In-vitro antioxidant and antibacterial properties of fermentatively and enzymatically prepared chicken liver protein hydrolysates

Abstract

Protein hydrolysates were prepared from chicken liver using fermentation and enzymatic hydrolysis. The lactic acid bacteria Pediococcus acidilactici NCIM5368 was employed in the fermentation process and a commercial protease (Alcalase® 2.5) was used in enzymatic hydrolysis. Chicken liver hydrolysates prepared by fermentation (FCLH) and enzymatic hydrolysis (ECLH) revealed appreciable amounts of protein [55.85 and 61.34 %; on dry weight basis, respectively]. Fermentation and enzymatic hydrolysis resulted in 14.3 and 26.12 % of degree of hydrolysis. Total antioxidant activity, reducing power, scavenging of superoxide, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2-azino-bis-3-ethyl-benzthiazoline-6-sulphonic acid (ABTS) radicals were determined for both FCLH & ECLH. FCLH & ECLH showed total antioxidant activity of 0.99 and 1.13 μg AAE mg −1 proteins, respectively; while, they scavenged 96.14 and

Anti-Hyperglycemic Effects of Green Crab Hydrolysates Derived by Commercially Available Enzymes

“…Proteins and peptides are being widely used as nutraceutical food ingredients in food products and dietary supplements to enhance human health and product value. Many bioactive peptides having antioxidant, anti-hypertensive, and anti-hyperglycemic effects have been derived from enzymatic hydrolysis of protein sources including meat, dairy, fish, and their byproducts [15][16][17][18][19][20][37][38][39]. However, there has been limited investigation into bioactive peptides derived from molluscan and crustacean resources.…”

Effects of brewers’ spent grain protein hydrolysates on gas production, ruminal fermentation characteristics, microbial protein synthesis and microbial community in an artificial rumen fed a high grain diet

“…The DH expresses the number of peptide bonds cleaved as a percentage of total number of peptide bonds in the substrate, with the higher DH expected to have lower molecular weight peptides and vice versa. Enzymatic hydrolysis is gaining importance in producing protein hydrolysates due to its high yield and products with more consistent quality under controllable hydrolysis conditions [17]. Alcalase and avourzyme are commercially available enzymes that have been widely studied for generating bioactive peptides of various protein origin [11,37].…”

“…As a result, alternatives to in-feed antibiotics, using natural sourced materials, have been an area of signi cant research in recent years. Protein hydrolysates of various origins, including animal or agriculture production by-products, have been reported to possess antimicrobial activity [16][17][18], and have the potential to be used as alternatives to antibiotics. Protein hydrolysates of BSG have been reported to possess antibacterial activity towards Escherichia coli O157:H7 [19] and Staphylococcus aureus [20].…”

Development, characterization and determination of biological properties of crocodile hemoglobin (Crocodylus siamensis ) tablets

“…The major protein constituent of crocodile blood is hemoglobin (Hb), which not only plays a crucial role in oxygen transport, but further shown to possess antibacterial and antioxidant properties (Hoffman et al, ; Jandaruang et al, ; Maijaroen, Anwised, Klaynongsruang, Daduang, & Boonmee, ; Pakdeesuwan et al, ; Srihongthong et al, ). Apart from intact Hb, ingested Hb upon digestion in the gastrointestinal tract might improve their biological activities which yielded from alternative resulted peptide fragments (Chakka, Elias, Jini, Sakhare, & Bhaskas, ; Liu, Kong, Jiang, Cui, & Liu, ; Pakdeesuwan et al, ). Using this concept, thus, the feasibility of preparing tableted crocodile Hb as a protein‐based food supplement and the impact of different formulations on biological activity and shelf‐life of the derived tablets were investigated.…”