In vitro antioxidant activity and phenolic contents in methanol extracts from medicinal plants

Guleria, Sanjay; Tiku, A. K.; Singh, Gurjinder; Koul, A. K.; Gupta, Sahil; Rana, Subhash

doi:10.1007/s13562-012-0105-6

Cited by 61 publications

(39 citation statements)

References 24 publications

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“…Himalayas was found quite low and exhibited poor antiradical efficiency. 39 Stem extract of Berberis lycium Royle showed medium radical scavenging activity and contained moderate amount of total phenols and flavonoids. 40 Other species of Berberis also possess good amount of phenolics.…”

Section: S169mentioning

confidence: 97%

Antioxidant Activity, Total Phenolic and Flavonoid Content of Berberis aristata DC. and Berberis thomsoniana C.K. Schneid. from Sagarmatha National Park, Nepal

Bhatt¹,

Wagle²,

Adhikari³

et al. 2018

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Background: Medicinal plants are the major sources of the crude drugs and natural antioxidants. Berberis species have been used in the treatment of various ailments around the world including Nepal. However, systematic study on phytochemistry and pharmacology of Berberis species from Nepal is lacking. Objectives: To evaluate the antioxidant activity, total phenolic and flavonoid contents of Berberis aristata and Berberis thomsoniana from Sagarmatha National Park. Materials and Methods: Antioxidant activity was measured through 2, 2-Diphenyl-1-Picrylhydrazyl assay. Total phenolic and flavonoid content were estimated using Folin-Ciocalteau and aluminum chloride method respectively. Results: Samples showed dose-dependent radical scavenging activity. Radical scavenging activity of the methanolic extracts of different parts of B. aristata and B. thomsoniana ranged from 19.38 to 98.47%, with leaf extracts of B. thomsoniana showing the strongest activity. The total phenolic content of the samples varied from 11.04 to 65.30 mg GAE g -1 dry weight whereas total flavonoid content was in between 2.4 to 16.46 mg quercetin/g dry weight. Conclusion: Among the tested samples, leaf extracts of B. thomsoniana showed the strongest antioxidant activity and contained the highest amount of total phenolic and flavonoid content. ABSTRACT SUMMARY• Present study showed that leaf extract of B. thomsoniana showed promising antioxidant ability and contained higher amount of phenolics and flavonoids.Dr. Lok Ranjan Bhatt is a Senior Scientific Officer at Nepal Academy of Science and Technology (NAST), Khumaltar, Lalitpur, Nepal. He has more than twenty years of experience in the areas of medicinal and aromatic plants, natural products and biomaterials. Currently, he has been working on nutritional composition of wild edible fruits and synthesis and characterization of herbal based polymer micro/nanospheres.

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Section: S169mentioning

confidence: 97%

Antioxidant Activity, Total Phenolic and Flavonoid Content of Berberis aristata DC. and Berberis thomsoniana C.K. Schneid. from Sagarmatha National Park, Nepal

Bhatt¹,

Wagle²,

Adhikari³

et al. 2018

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“…In another study, (Jain et al ., ) reported a FRAP of 80 m m Fe 2+ per g from A. racemous roots extract with 60% methanol in the traditional extraction method, while Guleria et al . () reported a FRAP value of 8.7 μmol Fe (II) per g) with 80% methanol in A. racemosus roots and a value of 9.4 μmol Fe (II) per g of A. adscendens Roxb roots with 80% methanol at 45 °C for 2 h extraction in a conventional extraction method, which further confirmed the values of FRAP are affected by the type of materials. Comprehensive fractionation of Thai A. racemosus was carried out by Kongkaneramit et al .…”

Section: Phytochemistry Of Armentioning

confidence: 59%

“…It is suggested that the different values of IC 50 are contributed by the composition of flavonoids in AR from the different regions. While an EC 50 of DPPH radical scavenging activity of 0.209 mg mL −1 was shown in dry weight of A. racemosus roots and 0.202 mg mL −1 in A. adscendens Roxb roots with 80% methanol extraction at 45 °C for 2 h by shaking extraction methods (Guleria et al ., ). Their results suggest that the extraction parameters and various species of AR used are more important in extracting antioxidant compounds from AR, which should be taken into consideration in further studies.…”

