In vitro antioxidant and anti-inflammatory effects of brewers' spent grain protein rich isolate and its associated hydrolysates

McCarthy, Aoife L.; O’Callaghan, Yvonne C.; Connolly, Alan; Piggott, Charles O.; Fitzgerald, Richard J.; O’Brien, Nora M.

doi:10.1016/j.foodres.2012.10.022

Cited by 69 publications

(52 citation statements)

References 47 publications

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“…Indeed, as mentioned previously, essential amino acids represent~30% of the total protein content, with lysine being abundant (9). McCarthy et al (51) examined the in-vitro antioxidant and anti-inflammatory potential of a BSG protein-rich isolate and associated enzymatically produced hydrolysates. Milk-based products have been the source of the greatest number of bioactive peptides isolated to date; however, increasingly, plant-based materials are being examined as a source of such hydrolysates.…”

Section: Health Benefits Of Bsg and Its Constituentsmentioning

confidence: 99%

Brewers' spent grain: a review with an emphasis on food and health

2016

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Brewers' spent grain (BSG) is the most abundant by‐product generated in the beer‐brewing process. This material consists of the barley grain husks obtained as solid residue after the production of wort. BSG is rich in fibre and protein and, to date, the main use for the elimination of this by‐product has been as an animal feed. However, because of its nutritional content, BSG is of interest for application and fortification of human food products, particularly in view of its low cost and availability in large amounts. In addition, the importance of BSG as an ingredient and potential source of health‐promoting bioactive components is beginning to be recognised. The investigation of alternative uses of BSG is pertinent, not only from the perspective of the brewer who can benefit from valorisation of this by‐product, but also from an environmental perspective as the recycling and re‐use of industrial wastes and by‐products has become increasingly important. This review presents the current knowledge on BSG, covering its production, composition and methods for the release of valuable components, and focuses on the potential health benefits attributed to its constituents and the use of this brewer by‐product in food applications. Copyright © 2016 The Institute of Brewing & Distilling

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Section: Health Benefits Of Bsg and Its Constituentsmentioning

confidence: 99%

Brewers' spent grain: a review with an emphasis on food and health

2016

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“…Ekmekcioglu et al (1999) studied the effect of coffee in Caco-2 cells and no cytotoxicity was observed. Other by-products, like brewers' spent grain, a protein-rich by-product of the brewing industry, have been tested for their cytotoxicity effect (McCarthy et al, 2013). McCarthy et al (2013) studied the cytotoxicity effect of Brewers' spent grain, against U937 and Jurkat T cells and concluded that cytotoxicity is low.…”

Section: 36mentioning

confidence: 99%

“…Other by-products, like brewers' spent grain, a protein-rich by-product of the brewing industry, have been tested for their cytotoxicity effect (McCarthy et al, 2013). McCarthy et al (2013) studied the cytotoxicity effect of Brewers' spent grain, against U937 and Jurkat T cells and concluded that cytotoxicity is low. Over the three peel ethanolic extracts analyzed (Punica granatum (pomegranate), Nephelium lappaceum (rambutan), and Garcinia mangostana (mangosteen), only rambutan may be considered potentially useful as a source of natural antioxidants for food or drug product because of its high antioxidant activity and non-toxic property to normal cells, at concentrations above 100 mg/mL (Okonogi et al, 2007).…”

