In Vitro Antioxidant and Antiproliferative Activities of Methanolic Plant Part Extracts of Theobroma cacao

Baharum, Zainal; Akim, Abdah Md; Taufiq‐Yap, Yun Hin; Hamid, Roslida Abdul; Kasran, Rosmin

doi:10.3390/molecules191118317

Cited by 71 publications

(55 citation statements)

References 39 publications

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“…In this study, the EOs displayed the strongest antiproliferative activity was found to show moderate antioxidant activity (unpublished work) suggesting that antioxidant effects have moderate effects on the antiproliferative activity. This observation has been supported statistically by Baharum and his coworkers [21], who reported that the anti-cancer activities of Theobroma cacao plant extracts showed negative moderate correlation with their antioxidant activity. Stock solution of the EO was prepared by dissolving the EO in DMSO (final conc.…”

Section: Resultssupporting

confidence: 56%

Antiproliferative Activity of EO Extracted from Different Aromatic Plants on Different Cell Lines

Al-Zubairi¹,

Al-Mamary²,

Al-Ghasani³

et al. 2017

MOJT

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Essential Oils (EOs) are used in many products to be intended for human utilization. Despite their pharmacological applications in the folk and traditional medicine, studies on EO anti-proliferative properties are still limited. The aim of this study was to investigate the anti-proliferative properties of hydrodistilled EO from 16 aromatic plants grown in Yemen. The cytotoxicity of the EOs was determined against seven cell lines namely; HeLa, MCF7, MDA-MB 231, CEMss, WEHI-3B, 3T3 and CHO cell lines using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) assay. Lantana camara EO was the most cytotoxic extract against all tested cell lines with an IC50 value of ≤ 0.01 % (v/v), except the non-tumorous cell lines CHO with IC50 value of 0.025 % ± 0.005 % v/v. While Cinnamomum zeylanicum EO showed high cytotoxic activity against HeLa, MDA-MB 231, 3T3 and CHO cell lines with IC50 value of ≤ 0.010 % (v/v). EO from Ocimum basilicum and Mentha piperita were among the ones with no activity or IC50 value of > 0. 010 % (v/v). The results demonstrated the potential of the EO from aromatic plants from Yemen for possible source of cancer treatment. type apparatus. The oil layer was collected, however, in some cases the distillate aqueous layers were washed with ether to extract any dissolved oils in water. Then, the ether was separated by separatory funnel and evaporated on water bath at 40 °C and the residue EO was added to the first collected portion. The EO was dried over anhydrous sodium sulfate and the yield was calculated. Keyword: Cell cultures and maintenanceHuman cervical cancer cells (HeLa), human breast cancer cell lines (MCF-7), human mammary cancer cell lines-estrogen negative (MDA-MB-231), human colon carcinoma cell lines (HT-29), mouse fibroblast cell lines (3T3) and murine monomyelocytic leukemia cell lines (WEHI-3) were obtained from ATTC. While Chinese hamster ovary cell line (CHO) from ECACC and T4-lymphoplastoid cell lines (CEMss) from NIH (AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH: USA). Cell lines were grown in RPMI 1640 supplemented with 10% fetal calf serum, 1% penicillin-streptomycin and 1% amphotericin B. Flasks containing cell lines were incubated in a humidified incubator with 5% CO 2 , at 37 °C. Cultures were frequently examined under inverted microscope (Micros, Austria). Once cells reached 80% confluency, media was removed and the cells were washed 3 times with 7mL of PBS (Phosphate Buffer Saline). Two milliliters of trypsin was added to the adherent cells and were incubated for 5 minutes. The flask was tapped gently to detach cells from the wall of the flask to appear as single cells. Ten milliliters of RPMI 1640 with 10% FCS were added to the flask and the content of the flask was resuspended to allow cells to disperse. About 6mL of cell suspension was transferred into a 75cm3 flask. Ten milliliters of RPMI 1640 with 10% FCS were then added and incubated in CO 2 incubator at 37 °C. The cells were frequently examined under an inverted microsco...

