In Vitro Antioxidant and Antiproliferative Activities of Novel Orange Peel Extract and It's Fractions on Leukemia HL-60 Cells

Abstract

In the present work, novel orange peel was extracted with 100%EtOH (ethanol) and fractionated into four fractions namely F1, F2, F3, F4 which were eluted from paper chromatographs using 100%EtOH, 80%EtOH, 50%EtOH and pure water respectively. The crude extract and its four fractions were evaluated for their total polyphenol content (TPC), total flavonoid content (TFC) and radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. Their cytotoxic activity using WST assay and DNA damage by agarose gel electrophoresis were also evaluated in a human leukemia HL-60 cell line. The findings revealed that F4 had the highest TPC followed by crude extract, F2, F3 and F1. However, the crude extract had the highest TFC followed by F4, F3, F2, and F1. Depending on the values of EC 50 and trolox equivalent antioxidant capacity, F4 possessed the strongest antioxidant activity while F1 and F2 displayed weak antioxidant activity. Further, incubation HL-60 cells with extract/fractions for 24h caused an inhibition of cell viability in a concentrationdependent manner. F3 and F4 exhibited a high antiproliferative activity with a narrow range of IC 50 values (45.9 -48.9µg/ml). Crude extract exhibited the weakest antiproliferative activity with an IC 50 value of 314.89 µg/ml. Analysis of DNA fragmentation displayed DNA degradation in the form of a smear-type pattern upon agarose gel after incubation of HL-60 cells with F3 and F4 for 6 h. Overall, F3 and F4 appear to be good sources of phytochemicals with antioxidant and potential anticancer activities.

Citrus species: Modern functional food and nutraceutical-based product ingredient

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Total Phenolic and Flavonoid Contents and Antioxidant Activity of Ethanol Fraction of Picria Fel-Terrae (Lour.) Herbs

“…To determine the content of flavonoids, we prepared a calibration curve using quercetin as standard. The flavonoid content is expressed as quercetin equivalents (QE) in mg/gram of extract [2,26]. The equation to determine total flavonoid concentration:…”

Antitumor and Antioxidant Effects of Flavonoid Fraction of Citrus sinensis peel Extract

“…[14][15] significant antioxidant [24][25][26][27] antibacterial, 28 antiproliferative activity. 29 In the present study in vitro and in vivo antitumor studies of orange peel flavonoids were conducted. The results from in vitro studies concealed that flavonoids showed moderate cytotoxicity and acted specially on cancer cell culture (L929) compared to the normal cell culture (vero).…”