In Vitro Antioxidant Activity and Gc-MS Analysis of the Ethanolic Extracts of Terminalia Bellerica Roxb (Baheda)

Abstract

Objective: To investigate the antioxidant activity of ethanolic extract of Terminalia bellerica fruit pulp (TBFP), seed (TBS) and bark (TBB), and identification of phytochemical constituents of said extracts. Methods:The antioxidant potential of T. bellerica (TB) parts was evaluated by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), hydroxyl radical scavenging activity (HRSA) and compared with reference standard quercetin. Lipid peroxidation (LPO), ferric thiocyanate activity (FTC) and reducing power (RP) of the plant extracts were also examined. The phytochemical constituents of said extracts have been quantified by gas chromatography-mass spectrometer (GC-MS) method.Results: Ethanolic extract of TBFP exhibited high phenolic content (254.33) followed by TBS (227.33) and TBB (185) mg/g of GAE. TBFP showed lowest IC 50 for FRSA (62 µg/ml), SARSA (39 µg/ml) and HRSA (27 µg/ml) and highest RP (3.39 ascorbic acid equivalents/ml). TBB showed lowest IC 50 for LPO (48 µg/ml) whereas TBS showed lowest IC50 Conclusion: On the basis of correlation of antioxidant studies with total phenolic content (TPC) and GC-MS analysis of different part of TB, it may be concluded that high concentration of total phenolics and other bioactive phytochemicals of TB extracts make it a potential source of nutraceutical antioxidants.for FTC (109 µg/ml). Predominant phyto-compounds present in TB extracts were quinic acid, gallic acid, ethyl galate, 9, 12 octadecadienoic acid and glucopyranose in varying concentrations as analyzed by GC-MS.

Oxidative Dna Damage Protective and Hepatoprotective Activity of Termi-Nalia Bellerica (Baheda)

“…In fact, at high dose, they were attributed to direct toxic effect and induced cells to death. It is evident from the extent of activity that T. bellerica contains hepatoprotective principles not only in fruits as reported earlier [18,19], but the leaves and bark also contain various bioactive principles which may responsible for the hepatoprotective potential of T. bellerica [7]. Thus, determination of percentage cell viability of plant extracts is a biochemical parameter which indicates towards the potential of medicinal plants for their antioxidant, anti-inflammatory, anticancer and hepatoprotective activities.…”

“…bellerica contains a diverse set of vital phytoconstituents like gallic acid, ethyl galate, quinic acid, octadecanoic acid, glucopyranose, sitosterols etc [7]. Polyphenolic compounds in T. bellerica had dual effects on cells and modulated cell proliferation in a dosedependent manner.…”

“…It is commonly known as Baheda, found in deciduous forests throughout the greater part of India except the dry region of Western India. Gupta et al reported that T. bellerica is the richest source of various bioactive phytochemicals especially polyphenolic compounds [7]. The same group demonstrated that the extracts of T. bellerica exhibited strong and effective in vitro antioxidant activity by chelating metal ions as well as scavenging free radicals.…”

Comparative Study on the Antioxidant Activities of Methanolic and Aqueous Extracts of Terminalia Bellerica

“…It is an integral part of ancient formulation, Triphala which is used for a variety of ailments in Ayurveda. GC-MS analysis revealed that TB contains various polyphenolic and other bioactive compounds such as gallic acid, quinic acid (chlorogenic acid), ethyl galate, 9,12 octadecadienoic acid, glucopyranose, sitosterol, protein, tannins, galactose, glucose, mannitol, fructose, ramnose etc [10]. Antioxidant activities increase proportionally with the polyphenol content, primarily because of their redox properties.…”

Antioxidant, DNA protective and antibacterial activities of Terminalia bellerica extracts

“…Gas chromatography mass spectrometry (GC-MS) analysis revealed that T. bellerica contains various polyphenolic and other bioactive compounds such as gallic acid, quinic acid (chlorogenic acid), ethyl galate, 9,12 octadecadienoic acid, glucopyranose, sitosterol, protein, tannins, galactose, glucose, mannitol, fructose, ramnose etc. (Gupta et al, 2016). Antioxidant activities increase proportionally with the phenolic content, primarily because of their redox properties (Gupta et al, 2015(Gupta et al, , 2016.…”