Section: 36mentioning

confidence: 99%

Coffee silverskin: A possible valuable cosmetic ingredient

Rodrigues

Palmeira‐de‐Oliveira

Neves

et al. 2014

Pharmaceutical Biology

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Context: Currently, there is a great tendency in cosmetic area to use natural extracts. Coffee silverskin (CS) is the most abundant solid by-product generated during roasting of coffee processing. Objectives: To evaluate different CS extracts as promising cosmetic ingredients, regarding antioxidant, antimicrobial, and cytotoxic properties. Materials and methods: Aqueous, hydroalcoholic and ethanolic CS extracts were obtained by an environmentally friendly procedure considering costs and pollution. Extracts were characterized for total phenolic and flavonoid contents (TPC and TFC, respectively), antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), antimicrobial activity expressed as minimal inhibitory concentration (MIC) and cytotoxicity using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) and lactate dehydrogenase (LDH) assays in two skin cell lines (fibroblasts and keratinocytes). Results: The TPC of extracts was 18.33-35.25 mg of gallic acid equivalents per g of material on a dry basis (mg GAE/g db). The TFC of extracts was 1.08-2.47 mg cathechin equivalents per g dry material (mg CE/g db). The antioxidant activity was high, with values ranging between 95.95 and 216.40 mmol Fe 2+ /g for aqueous and alcoholic samples, respectively. Preliminary assays for antimicrobial potential showed that extracts display antibacterial activity. The MIC varied from 31.3 to 250 mg/mL for Gram-positive, and from 31.3 to 1000 mg/mL for Gram-negative. Extracts did not affect in vitro cell viability, with values near 100% in all concentrations tested. Conclusion: Results seem show that CS is a safe source of natural antioxidants with antifungal and antibacterial activity and no cytotoxicity, with potential usefulness for cosmetic applications.

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“…Many phenolic compounds are covalently bound in the malt residues that remain unexploited during brewing process (Connolly, Piggott, & Fitzgerald, ). Other bioactive compounds found in BSG include secondary metabolites such as antibiotics, mycotoxins, alkaloids (Athanasios, Georgios, & Michael, ), food grade pigments, plant growth factors (Mussatto, Dragone, & Roberto, ), flavonoids (Fărcaş et al, ), ferulic and p‐coumaric acid (Mussatto, Dragone, & Roberto, ), compounds known to have antioxidant (Ghafoor, ), antimutagenic (Spinelli, Conte, Lecce, Padalino, Alessandro Del Nobile, ), anti‐allergenic (Gupta, Abu‐Ghannam, & Gallaghar, ), anti‐inflammatory (McCarthy et al, ), and anti‐microbial properties (Meneses, Martins, Teixeira, & Mussatto, ). The several beneficial effects of the enlisted components make BSG a lucrative source of natural additives like antioxidants that could replace the toxic synthetic antioxidants (Meneses et al, ).…”

Section: Introductionmentioning

confidence: 99%

Barley-wheat brewers' spent grain: A potential source of antioxidant rich lipids

Parekh

Khanvilkar

Naik

2017

J Food Process Preserv

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Brewers' spent grain (BSG), a brewery by‐product, seldom finds high value applications. The present work investigated functionality of BSG oil fractions through evaluation of its 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) quenching ability. The scavenging activity of BSG oil was 71.60% and was more than its polar, nonpolar and unsaponified fractions (61.14, 52.42, and 34.47%). The IC50 of the BSG oil (7.39 µg mL−1) was 1000 times lower than common antioxidants—Ascorbic acid (2.86 mg mL−1) and BHT (2.60 mg mL−1), establishing its superior antioxidant activity. The total phenolic content of oil was found to be 1.22 mg GAE per ml of oil while its protein content was found to be between 10.2 and 11.0 mg mL−1. EPR spectroscopy showed 100% quenching of DPPH at 0.5% (w/v) of oil. GCMS revealed the presence of anti‐oxidants. The accelerated oxidation study showed acceptable values for peroxide (till 6 hr) and anisidine (1 hr) tests. Practical applications Brewer' spent grain (BSG) comprises residual constituents of the brewing process that have been utilized for low value applications. The present study revealed a number of bioactive compounds (antioxidants and phenolic compounds) and volatiles in the BSG oil. The antioxidant functionality of this phytochemical rich extract can be utilized for nutraceutical applications to reduce free radical damage in humans. For food preservation, the oil can be employed to reduce browning, rancidity and other deterioration reactions. Thus oil extracted from waste by‐product can be used for high value applications.

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In vitro antioxidant and anti-inflammatory effects of brewers' spent grain protein rich isolate and its associated hydrolysates

Cited by 69 publications

References 47 publications

Brewers' spent grain: a review with an emphasis on food and health

Brewers' spent grain: a review with an emphasis on food and health

Coffee silverskin: A possible valuable cosmetic ingredient

Barley-wheat brewers' spent grain: A potential source of antioxidant rich lipids

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