In vitro antioxidant and antimicrobial activity of carotenoid pigment extracted from Sporobolomyces sp. isolated from natural source

Abstract

Abstract:The aim of the present study was to isolate and study about the antioxidant and antibacterial activity of carotenoid pigment. Sporobolomyces sp. isolated from the phyllosphere surface of rice plant has found to produce carotenoid pigment. The present investigation was carried out for antioxidant assays viz., DPPH, iron reducing and metal chelating activity. A steady increase in the antioxidant activities was observed in the carotenoid pigment with raising the pigment concentration. In the present study, the maximum antioxidation characteristics of carotenoid by DPPH, iron reducing and metal chelating assays (75.04 %, 1.88 % and 59.32 %) were achieved by pigmentation of Sporobolomyces sp. at the concentration of 100 µg ml -1 . The antibacterial activity was studied on several organisms like Enterococcus sp., Staphylococcus aureus, Streptococcus faecalis, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa. Among the six pathogens, the pigment was found to be more effective against E. coli (2.9 cm) and S. aureus (2.6 cm). This study revealed that yeast carotenoid pigment was a potential source for its use in food and pharmaceutical applications.

Antimicrobial Activity of Pigments Extracted from Rhodotorula glutinis Against Some Bacteria and Fungi

“…Among bacteria, Gram-negative bacteria had less sensitively compared to Gram-positive types, which represents higher resistance of Gram-negative bacteria to PER ( Table 1). The similar finding were observed by Umadevi and Krishnaveni [18]; and Manimala and Murugesan [19] for carotenoid pigment extracted from Micrococcus luteus KF532949 and Sporobolomyces sp. isolated from natural source, respectively, which reported that Gram-negative bacteria had higher resistance to the carotenoid pigments.…”

Isolation of Pigmented Yeasts, Extraction of Pigment and Study of Antimicrobial Property of its Pigment

“…Microbial synthesis offers a promising method for production of carotenoids. This explains the increasing interest in production of microbial carotenoids as alternative for synthetic food colorants as well as for using it as antibacterial agent (Manimala et al, 2014).…”

“…Phaffia, Rhodotorula, Cryptococcus and Sporobolomyces, fungi like Blakeslea trispora, and algae such as Dunaliella and Haematococcus and bacteria such as Flavobacterium and Micrococcus are reported to produce carotenoids (Manimala et al, 2014). Pigmented yeasts like Rhodotorula and Rhodosporidium produce the major carotenoid pigments viz.…”

Production of food colourants by filamentous fungi

“…Pigments from other fungi such as Monodictys castaneae SVJM139 (Visalakchi and Muthumary, 2010); Sporobolomyces sp. (Manimala and Murugesan, 2014), Fusarium sp. (Geweely, 2011;Mani et al, 2015), Aspergillus sp.…”