In vitro antioxidant and anticataractogenic potential of silver nanoparticles biosynthesized using an ethanolic extract of Tabernaemontana divaricata leaves

Anbukkarasi, Muniyandi; Thomas, Philip A.; Sheu, Joen Rong; Geraldine, Pitchairaj

doi:10.1016/j.biopha.2017.04.079

Cited by 28 publications

(17 citation statements)

References 62 publications

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“…Anbukkarasi et al reported the successful green-synthesizing of silver NPs (AgNPs) using an ethanolic extract of the leaves of Tabernaemontana divaricata, intended for use in anticataractogenic potential [43].…”

Section: State Of the Art In The Formulation Of Ophthalmic Nanosystems With Antioxidantsmentioning

confidence: 99%

“…This review of published data showed that ocular nanosystems with antioxidants presented significant protective effects against multiple ocular pathologies, demonstrated in specific in vivo or ex-vivo animal models. The studies by Li et al [47], Bodoki et al [41], and Anbukkarasi et al [43] used the selenite-induced cataract model in rodents, obtaining a reduction of the disease progression under treatment with nano entrapped antioxidants like baicalin, lutein, or silver. In this model, the antioxidant molecules protected the lens against oxidative damage caused by a toxic substance-sodium selenite.…”

Section: Preclinical In Vivo and Ex-vivo Studies With Ophthalmic Nanosystems With Antioxidantsmentioning

confidence: 99%

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Ophthalmic Nanosystems with Antioxidants for the Prevention and Treatment of Eye Diseases

et al. 2020

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Oxidative stress may induce a series of pathophysiological modifications that are directly involved in the development of ophthalmic diseases like age-related cataract, macular degeneration or diabetic retinopathy, considered to be responsible for the majority of vision loss cases. Although various treatment options for eye diseases are available, multiple factors could limit their efficacy. Recently, the accelerated development of ophthalmic nanosystems has provided new possibilities for overcoming the limitations of existing ocular drug delivery methods. This review evaluates the current status of ophthalmic nanosystems loaded with antioxidants for the prevention and treatment of several eye diseases.

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Section: State Of the Art In The Formulation Of Ophthalmic Nanosystems With Antioxidantsmentioning

confidence: 99%

Section: Preclinical In Vivo and Ex-vivo Studies With Ophthalmic Nanosystems With Antioxidantsmentioning

confidence: 99%

Ophthalmic Nanosystems with Antioxidants for the Prevention and Treatment of Eye Diseases

et al. 2020

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“…The genus Tabernaemontana , one of the largest groups in the subfamily Rauvolfioideae, includes approximately 100 species distributed in different tropical regions worldwide. This genus is widely used in ethnobotanical medicine because of its various documented biological activities, such as antioxidant [ 21 ], anti-inflammatory [ 22 ], anticarcinogenic [ 23 ] and antimicrobial activity [ 24 ]. In particular, larvicidal activity against larval stages III and IV of Aedes aegypti [ 25 ] and antiviral activity against various strains of DENV-2 and cell lines [ 16 ] have been observed in ethanol extracts from the species Tabernaemontana cymosa Jacq ( T. cymosa ).…”

Section: Introductionmentioning

confidence: 99%

The Antiviral and Virucidal Activities of Voacangine and Structural Analogs Extracted from Tabernaemontana cymosa Depend on the Dengue Virus Strain

