In Vitro Antioxidant Activity of Areca Nut Polyphenol Extracts on RAW264.7 Cells

Yi, Shuhan; Zou, Luyan; Li, Zongjun; Sakao, Kozue; Wang, Yuanliang; Hou, De‐Xing

doi:10.3390/foods11223607

Cited by 14 publications

(13 citation statements)

References 45 publications

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“…It is mostly used to stimulate cells to produce inflammation. ANP has antioxidant and anti-inflammatory effects; it has been demonstrated in previous studies that ANP attenuates cellular oxidative stress and inflammation induced by LPS through the Nrf2/HO1 and MAPK pathways [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Ameliorative Effect of Areca Nut Polyphenols on Adverse Effects Induced by Lipopolysaccharides in RAW264.7 Cells

Zou,

Yi,

Wang

2024

Molecules

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In Asian regions, areca nuts are tropical fruits that are extensively consumed. The areca nut contains a lot of polyphenols and its safety is unknown. In this research, we investigated the effects of lipopolysaccharides (LPS) and areca nut polyphenols (ANP) on normal RAW264.7 cells. The results showed that LPS stimulated adverse effects in normal cells by affecting cytokine production. The GO analysis results mainly affected DNA repair, cell division, and enzyme activities. In the KEGG analysis results, the NOD-like receptor signaling pathway, which is related to NF-κB, MAPK, and the pro-inflammatory cytokines, is the most significant. In the protein–protein interaction network (PPI) results, significant sub-networks in all three groups were shown to be related to cytokine–cytokine receptor interaction. Collectively, our findings showed a comprehensive understanding of LPS-induced toxicity and the protective effects of ANP by RNA sequencing.

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Section: Discussionmentioning

confidence: 99%

Ameliorative Effect of Areca Nut Polyphenols on Adverse Effects Induced by Lipopolysaccharides in RAW264.7 Cells

Zou,

Yi,

Wang

2024

Molecules

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“…ARE has been also reported to cause the accumulation of reactive oxygen species (ROS) which further cause cholesterol accumulation in the liver, leading to NAFLD [ 37 , 38 ]. Previous study revealed that ANE containing both polyphenols and ARE had a ROS scavenging ability and antioxidant ability [ 39 , 40 ], and ANP containing only polyphenols significantly scavenged LPS-induced ROS [ 41 ]. To provide a reliable understanding of the function and safety of the areca nut, we assessed the effects of whole areca nut extract, polyphenol extract, and ARE on a WD-induced NAFLD mouse model by investigating the changes in metabolic characters and gut microbiota.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Areca Nut Bioactivities in Western Diet-Induced Mice NAFLD Model

Chen

Sakao

et al. 2023

Nutrients

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The areca nut is often consumed as a chewing food in the Asian region. Our previous study revealed that the areca nut is rich in polyphenols with high antioxidant activity. In this study, we further assessed the effects and molecular mechanisms of the areca nut and its major ingredients on a Western diet-induced mice dyslipidemia model. Male C57BL/6N mice were divided into five groups and fed with a normal diet (ND), Western diet (WD), WD with areca nut extracts (ANE), areca nut polyphenols (ANP), and arecoline (ARE) for 12 weeks. The results revealed that ANP significantly reduced WD-induced body weight, liver weight, epididymal fat, and liver total lipid. Serum biomarkers showed that ANP ameliorated WD-enhanced total cholesterol and non-high-density lipoprotein (non-HDL). Moreover, analysis of cellular signaling pathways revealed that sterol regulatory element-binding protein 2 (SREBP2) and enzyme 3-hydroxy-3-methylglutaryld coenzyme A reductase (HMGCR) were significantly downregulated by ANP. The results of gut microbiota analysis revealed that ANP increased the abundance of beneficial bacterium Akkermansias and decreased the abundance of the pathogenic bacterium Ruminococcus while ARE shown the opposite result to ANP. In summary, our data indicated that areca nut polyphenol ameliorated WD-induced dyslipidemia by increasing the abundance of beneficial bacteria in the gut microbiota and reducing the expressions of SREBP2 and HMGCR while areca nut ARE inhibited this improvement potential.

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“…Globally, the areca nut is the fourth most commonly used psychoactive substance after tobacco, alcohol and caffeine [3,4]. Areca nuts can help with resistance to bacteria, removal of parasites and facilitation of digestion [5]. Estimated 600 million people consume areca nuts, predominantly in the southern parts of Asia such as China (Hainan and Taiwan), Vietnam, Indonesia, Myanmar and the Pacific regions [6].…”

Section: Introductionmentioning

confidence: 99%

Edible Quality Analysis of Different Areca Nuts: Compositions, Texture Characteristics and Flavor Release Behaviors

Yang

Chen

et al. 2023

Foods

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The areca nut is one of the most important cash crops in the tropics and has substantial economic value. However, the research information about the edible quality of different areca nuts is still insufficient. This study compared the composition, texture characteristics and flavor release behaviors of four different areca nuts (AN1, AN2, AN3 and AN4) and two commercially dried areca nuts (CAN1 and CAN2). Results showed that AN1 had higher soluble fiber and lower lignin, which was the basis of its lower hardness. Meanwhile, the total soluble solid (TSS) of AN1 was the highest, which indicated that AN1 had a moister and more succulent mouthfeel. After the drying process, the lignification degree of AN1 was the lowest. Through textural analyses, the hardness of AN1 was relatively low compared to the other dried areca nuts. AN1, CAN1 and CAN2 had higher alkaline pectin content and viscosity, and better flavor retention, which indicated better edible quality. The present study revealed the differences of various areca nuts and provided vital information to further advance the study of areca nuts.

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In Vitro Antioxidant Activity of Areca Nut Polyphenol Extracts on RAW264.7 Cells

Cited by 14 publications

References 45 publications

Ameliorative Effect of Areca Nut Polyphenols on Adverse Effects Induced by Lipopolysaccharides in RAW264.7 Cells

Ameliorative Effect of Areca Nut Polyphenols on Adverse Effects Induced by Lipopolysaccharides in RAW264.7 Cells

Assessment of Areca Nut Bioactivities in Western Diet-Induced Mice NAFLD Model

Edible Quality Analysis of Different Areca Nuts: Compositions, Texture Characteristics and Flavor Release Behaviors

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