In-vitro Antioxidant and Cytotoxic Activity of Crude Extracts of Pleurotus highking, a Potential Oyster Mushroom

Haque, M. N.; Rana,; Aktar, Koly; Chouduri,; Islam, Soriful

doi:10.9734/bjpr/2016/30594

Cited by 2 publications

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“…These mushrooms offer an alternative functional food source which could play an important role in improving the nutritional status and prevent a number of common diseases in Bangladeshi people. Several studies has claimed that different species of mushrooms grown in Bangladesh are good source of nutritional components and minerals with marked anti-oxidant potential [6,16,[20][21][22][23][24][25][26][27][28][29][30]. Besides several pharmacological activities including antityrosinase, cytotoxic, anti-hyperglycemic and antimicrobial potential of Bangladeshi mushrooms have been scientifically demonstrated [28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the nutritional value and antioxidant activities of common Bangladeshi edible mushrooms

et al. 2020

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Background Mushrooms have been recognized both as medicine and nutritional food in many countries for thousands of years, owing to the presence of significant amounts of carbohydrates, proteins, lipids, fibers, and antioxidants. This study aims at evaluating the nutritional contents and antioxidant potentials of seven types of mushrooms cultivated in Bangladesh. Methods Proximate composition analysis of the collected mushrooms was performed to determine moisture content, pH, lipid, crude fibre, total ash, protein, carbohydrate content, and calorific value. Antioxidant potential of collected mushrooms were evaluated by mean of eight different methods including total flavonoid content, phenolic content, tannin content, total antioxidant activity, hydroxyl radical scavenging activity, DPPH assay and reducing power capacity. Results The results demonstrated that investigated mushrooms were found rich in proteins (20–45 g/100 g), carbohydrate (11–61 g/100 g in dry sample) and fibre (5–40 g/100 g). The ash content was found 6–10 g/100 g and glucose content 54–160 mg/100 g. However, all the mushrooms showed a lower content of lipid (1–4%). Results also revealed that the total antioxidant capacity of the extracts were found in the concentration range of 0.08–0.21 mg/mL, whereas the hydroxyl radical and DPPH radical scavenging activity were 0.88–1.40 and 0.05–0.63 mg/mL. Conclusion The findings of the current investigation proved that the studied Bangladeshi mushrooms are good source of nutritional and antioxidant components. Therefore, this study can help spreading awareness among Bangladeshi people regarding consumption of mushrooms as functional foods.

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Section: Introductionmentioning

confidence: 99%

Investigation of the nutritional value and antioxidant activities of common Bangladeshi edible mushrooms

et al. 2020

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“…They contain diverse biomolecules that are used for the treatment of various diseases including cancer [5]. Although a number of studies have already been conducted to explore the antibacterial and antifungal [6], anti-HIV [7], antitumoral [8], cytotoxic activity [9], antilipidemic [10], hyperglycemic and hypotensive [11], antiinflammatory [12], and immunomodulatory [13] properties of some species of oyster mushrooms, no reports have been published indicating the anticancer properties of the P. highking mushroom.…”

Section: Introductionmentioning

confidence: 99%

“…Chronic oxidative stress and inflammation are major risk factors for cancer development. Previous studies [9,12] reported that P. highking is a promising source of antioxidant and antiinflammatory agents. Hence, we hypothesized that, by supplying antioxidant and antiinflammatory metabolites, P. highking may reduce the risk of cancer.…”

Section: Introductionmentioning

confidence: 99%

Pleurotus highking Mushroom Induces Apoptosis by Altering the Balance of Proapoptotic and Antiapoptotic Genes in Breast Cancer Cells and Inhibits Tumor Sphere Formation

Haque

Islam

2019

Medicina

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Background and objectives: Mushrooms that have medicinal properties are part of many traditional diets. The aim of the present study was to use the human breast cancer cell line MCF-7 to investigate the anticancer activity of Pleurotus highking mushroom purified extract fraction-III (PEF-III) and to elucidate the possible mechanism of that activity. Materials and Methods: The effects of PEF-III on cell proliferation and viability were evaluated by a colony formation assay and an MTT assay, respectively. Cell morphological changes, annexin-V phycoerythrin and propidium iodide (PI) staining, DNA fragmentation, and caspase 3/7 activity assays were performed to determine the induction of apoptosis by PEF-III. The genes responsible for regulation of apoptosis were analyzed by means of Western blot analysis. In vitro tumor sphere formation assay was performed using a 3D sphere culture system. Results: PEF-III significantly reduced the proliferation and viability of MCF-7 cells. Cell shrinkage and rounding, and annexin-V phycoerythrin and PI staining followed by flow cytometry indicated that the cell death was due to apoptosis. Additionally, a laddering DNA pattern and increased levels of caspase-3/7 enzyme also corroborated the notion of apoptosis-mediated cell death. This incidence was further confirmed by upregulation of proapoptotic genes (p53 and its target gene, Bax) and downregulation of the expression of an antiapoptotic gene (Bcl-2). PEF-III also reduced the size and number of the tumor spheres in 3D culture conditions. Conclusions: The anticancer activity of PEF-III is due to induction of apoptosis by a shift in the balance of proapoptotic and antiapoptotic genes. Therefore, the findings of the present study may open a path to exploring potential drug candidates from the P. highking mushroom for combating breast cancer.

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In-vitro Antioxidant and Cytotoxic Activity of Crude Extracts of Pleurotus highking, a Potential Oyster Mushroom

Cited by 2 publications

References 0 publications

Investigation of the nutritional value and antioxidant activities of common Bangladeshi edible mushrooms

Investigation of the nutritional value and antioxidant activities of common Bangladeshi edible mushrooms

Pleurotus highking Mushroom Induces Apoptosis by Altering the Balance of Proapoptotic and Antiapoptotic Genes in Breast Cancer Cells and Inhibits Tumor Sphere Formation

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