In vitro antioxidant and anticancer activity of crude ethanol extract of Mahkota Dewa (Phaleria macrocarpa) leaves

Christina, Yuyun Ika; Nafisah, Wirdatun; Widodo, Widodo; Rifa’i, Muhaimin; Djati, Muhammad Sasmito

doi:10.1063/5.0052692

Cited by 3 publications

(3 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some studies also mention that this plant has a strong antioxidant effect on leaf and fruit extracts because it contains a lot of flavonoids and phenolics [ 18 ]. Our previous study revealed that the crude extract of P. macrocarpa leaves contains a high phenolic and flavonoid content and antioxidant activity that may be contributed to its cytotoxicity effect on T47D cells [ 12 ]. The seed of this plant effectively induces a cytotoxicity effect on T47D cells, which is higher than mesocarp and pericarp extract [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Study of Antiproliferative Activity in Different Plant Parts of Phaleria macrocarpa and the Underlying Mechanism of Action

Christina

Rifa’i

Widodo

et al. 2022

The Scientific World Journal

Self Cite

View full text Add to dashboard Cite

Phaleria macrocarpa is a medicinal plant widely used in Indonesian folk medicine to treat several diseases, including cancer. However, the comparative evaluation of various plant parts of P. macrocarpa has not been studied for their anticancer properties on breast cancer. The study aimed to assess the antiproliferative activity of the ethanol extract of various parts of Phaleria macrocarpa against T47D human breast cancer cell lines. Several parts of P. macrocarpa, including pericarp, mesocarp, seed, and leaf, were used to determine the most potent plant part to inhibit the growth of T47D cells. The cytotoxic effects of each plant part were evaluated by WST-1 assay. The apoptotic level of T47D cells was determined by annexin V-FITC-PI and DNA fragmentation assay. Propidium iodide staining and the CFSE assay were used to examine the effect of each extract on cell cycle distribution and cell division, respectively. The relative number of caspase-3, Bax, and Bcl-2 was analyzed by flow cytometry technique. WST-1 assay revealed that P. macrocarpa leaves exhibited the most potent antiproliferative activity ( p < 0.05 ) compared to other plant parts with selectivity only to T47D cells. P. macrocarpa leaves extract effectively induced apoptosis, inhibited proliferation, and arrested the cell cycle of T47D cells. The relative number of caspase-3 was significantly ( p < 0.05 ) increased after being treated with P. macrocarpa leaf extract. P. macrocarpa leaf extract also leads to the dose-dependent accumulation in the Bax/Bcl-2 ratio due to upregulation of Bax and downregulation of Bcl-2. The overall results indicated that P. macrocarpa leaves could inhibit the proliferation of T47D cells and trigger apoptosis through caspase-3 activation and Bax/Bcl regulation. Therefore, P. macrocarpa leaves can be used for breast cancer therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The absorbance of the samples was measured on the BioTek ELISA Microplate Reader (BioTek Instruments, Inc., Winooski, USA) at 490 nm wavelength. The following formula determined the percentage of viability cells [ 12 ]:

…”

Section: Methodsmentioning

confidence: 99%

Comparative Study of Antiproliferative Activity in Different Plant Parts of Phaleria macrocarpa and the Underlying Mechanism of Action

Christina

Rifa’i

Widodo

et al. 2022

The Scientific World Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…The ethanol extract of P. macrocarpa leaves showed cytotoxicity toward T47D breast cancer cell lines with an IC 50 value of 97 µg/ml using the WST-1 assay ( Christina et al, 2021b ). MCF7 cell lines are commonly employed as in-vitro tools in breast cancer research; however, this study showed that T47D cell lines could be a suitable experimental model for elucidating the progesterone-specific effects of a luminal A subtype of breast cancer ( Yu et al, 2017 ).…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

Phaleria macrocarpa (Scheff.) Boerl.: An updated review of pharmacological effects, toxicity studies, and separation techniques

Ahmad¹,

Mazlan²,

Aziz³

et al. 2023

Saudi Pharmaceutical Journal

View full text Add to dashboard Cite

Black Rice Extract Induced Apoptosis and Cell Cycle Arrest on Human Cancer Cell Lines

Hartati¹,

Andryanto²

2022

Curr Res Nutr Food Sci

View full text Add to dashboard Cite

The purpose of the research is to evaluate the anti-cancer outcomes of aqueous black rice (BR) extract on human cancer cell lines; HeLa, T47D, and U2OS. To begin the investigation, the total phenol and flavonoid content of the BR extract was determined. Additionally, the antioxidant activity of the extract was determined using the ferric reducing antioxidant power (FRAP), total antioxidant capacity (TAC), and 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) assays. The cell viability, apoptosis and cell cycle on several human cancer cells were determined after BR extract administration in several doses (BR1; 100 mg/ml, BR2; 200 mg/ml, BR3; 300 mg/ml, BR4; 400 mg/ml, and BR5; 500 mg/ml) using flow cytometry analysis. According to the analysis, the extract had total phenol and flavonoid content are 66.42 mg/g and 11.12 mg/g, respectively. Based on the assay of DPPH (53.19 µg/ml), FRAP (49.86 mg/g), and TAC (96.70 mg/g), BR extract showed strong antioxidant activity. This study also revealed that BR extract significantly reduced human cancer cell lines viability and selectively (shown to have no effect on PBMC cells) (p<0.05) especially BR5 group (500 g/mL) . The administration of BR extract was most responsive to induce apoptosis in T47D cells, up to 93.64 % in BR5 close to cisplatin-induced apoptosis effect, compared to HeLa and U2OS cells. The study also found BR extract induced the cell (HeLa, T47D, and U2OS) to arrest or in the phase G0/G1. Hence, BR extract was a food-based cancer treatment with a promising effect to inhibit cancer cell growth and progression.

show abstract

In vitro antioxidant and anticancer activity of crude ethanol extract of Mahkota Dewa (Phaleria macrocarpa) leaves

Cited by 3 publications

References 9 publications

Comparative Study of Antiproliferative Activity in Different Plant Parts of Phaleria macrocarpa and the Underlying Mechanism of Action

Comparative Study of Antiproliferative Activity in Different Plant Parts of Phaleria macrocarpa and the Underlying Mechanism of Action

Phaleria macrocarpa (Scheff.) Boerl.: An updated review of pharmacological effects, toxicity studies, and separation techniques

Black Rice Extract Induced Apoptosis and Cell Cycle Arrest on Human Cancer Cell Lines

Contact Info

Product

Resources

About