In vitro antioxidant activity and in vivo antifatigue effect of layered double hydroxide nanoparticles as delivery vehicles for folic acid

Qin, Lili; Wang, Wenrui; You, Shengyong; Dong, Jingmei; Zhou, Yunhe; Wang, Jibing

doi:10.2147/ijn.s74306

Cited by 37 publications

(26 citation statements)

References 44 publications

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“…As it is reported, RSV-SLN can display a sustained release effect, which will help to improve the practical delivery of RSV [16]. RSV molecules are highly stable when they are entrapped in SLN, thereby protecting RSV from degradation in biochemical reactions [32]. Additionally, the potential penetrating ability and cell uptake capacity of RSV-SLN affect the tissue accumulation of RSV that may be responsible for the effects of RSV-SLN in muscle tissue [15].…”

Section: Discussionmentioning

confidence: 89%

Resveratrol-Loaded Solid Lipid Nanoparticle Supplementation Ameliorates Physical Fatigue by Improving Mitochondrial Quality Control

Sun

Zhou

et al. 2019

Crystals

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Resveratrol (RSV) has various pharmacological effects; however, few studies have directly addressed the possible antifatigue effects of long-term endurance exercise. The clinical use of RSV is limited by its poor water solubility and extremely short plasma half-life. Solid lipid nanoparticles (SLNs) are considered as reasonable drug delivery systems to overcome some of these drawbacks and expand its applications. In this study, RSV-SLNs were successfully prepared through emulsification and low-temperature solidification. Results showed that RSV-SLN supplementation effectively enhanced endurance performance. RSV-SLN supplementation might enhance mitochondrial function by ameliorating mitochondrial quality control (QC), which was superior to RSV application. These results revealed an unexpected role of RSV-SLN compared with RSV in terms of linking nutrient deprivation to mitochondrial oxidant production through mitochondrial QC. A mitochondrion-mediated pathway was likely involved in RSV-SLN, thereby improving endurance performance. Overall, this study highlighted new possibilities for anti-physical-fatigue strategies.Crystals 2019, 9, 559 2 of 15 expand the applications of RSV to resolve some of these problems. Solid lipid nanoparticles (SLNs) are considered as an efficient drug delivery system because of their good physicochemical properties [9,10]. SLNs, which can be metabolized by many organisms, can modulate drug release [11][12][13]. SLNs can efficiently protect encapsulated resveratrol drugs in the biological environment and improve their physiochemical properties [14][15][16]. Hence, this study evaluated the effect of RSV-loaded SLN (RSV-SLN) supplementation on exercise performance to explore its possible mechanisms.Maintaining mitochondrial function involves mitochondrial biogenesis, mitophagy, fusion, and fission. The integration of this series of processes reflects mitochondrial quality control (QC). We hypothesized that chronic fatigue states induced by excessive endurance exercise might be linked to injured mitochondrial QC. This study was the first to explore the effects of RSV-loaded SLNs on gene regulation that involves mitochondrial QC following excessive endurance exercise in mice. These data improved the understanding of the role of RSV in a nanometer form as mitochondrial nutrition during successive sessions of prolonged endurance exercise. Materials and Methods Preparation of RSV-SLNsRSV-SLNs were produced by emulsification and low-temperature solidification. In brief, RSV (150 mg) (Aladdin Industrial Corporation, Shanghai, China), lecithin (100 mg), and stearic acid (200 mg) (Shanghai Chemical Reagent Company, Shanghai, China) were dissolved in 10 mL of chloroform in glass bottles as an organic phase through ultrasound. Myrj 52 (Sigma-Aldrich Co., St Louis, MO, USA) was dissolved in 30 mL of distilled water and heated to 75 ± 2 • C in a water bath as an aqueous phase. Under 1000 rpm mechanical stirring, the organic phase was injected into the hot water phase, and the solution was kept at 75 • ...

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Section: Discussionmentioning

confidence: 89%

Resveratrol-Loaded Solid Lipid Nanoparticle Supplementation Ameliorates Physical Fatigue by Improving Mitochondrial Quality Control

Sun

Zhou

et al. 2019

Crystals

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“…Its lactim form has a hydroxyl group on the purine type ring. When folic acid reacts with oxidizing free radicals, this hydroxyl group can play an important role in inhibiting the oxidation effect (Qin et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Tratment With Folic Acid Increases Paraoxonase 1 Activity Thereby Improving Chronic Kidney Disease

