In-vitro Antioxidant and Antibacterial Activity of Methanolic Extract of Shorea robusta Gaertn. F. Resin

Abstract

The methanolic extract of the resin of Shorea robusta was subjected to investigate its antioxidant and antibacterial properties its utility in free radical mediated diseases including diabetic, cardiovascular, cancer etc. The methanol extract of the resin was tested for antioxidant activity using scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazil) radical method, reducing power by FeCl3 and antibacterial activity against gram positive and gram negative bacteria using disc diffusion method. The phytochemical screening considered the presence of triterpenoids, tannins and flavoniods. Overall, the plant extract is a source of natural antioxidants which might be helpful in preventing the progress of various oxidative stress mediated diseases including aging. The half inhibition concentration (IC50) of resin extract of Shorea robusta and ascorbic acid were 35.60 µg/ml and 31.91 µg/ml respectively. The resin extract exhibit a significant dose dependent inhibition of DPPH activity. Antibacterial activity was observed against gram positive and gram negative bacteria in dose dependent manner.Key Words: Shorea robusta, antioxidant, antibacterial, Disc-diffusion, DPPH.

Phytochemical and Antimicrobial Screening of Bark Extract of Shorea robusta (Sal)

“…Some phytochemicals are supposed to suppress growth and development of microorganism, bacterial cell membrane disarrangement, halting microbial metabolism and modifying microbial genetic expression [31]. Investigators have proved antibacterial activity of S. robusta extracts against bacterial pathogens [32]. In one study, S. robusta extract exhibited antibacterial activity against different clinical pathogens [2].…”

Green Synthesis, Characterization and Application of Natural Product Coated Magnetite Nanoparticles for Wastewater Treatment

“…Free radical scavenging activity was estimated by the DPPH scavenging assay [ 49 , 50 ]. 10.14 µM solution of DPPH in methanol was added to 500 µL MNPs solutions in methanol (total volume was 5 mL) in different concentrations (0.06 mg/mL, 0.25 mg/mL, 0.57 mg/mL, 1.00 mg/mL, 1.57 mg/mL) and the activity was observed at 517 nm after keeping the solutions in dark for about 30 min.…”

Connection Approaches Between Traditional and Modern Pharmacological Profile of Shorea Robusta Gaertn. F.: A Review

“…The resin extract exhibits a significant dose-dependent inhibition of DPPH activity. Investigators have been also proved of S. robusta antibacterial activity which was observed against Gram-positive and Gram-negative bacteria in dose dependent manner [37]. Banerjee et al carried out the study that focuses on phytochemical analysis and antimicrobial effects of lal dhuna, a natural resin secreted from S. robusta (Sal).…”