Section: Phytochemistry Of Armentioning

confidence: 97%

Phytochemical compounds and biological activity in Asparagus roots: a review

Zhang

Birch

Pei

et al. 2018

Int J of Food Sci Tech

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Asparagus roots (AR) contain many bioactive compounds and their use in Chinese and Indian traditional medicines is well documented. Both in vitro and in vivo studies have indicated AR extracts act as antidiabetic, antioxidant and hypolipidaemic and depict anti-inflammatory, antibacterial, antiviral, anticarcinogenic activities and also improve cardiovascular and oral health. The physiological effects against wide range of pathologies are due to the wide range of phytochemicals found in AR including polyphenols, saponins and polysaccharides. The aim of this review is to present an overview of the functional, medical and physiological properties of AR bioactives. This review considers the roots of several asparagus species and provides up-to-date information on Asparagus officinalis that has not been extensively reviewed before.

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“…() reported a TPC of 10.0, 25.1 and 4.8 mg GAE per g extract in A. racemosus root extracted at room temperature for 24 h with methanol, n‐butanol and water, respectively. Increasing the concentration of methanol in aqueous alcoholic solutions and extraction time can improve the yield of TPC (Ghimire et al ., ; Jain et al ., ; Guleria et al ., ; Shah et al ., ). Using A. racemosus root, Jain et al .…”

Section: Resultsmentioning

confidence: 99%

“…reported a TPC of 10.0, 25.1 and 4.8 mg GAE per g extract in A. racemosus root extracted at room temperature for 24 h with methanol, n-butanol and water, respectively. Increasing the concentration of methanol in aqueous alcoholic solutions and extraction time can improve the yield of TPC(Ghimire et al, 2011;Jain et al, 2011;Guleria et al, 2013;Shah et al, 2013). Using A. racemosus root,Jain et al (2011) reported a TPC of 4.13 mg GAE / g extract using 60% methanol at room temperature for 4 h whereasGhimire et al (2011) reported a TPC of 45.37 mg GAE/g extract using 80% methanol for 24 h at room temperature.…”

mentioning

confidence: 99%

Effect of solvents on polyphenol recovery and antioxidant activity of isolates of Asparagus Officinalis roots from Chinese and New Zealand cultivars

Zhang

Birch

Hong-yuan

et al. 2018

Int J of Food Sci Tech

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Summary Nine solvents of varying polarity (water, methanol, ethanol, isopropyl alcohol, acetone, n‐butyl alcohol, ethyl acetate, chloroform and petroleum ether) were used to determine the optimal extraction solvent that gives the highest yield of bioactive compounds from six Asparagus Officinalis L root (AR) cultivars from China and New Zealand. The contents of total phenolics (TPC), total flavonoids (TFC), total saponins (TSC) and caffeic acid (CA) were determined. Antioxidant assessments included ferric reducing/antioxidant power (FRAP), 2, 2′‐azinobis‐(3‐ diphenyl‐ethylbenzothiazoline‐6‐sulphonic acid) radical cation (ABTS) and β‐carotene bleaching activity assays. The results indicated that yellow AR from China had the highest content of TPC, TFC, TSC and CA compared to other AR cultivars. The most effective solvents for bioactive compound recoveries and antioxidant activities from A. officinalis L root extraction were ethanol and methanol. The content of CA in ethanol extracts of yellow AR achieved 92.0 mg g−1 DE.

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In vitro antioxidant activity and phenolic contents in methanol extracts from medicinal plants

Cited by 61 publications

References 24 publications

Antioxidant Activity, Total Phenolic and Flavonoid Content of Berberis aristata DC. and Berberis thomsoniana C.K. Schneid. from Sagarmatha National Park, Nepal

Antioxidant Activity, Total Phenolic and Flavonoid Content of Berberis aristata DC. and Berberis thomsoniana C.K. Schneid. from Sagarmatha National Park, Nepal

Phytochemical compounds and biological activity in Asparagus roots: a review

Effect of solvents on polyphenol recovery and antioxidant activity of isolates of Asparagus Officinalis roots from Chinese and New Zealand cultivars

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