Matsumoto¹,

Maes²,

Michelin³

et al. 2020

Preprint

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Introduction: Increased oxidative stress, including elevated homocysteine (Hcy) plasma levels, and lowered levels of antioxidants participate in the pathophysiology and progression of chronic kidney disease (CKD). Paraoxonase (PON)1 activity and folic acid are antioxidants which play a role in Hcy metabolism. However, there are no data whether, in CKD, treatment with folic acid improves glomerular filtration rate (GFR) through effects on PON1 activity and Hcy concentrations. Methods: In the current study, we determined PON1 genotypes and activity, Hcy and estimated GFR (eGFR) both before and after treatment with folic acid (5 mg/d) versus no treatment during three consecutive months in 113 outpatients with CKD classified into stages 4, 3b and 3a. Results: PON1 CMPAase and AREase activities were significantly lower in patients allocated to CKD stage 4 as compared with stages 3b and 3a. Treatment with folic acid significantly improved eGFR and increased levels of CMPAase and AREase in patients allocated to classes 4 and 3b, but not 3a. The improvement of eGFR was associated with increased CMPAase and AREase activities, while the latter were associated with increased levels of folic acid. Treatment with folic acid significantly reduced plasma Hcy levels and the Hcy/PON1 activity ratio. The effects of folic acid increasing PON1 activities were not mediated by changes in Hcy. Discussion: Treatment of CKD patients in early/intermediate stages of CKD patients improves oxidative stress by rebalancing the prooxidant (Hcy) / antioxidant (PON1 activities) ratio. Treatment with folic acid significantly improves eGFR and these effects are mediated via increased PON1 activities. Treatment with folic acid in phase G3b and G4 may reduce renal disease progression by enhancing antioxidant defenses.

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“…However, vitamin B 12 and folic acid have been shown to be antioxidants and thus might positively influence the levels of CPs [26,27]. The allover effects of vitamin supplementation remain unclear.…”

Section: Discussionmentioning

confidence: 99%

Lowered Levels of Carbonyl Proteins after Vitamin B Supplementation in Patients with Mild Cognitive Impairment and Alzheimer's Disease

Fuchs¹,

Leblhuber²,

Schroth³

et al. 2015

Neurodegener Dis

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Background: The critical role of neuro-inflammation and oxidative stress in the pathogenesis of neurodegenerative diseases such as Alzheimer's disease (AD) has become evident. Objective: The aim of this study is to assess the influence of vitamin supplementation on parameters of oxidative stress, inflammation as well as on cognition in patients with AD and mild cognitive impairment. Methods: In our study, patients with cognitive impairment and healthy controls were enrolled. All patients were intended to receive vitamin supplementation (vitamin B₁, B₆, B₁₂ and folic acid) for 3 months. Mini Mental State Examination (MMSE) and laboratory markers [carbonyl proteins (CPs), malondialdehyde, tryptophan (Trp), kynurenine (Kyn), neopterin, folic acid, vitamin B₁₂ level] were assessed for patients and controls at baseline and after 3 months. After half of the patients had been treated for 3 months, analyses were performed resulting in 3 subgroups: healthy controls without supplementation (15 subjects, 11 females), patients with vitamin supplementation (17 subjects, 10 females) and patients without vitamin supplementation (16 subjects, 9 females; baseline values prior to supplementation). Results: Age was significantly higher for the supplemented group (76.4 ± 6.7 years) compared to vitamin-naïve patients (63.3 ± 13.7 years; p < 0.01). The MMSE score was higher in the supplemented group (23.1 ± 4.8 vs. 20.3 ± 9.5) but did not reach significance. Levels of CPs were significantly higher in the vitamin-naïve patients (p < 0.05). Levels of Kyn and the Kyn/Trp ratio were significantly lower in vitamin-naïve patients compared to the supplemented group (p < 0.05). No significant difference was seen for the other markers. Conclusion: Vitamin supplementation leads to reduced levels of CPs in patients. Pearson's correlation coefficient shows a negative relation (r = -0.69) between CPs and MMSE. Future trials should assess whether CPs might be suitable markers for monitoring of demented patients.

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In vitro antioxidant activity and in vivo antifatigue effect of layered double hydroxide nanoparticles as delivery vehicles for folic acid

Cited by 37 publications

References 44 publications

Resveratrol-Loaded Solid Lipid Nanoparticle Supplementation Ameliorates Physical Fatigue by Improving Mitochondrial Quality Control

Resveratrol-Loaded Solid Lipid Nanoparticle Supplementation Ameliorates Physical Fatigue by Improving Mitochondrial Quality Control

Tratment With Folic Acid Increases Paraoxonase 1 Activity Thereby Improving Chronic Kidney Disease

Lowered Levels of Carbonyl Proteins after Vitamin B Supplementation in Patients with Mild Cognitive Impairment and Alzheimer's Disease

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