extract were found to be 9.47 ± 0.0216 mg Gallic acid equivalent (GAE/g) and 97.6 ± 0.0342 mg Rutin equivalent/g (RUE/g). Among all the analyzed extracts ethanolic extract of JG possessed high radical neutralizing capacity and for DPPH (IC50 32µg/ml), Lipid Peroxidation (28 µg/ml), Nitric oxide (30 µg/ml) and superoxide anion radicals (39 µg/ml) respectively and found to be more effective as ascorbic acid (26 µg/ml) which was used as standard. Overall results of the study concluded that JG leaf extracts possess beneficial Phytoconstituents, high phenol and flavonoid contents and potential antioxidant activity, which could be a viable source of natural antioxidants for treating various degenerative disorders.

Optimization Extraction for Total Phenolic, Flavonoid Contents, and Antioxidant Activity with Different Solvents and UPLC-MS/MS Metabolite Profiling of Justicia gendarussa Burm.f

“…These results show that semi-polar ethanol extracts flavonoids from the leaves. Previous studies showed the best extraction of flavonoids fro m this plant using ethanol as a solvent (Kuber, 2021).…”

“…In this study, the antioxidant activity according to the DPPH method showed optima l results by extraction using a hexane solvent, while it is based on the FRAP method using a mixed solvent of ethanol and hexane. Previous studies using water, ethanol, ethyl acetate, and hexane solvents reported that the ethanolic extract of J. gendarussa had the best antioxidant activity (Kuber, 2021).…”

Phenolic and flavonoid content with agro morphological characters of 12 accessions of Justica gendarussa grew in Indonesia

“…The leaves of J. gendarussa contain phenolics and flavonoids (Kuber 2021;Marliani et al 2022). Phenolics and flavonoids have important biological roles, such as plant growth regulation, defense mechanisms against pathogens and predators, plant pigmentation, protection from UV rays, and nitrogen fixation mechanisms.…”