In vitro antioxidant activity of hydro alcoholic extract from the fruit pulp of Cassia fistula Linn.

Abstract

The present study is aimed to investigate antioxidant activity of the extracts of Cassia fistula Linn. (Leguminosae) fruit pulp. Cassia fistula Linn., a Indian Laburnum, is widely cultivated in various countries and different continents including Asia, Mauritius, South Africa, Mexico, China, West Indies, East Africa and Brazil as an ornamental tree for its beautiful bunches of yellow flowers and also used in traditional medicine for several indications. The primary phytochemical study and in vitro antioxidant study was performed on hydro alcoholic extract of fruit pulp. Phytochemical screening of the plant has shown the presence of phenolic compounds, fatty acids, flavonoids, tannins and glycosides. Phenolic content was measured using Folin-Ciocalteu reagent and was calculated as gallic acid equivalents. Antiradical activity of hydro alcoholic extract was measured by DPPH (2,2-diphenyl-1- picrylhydrazyl) assay and was compared to ascorbic acid. Ferric reducing power of the extract was also evaluated by Oyaizu method. In the present study, three methods were used for evaluation of antioxidant activity. First two methods were for direct measurement of radical scavenging activity and third method to evaluate the reducing power. Results indicate that hydro alcoholic fruit pulp extracts have marked amount of total phenols which could be responsible for the antioxidant activity. These in vitro assays indicate that this plant extract is a significant source of natural antioxidant, Cassia fistula fruit pulp extract shows lower activity in DPPH and total phenol content as compared with standard which might be helpful in preventing the progress of various oxidative stresses.

Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extract

“…Therefore, it can be concluded that this C. fistula flower extract can reduce hyperpigmentation in human skin. Previous studies have shown that some parts of the C. fistula plant exhibited anti-oxidant activity [36–38]. The aqueous and methanolic extracts of the C. fistula bark showed the free radical scavenging effect of DPPH in a dose dependent manner [38].…”

“…The aqueous and methanolic extracts of the C. fistula bark showed the free radical scavenging effect of DPPH in a dose dependent manner [38]. The hydroalcoholic extract of the C. fistula flower and fruit pulp showed antioxidant activity by inhibiting DPPH and hydroxyl radicals [36, 37]. Additionally, our study on the anti-oxidant activity of the butanolic extract of the C. fistula flower similarly displayed the free radical scavenging effect of DPPH and ABTS in a dose dependent manner.…”

A Novel Cassia fistula (L.)-Based Emulsion Elicits Skin Anti-Aging Benefits in Humans

“…Further, as compared to synthetic topical products, natural products are mild, biodegradable and display low toxicity [43].…”

“…fistula L has been reported as a medicinal plant, notably in the treatment of skin and inflammatory diseases [40], particularly due to its relatively rich content of polyphenols and Vitamin C [5], which are known to highly contribute to anti-oxidant activity [43]. Interestingly, considering that the emulsion stability refers to the ability of an emulsion to resist change in its properties over time [58], no significant stability changes were observed during our study period of three months, when ANOVA statistical tests were respectively applied (data not shown).…”

“…Recently, we have shown that C. fistula extracts, which were rich in certain polyphenols (i.e., catechins), exhibited good potential to treat the skin of adult Asian patients with melasma (n = 50) compared to placebo (i.e., without the plant extract), due to its capacity to significantly decrease the tyrosine activity-mediated melanin level [5]. Also, the hydro-alcoholic extract from the fruit pulp of C. fistula displayed an in vitro antioxidant/radical scavenging activity (i.e., 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl aka DPPH activity), certainly due to its relatively rich content of phenolic compounds, fatty acids, flavonoids, tannins and glycosides [42,43].…”