Monsalve-Escudero

Loaiza-Cano

Zapata-Cardona

et al. 2021

Plants

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Currently, no specific licensed antiviral exists for treating the illness caused by dengue virus (DENV). Therefore, the search for compounds of natural origin with antiviral activity is an important area of research. In the present study, three compounds were isolated and identified from seeds of Tabernaemontana cymosa plants. The in vitro antiviral effect of those compounds and voacangine against different DENV strains was assessed using different experimental approaches: compounds added before the infection (Pre), at the same time with the virus (Trans), after the infection (Post) or compounds present in all moments of the experiment (Pre-Trans-Post, Combined treatment). In silico studies (docking and molecular dynamics) were also performed to explain the possible antiviral mechanisms. The identified compounds were three structural analogs of voacangine (voacangine-7-hydroxyindolenine, rupicoline and 3-oxo-voacangine). In the Pre-treatment, only voacangine-7-hydroxyindolenine and rupicoline inhibited the infection caused by the DENV-2/NG strain (16.4% and 29.6% infection, respectively). In the Trans-treatment approach, voacangine, voacangine-7-hydroxyindolenine and rupicoline inhibited the infection in both DENV-2/NG (11.2%, 80.4% and 75.7% infection, respectively) and DENV-2/16681 infection models (73.7%, 74.0% and 75.3% infection, respectively). The latter strain was also inhibited by 3-oxo-voacangine (82.8% infection). Moreover, voacangine (most effective virucidal agent) was also effective against one strain of DENV-1 (DENV-1/WestPac/74) and against the third strain of DENV-2 (DENV-2/S16803) (48.5% and 32.4% infection, respectively). Conversely, no inhibition was observed in the post-treatment approach. The last approach (combined) showed that voacangine, voacangine-7-hydroxyindolenine and rupicoline inhibited over 90% of infections (3.5%, 6.9% and 3.5% infection, respectively) of both strains (DENV-2/NG and DENV-2/16681). The free energy of binding obtained with an in silico approach was favorable for the E protein and compounds, which ranged between −5.1 and −6.3 kcal/mol. Finally, the complex formed between DENV-2 E protein and the best virucidal compound was stable for 50 ns. Our results show that the antiviral effect of indole alkaloids derived from T. cymose depends on the serotype and the virus strain.

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“…9 Antioxidants from natural sources, rather than synthetic antioxidants, are preferred for use. 10 Antioxidant-replete plant extracts which reportedly exhibit anticataractogenic potential include Pleurotus ostreatus (Oyster mushroom), 11 Ocimum sanctum, 12 Vitex negundo, 13 Cineraria maritima, 14 Aegle marmelos, 15 Tabernaemontana divaricata 16 and Leucas aspera. 17 Crude extracts of medicinal herbs are possibly more biologicallyactive than are purified compounds.…”

Section: Introductionmentioning

confidence: 99%

A Methanolic Extract of Ocimum basilicum Exhibits Antioxidant Effects and Prevents Selenite-induced Cataract Formation in Cultured Lenses of Wistar Rats

Anand¹,

Sundararajan²,

Anbukkarasi³

et al. 2019

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Objective: An extract (Methanolic) of the Ocimum basilicum leaf was analysed for potential to abrogate experimental formation of cataract in-vitro. Methods: Phytoconstituents were first detected in O. basilicum extracts (Aqueous or methanolic) by gas chromatographic-mass spectrometric analysis. The putative antioxidant activity of these extracts was then assessed by measuring in-vitro radical-scavenging activity, ion-chelating potential and reducing potency. Potential cytotoxicity of the extract on Human lenticular epithelial B3 (HLE-B3) cells was also sought. Finally, possible prevention of cataract formation by the methanolic extract was gauged in selenite-exposed lenses obtained from Wistar rats. There were 3 groups (8 Lenses in each): Group I (Lenses incubated in Dulbecco's modified Eagle's medium [DMEM] alone); Group II (Lenses incubated in DMEM with sodium selenite [100 µM/ml]); Group III (Lenses incubated in DMEM with selenite [100 µM/ml] and the O. basilicum methanolic extract (200 µg/ml DMEM). Gross lenticular morphology was assessed. Levels of lenticular malondialdehyde (MDA) and reduced glutathione (GSH) were also measured. Results: A higher intensity of antioxidative activity was noted in the methanolic extract than in the aqueous extract. The methanolic extract exhibited negligible cytotoxicity. On morphological examination, marked opacification was seen in all 8 Group II lenses whereas there was no opacification in 7 of 8 Group III lenses. Near normal mean levels of reduced glutathione and malondialdehyde, were noted within Group III lenses. Conclusion: The methanolic extract of the O. basilicum leaf appears to prevent selenite-induced cataract formation in-vitro.

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In vitro antioxidant and anticataractogenic potential of silver nanoparticles biosynthesized using an ethanolic extract of Tabernaemontana divaricata leaves

Cited by 28 publications

References 62 publications

Ophthalmic Nanosystems with Antioxidants for the Prevention and Treatment of Eye Diseases

Ophthalmic Nanosystems with Antioxidants for the Prevention and Treatment of Eye Diseases

The Antiviral and Virucidal Activities of Voacangine and Structural Analogs Extracted from Tabernaemontana cymosa Depend on the Dengue Virus Strain

A Methanolic Extract of Ocimum basilicum Exhibits Antioxidant Effects and Prevents Selenite-induced Cataract Formation in Cultured Lenses of Wistar Rats

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