In vitro antioxidant activity of two different extracts of <i>Moringa oleifera</i> leaves from Caribbean St. Lucia Island

Fejér, Jozef; Kron, Ivan; Gruľová, Daniela; Eliašová, Adriana

doi:10.17660/actahortic.2021.1306.17

Cited by 2 publications

(4 citation statements)

References 15 publications

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“…The working FRAP reagents and other reagents were prepared according to the published method [ 25 ]. The only change was in the increase of hydrochloric acid concentration to 50 mmoL.L −1 for dissolving of 10 mmoL.L −1 TPTZ (2,4,6-tripyridyl-s-triazine) [ 31 ]. The activity was expressed in Gallic acid equivalents GAE (FRAP [µmol.L −1 ] in Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

New Mutant Amaranth Varieties as a Potential Source of Biologically Active Substances

Fejér

Kron²,

Eliašová

et al. 2021

Antioxidants

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Amaranth species represent a diverse group of plants. Many of them are a rich source of secondary metabolites with many positive biological effects. Total phenolic, total flavonoid and rutin content, antioxidant activity against superoxide and hydroxyl radicals, FRAP (Ferric-reducing ability of plasma) assay and DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical scavenging assay were determined in ethanol extracts of dried leaves of the new Slovak amaranth varieties ‘Pribina’ and ‘Zobor’. The amount of total phenolic substances (‘Pribina’ GAE 38.3 mg.g−1 DM and ‘Zobor’ GAE 26.1 mg.g−1 DM), content of total flavonoids (‘Pribina’ QE 26.5 mg.g−1 DM and ‘Zobor’ QE 20.3 mg.g−1 DM) and rutin (‘Pribina’ 50.8 mg.g−1 DM and ‘Zobor’ 15.2 mg.g−1 DM) were higher in the variety ‘Pribina’, compared to the variety ‘Zobor’. A statistically higher antioxidant activity against superoxide radical (1.63%·mg−1g−1 DM), hydroxyl radical (3.20%.mg−1g−1 DM), FRAP assay (292.80 µmol.L−1·mg−1.g−1 DM) and DPPH (54.2 ± 1.78 µg.mL−1 DM) were detected in the ‘Pribina’ variety. Antiradical and antioxidant activities of both extracts showed high positive correlations in relation to the content of total phenolic substances, total flavonoids and rutin. Amaranth is an undemanding crop on specific environmental conditions and is resistant to abiotic and biotic stress.

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Section: Methodsmentioning

confidence: 99%

New Mutant Amaranth Varieties as a Potential Source of Biologically Active Substances

Fejér

Kron²,

Eliašová

et al. 2021

Antioxidants

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“…The experiment was prepared based on the published method [73] with slight modifications presented in previous publications [67,74,75]. Phosphate buffer (PB, sodium dihydrogenphosphate dihydrate and disodiumhydrogen phosphate dodecahydrate) in a concentration of 0.05 mol/L and pH 7.4 was used with 0.1 mmol/L Na 2 EDTA.…”

Section: Superoxide Anion Radical Scavenging Activitymentioning

confidence: 99%

“…The experiment for scavenging hydroxyl radicals based on deoxyribose was inspired by the published method [76] with small modifications [67,74,75]. Phosphate buffer (PBS, NaH 2 PO 4 /Na 2 HPO 4 ) 0.05 mol/L pH 7.4 was used with 0.1 mol/L of NaCl and 9 mmol/L 2-deoxyribose.…”

Section: Hydroxyl Radical Scavenging Activitymentioning

confidence: 99%

“…The working FRAP reagents and other reagents were prepared according to the published method [77]. The only change was the increase in hydrochloric acid concentration to 50 mmoL•L −1 for dissolving 10 mmoL•L −1 TPTZ (2,4,6-tripyridyl-s-triazine) [74,75]. Aqueous solutions of iron (II) sulfate heptahydrate in the concentration range of 0-900 mmol L −1 were used for calibration at 600 nm (r = 0.9997).…”

Section: Ferric Reducing Ability Of Plasma (Frap) Assaymentioning

confidence: 99%

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Does the Invasive Heracleum mantegazzianum Influence Other Species by Allelopathy?

Gruľová,

Baranová,

Eliašová

et al. 2024

Plants

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Heracleum mantegazzianum is an invasive species in middle Europe. The mode of action of its invasiveness is still not known. Our study focuses on observation of potential allelopathic influence by the production and release of phytochemicals into its environment. Plant material was collected four times within one season (April, May, June, July 2019) at locality Lekárovce (eastern Slovakia) for comparison of differences in composition and potential allelopathy. Water extracts from collected samples were used for different biological assays. The total phenols and flavonoids were determined spectrophotometrically. The profile and content of phenolic components, including coumarins, were determined by two techniques of liquid chromatography along with in vitro evaluation of the free radical scavenging activity of extracts (DPPH, Hydroxyl, Superoxide, and FRAP). The changes in composition in extracts in different seasonal periods were evident as well as potential phytotoxic activity in some concentrations on specific model plants. The slight antioxidant activity was noted. The invasiveness of the current species could be supported by the excretion of its phytochemicals into its surroundings and by different modes of action influencing living organisms in its environment.

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In vitro antioxidant activity of two different extracts of Moringa oleifera leaves from Caribbean St. Lucia Island

Cited by 2 publications

References 15 publications

New Mutant Amaranth Varieties as a Potential Source of Biologically Active Substances

New Mutant Amaranth Varieties as a Potential Source of Biologically Active Substances

Does the Invasive Heracleum mantegazzianum Influence Other Species by Allelopathy?

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