In vitro antioxidant activities of Rhodobacter sphaeroides and protective effect on Caco-2 cell line model

An, Jun; Yang, Cui; Li, Zuming; Perkins, David L.; Sun, Jun; Bai, Zhongke; Gao, Liping; Zhang, Michael; Ren, Difeng

doi:10.1007/s00253-018-9497-0

Cited by 17 publications

(6 citation statements)

References 45 publications

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“…Antioxidant potential and protective role of Weissella EPS have also been reported from a variety of EPS isolated from Weissella many strains like Weissella confusa EPSWWC, W. confusa OF126, W. cibaria GA44, W. cibaria YB-1, W. confusa W4 and W. cibaria SJ14 24 – 29 . In vivo antioxidant properties can be studied using various model systems such as cell lines 30 , C. elegans 31 , yeast 32 and mice 33 . Previously, various compounds have been screened for their potential antioxidant properties using Saccharomyces cerevisiae yeast gene deletion mutant model system 32 , 34 – 37 .…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress alleviating potential of galactan exopolysaccharide from Weissella confusa KR780676 in yeast model system

Kavitake

Veerabhadrappa

Sudharshan

et al. 2022

Sci Rep

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In the present study, galactan exopolysaccharide (EPS) from Weissella confusa KR780676 was evaluated for its potential to alleviate oxidative stress using in vitro assays and in vivo studies in Saccharomyces cerevisiae (wild type) and its antioxidant (sod1∆, sod2∆, tsa1∆, cta2∆ and ctt1∆), anti-apoptotic (pep4∆ and fis1∆) and anti-aging (sod2∆, tsa1∆ and ctt1∆)) isogenic gene deletion mutants. Galactan exhibited strong DPPH and nitric oxide scavenging activity with an IC50 value of 450 and 138 µg/mL respectively. In the yeast mutant model, oxidative stress generated by H2O2 was extensively scavenged by galactan in the medium as confirmed using spot assays followed by fluorescencent DCF-DA staining and microscopic studies. Galactan treatment resulted in reduction in the ROS generated in the yeast mutant cells as demonstrated by decreased fluorescence intensity. Furthermore, galactan exhibited protection against oxidative damage through H2O2 -induced apoptosis inhibition in the yeast mutant strains (pep4∆ and fis1∆) leading to increased survival rate by neutralizing the oxidative stress. In the chronological life span assay, WT cells treated with galactan EPS showed 8% increase in viability whereas sod2∆ mutant showed 10–15% increase indicating pronounced anti-aging effects. Galactan from W. confusa KR780676 has immense potential to be used as a natural antioxidant for nutraceutical, pharmaceutical and food technological applications. As per our knowledge, this is the first report on in-depth assessment of in vivo antioxidant properties of a bacterial EPS in a yeast deletion model system.

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Section: Introductionmentioning

confidence: 99%

Oxidative stress alleviating potential of galactan exopolysaccharide from Weissella confusa KR780676 in yeast model system

Kavitake

Veerabhadrappa

Sudharshan

et al. 2022

Sci Rep

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“…Our studies indicated that R. sphaeroides superfine powder has immunomodulatory activity and can enhance the immune function of mice. Besides, In vitro cell experiments were conducted to study the antioxidant activity of R. sphaeroides, and the results showed that R. sphaeroides had antioxidant activity, which could coexist with Caco-2 cells and improve the antioxidant activity of Caco-2 cells under oxidative stress [34]. We hypothesize that R. sphaeroides may exert an antioxidant activity in the gut and increase the anaerobic bacteria Mycoplasma and Anaeroplasma abundance.…”

Section: Discussionmentioning

confidence: 99%

“…R. sphaeroides (CGMCC No. 8513) exhibited significant protective activity against H 2 O 2 -induced oxidative stress in Caco-2 cells [34]. Besides, superfine powder of R. sphaeroides was proved to enhance the immunological function of mice and to improve the antioxidant effect of intestinal epithelial cells.…”

Section: Introductionmentioning

confidence: 96%

The Effects of <i>Rhodobacter sphaeroides</i> on the Composition of Gut Microbiota<i> </i>and Short-chain Fatty Acids in Mice