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Section: Resultssupporting

confidence: 56%

Antiproliferative Activity of EO Extracted from Different Aromatic Plants on Different Cell Lines

Al-Zubairi¹,

Al-Mamary²,

Al-Ghasani³

et al. 2017

MOJT

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show abstract

“…The cytotoxicity classification was sorting based on United State National Cancer Institute (USNCI) plant screening program. IC 50 value of extract that is between 20 and 100 μ g/mL is considered moderately cytotoxic [11, 22, 23] while less than 20 μ g/mL is considered strongly cytotoxic [23, 24]. …”

Section: Discussionmentioning

confidence: 99%

“…The percentage of cell viability (%) was calculated using the formula:

\begin{matrix} T3 \\ Cell viability (%) \\ = \frac{mean OD of treated cell - mean OD of blank}{mean OD of untreated cell - mean OD of blank} \\ \times 100 \end{matrix}

The cytotoxic effects against 4T1 cells after 72 hours were recorded as IC 50 compared with untreated cells [10, 11]. The percentages of cell viability values against concentration of respective extracts were plotted in order to determine the IC 50 values of each extract.…”

Section: Methodsmentioning

confidence: 99%

Cytotoxicity and Apoptosis Induction of Ardisia crispa and Its Solvent Partitions against Mus musculus Mammary Carcinoma Cell Line (4T1)

Nordin

Kadir

Zakaria

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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This study was conducted to investigate the cytotoxicity and apoptosis effect of A. crispa extract and its solvent partition (ethyl acetate and aqueous extract) against Mus musculus mammary carcinoma cell line (4T1). The normal mouse fibroblast cell line (NIH3T3) was used as comparison for selective cytotoxicity properties. The cytotoxicity evaluation was assessed using MTT assay. AO/PI dual fluorescent staining assay and Annexin V-FITC were used for apoptosis analysis. Results showed that 80% methanol extract from leaves showed most promising antimammary cancer agent with IC50 value of 42.26 ± 1.82 μg/mL and selective index (SI) value of 10.22. Ethyl acetate was cytotoxic for both cancer and normal cell while aqueous extract exhibited poor cytotoxic effect. 4T1 cells labelled with AO/PI and Annexin V-FITC and treated with 80% methanol extract demonstrated that the extract induces apoptosis to 4T1 mammary cancer cells. In conclusion, 80% methanol extract of A. crispa was selectively cytotoxic towards 4T1 cells but less cytotoxic towards NIH3T3 cells and induced the cancerous cells into apoptotic stage as early as 6 hours.

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“…Furthermore, saponin is a natural, low-priced and renewable glycoside that occurs naturally in different plants (over 100 families) and is expected to have potent antioxidant and anticancer activity. 36 For these reasons, the stability experiments in the present study focused on the lycopene microemulsions that were prepared using saponin under optimum operational conditions.…”

Section: Droplet Size and Pdimentioning

confidence: 99%

Stability assessment of lycopene microemulsion prepared using tomato industrial waste against various processing conditions

Amiri-Rigi

Abbasi

2017

J Sci Food Agric

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The lycopene microemulsion showed satisfactory stability over a process where its monodispersity and nanosize could be of potential advantage to the food and related industries. Regarding the carcinogenicity of synthetic colourants, potential applications of the lycopene microemulsion include in soft drinks and minced meat, which would result in a better colour and well-documented health-promoting qualities. © 2017 Society of Chemical Industry.

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In Vitro Antioxidant and Antiproliferative Activities of Methanolic Plant Part Extracts of Theobroma cacao

Cited by 71 publications

References 39 publications

Antiproliferative Activity of EO Extracted from Different Aromatic Plants on Different Cell Lines

Antiproliferative Activity of EO Extracted from Different Aromatic Plants on Different Cell Lines

Cytotoxicity and Apoptosis Induction of Ardisia crispa and Its Solvent Partitions against Mus musculus Mammary Carcinoma Cell Line (4T1)

Stability assessment of lycopene microemulsion prepared using tomato industrial waste against various processing conditions

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