Yang¹,

Luan²,

An³

et al. 2020

JFNR

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This study aims to determine the effects of Rhodobacter sphaeroides 8513 on the physiological and biochemical indicators, short-chain fatty acids, and gut microbiota of healthy BALB/c mice. Mice were divided into the control group and R. sphaeroides group (5×10 8 CFU/mL). After 28 days of continuous gavage, fecal samples were collected for high-throughput sequencing, then mice were sacrificed and the serum was taken for biochemical analysis. Liver, kidney, and spleen were obtained for organ coefficient calculation, and cecum contents were collected for short-chain fatty acid analysis by gas chromatography. The results showed that there was no significant difference in body weight gain, alanine aminotransferase (ALT) and urea nitrogen (BUN) levels between the control group and R. sphaeroides group, while R. sphaeroides significantly decreased the aspartate aminotransferase (AST) and creatinine (CRE) level. No side effect of R. sphaeroides treatment on the basic physiological health of mice was observed. R. sphaeroides significantly increased the content of acetic acids in the gut of mice, while there were no significant changes in the concentration of butyric and propionic acid. High-throughput sequencing analysis indicated that there was a significant difference in α-diversity between these 2 groups, while there was no significant difference in β-diversity between them. R. sphaeroides not only increased the abundance of anaerobic bacteria of Rhodospirillaceae, Desulfovibrionaceae, and Helicobacter in the gut of mice, but also increased the abundance of the predominant microflora in the gut such as Bacteroidaceae (Bacteroides), Prevotellaceae, Clostridiales_vadinBB60_group (Closporaceae). These results suggested that R. sphaeroides 8513 could modulate the microbial ecology in the gut of mice.

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“…This is not the case, however, for sodium benzoate and sodium tartrate ( Table S1 ) [ 11 ]. Previous studies have revealed the uniquity of “ Rhodobacter sphaeroides ” strain EBL0706 for its outstanding antioxidant capacity [ 12 ] and the ability to synthesize a variety of bioactive substances, such as carotenoids [ 13 , 14 ], chlorophylls [ 15 ], superoxide dismutase [ 16 , 17 ], and vitamin B12 [ 18 ]. In addition, studies have demonstrated the potential of this strain for environmental remediation on pollutants, such as dichlorvos [ 19 ] and oil [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Genome Analysis Provides Molecular Evidence for Reclassification of the Photosynthetic Bacterium Rhodobacter sphaeroides EBL0706 as a Strain of Luteovulum azotoformans

Wang

Sha

et al. 2021

Microorganisms

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In this study, we conducted a genome-wide comparative analysis of a former Rhodobacter sphaeroides strain EBL0706, which is now recorded as Luteovulum sphaeroides EBL0706. The genome of EBL0706 was compared with that of Luteovulum azotoformans ATCC 17025, Luteovulum azotoformans KA25, and Luteovulum sphaeroides 2.4.1. The average nucleotide identity (ANI), tetra nucleotide signatures (Tetra), digital DNA–DNA hybridization (dDDH) values, comparative genome, and phylogenetic analysis proposed that EBL0706 is a strain of Luteovulum azotoformans. Functional annotations identified a total of 4034 protein-coding genes in the genome of EBL0706, including a complete photosynthetic gene cluster. This study provides genomic molecular verification for the strain EBL0706 to be reclassified to Luteovulum azotoformans.

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In vitro antioxidant activities of Rhodobacter sphaeroides and protective effect on Caco-2 cell line model

Cited by 17 publications

References 45 publications

Oxidative stress alleviating potential of galactan exopolysaccharide from Weissella confusa KR780676 in yeast model system

Oxidative stress alleviating potential of galactan exopolysaccharide from Weissella confusa KR780676 in yeast model system

The Effects of <i>Rhodobacter sphaeroides</i> on the Composition of Gut Microbiota<i> </i>and Short-chain Fatty Acids in Mice

Comparative Genome Analysis Provides Molecular Evidence for Reclassification of the Photosynthetic Bacterium Rhodobacter sphaeroides EBL0706 as a Strain of Luteovulum azotoformans

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