In vitro Antioxidant Activities of Various Extracts from Flowers-Leaves Mixture of Algerian Cytisus triflorus

Madoui, Soraya; Charef, Noureddine; Arrar, Lekhmici; Baghianni, Abderrahmane; Khennouf, Seddik

doi:10.9734/arrb/2018/41297

Cited by 8 publications

(6 citation statements)

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“…Furthermore, hydroxyl groups and conjugated structures, which scavenge free radicals through hydrogenation by donating hydrogen atoms, make polyphenols excellent antioxidants. 48,49 Therefore, the fact that the aqueous root extract of L. cornuta possesses antioxidant efficacy may explain its anti-inflammatory effects in managing various inflammatory conditions in traditional medicine.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant, Anti-Inflammatory, Acute Oral Toxicity, and Qualitative Phytochemistry of The Aqueous Root Extract of Launaea cornuta (Hochst. Ex Oliv. & Hiern.)

Akimat

Omwenga

Moriasi

et al. 2021

J Evid Based Complementary Altern Med

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The root and leaf extracts of Launaea cornuta have been locally used in traditional medicine for decades to manage inflammatory conditions and other oxidative-stress-related syndromes; however, their pharmacologic efficacy has not been scientifically investigated and validated. Accordingly, we investigated the in vitro antioxidant activity, anti-inflammatory ( in vitro, ex vivo, and in vivo) efficacy, acute oral toxicity, and qualitative phytochemical composition of the aqueous root extract of L. cornuta. The ferric-reducing antioxidant power (FRAP) and the 2,2-diphenyl-2-pycrylhydrazyl (DPPH) radical scavenging test methods were used to determine the studied plant extract’s antioxidant activity. Besides, the anti-inflammatory efficacy of the studied plant extract was investigated using in vitro (anti-proteinase and protein denaturation), ex vivo (membrane stabilization), and in vivo (carrageenan-induced paw oedema in Swiss albino mice) methods. The studied plant extract demonstrated significant in vitro antioxidant effects, which were evidenced by higher DPPH radical scavenging and FRAP activities, in a concentration-dependent manner ( p < 0.05). Generally, the studied plant extract exhibited significant in vitro, ex vivo, and in vivo anti-inflammatory efficacy, respectively, and in a concentration/dose-dependent manner compared with respective controls ( p < 0.05). Moreover, the studied plant extract did not cause any observable signs of acute oral toxicity, even at the cut-off dose of 2000 mg/Kg BW (LD50 > 2000 mg/Kg BW), and was thus considered safe. Additionally, qualitative phytochemistry revealed the presence of various antioxidant- and anti-inflammatory-associated phytochemicals, which were deemed responsible for the reported pharmacologic efficacy. Further studies to characterise bioactive molecules and their mode(s) of pharmacologic efficacy are encouraged.

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Section: Discussionmentioning

confidence: 99%

Antioxidant, Anti-Inflammatory, Acute Oral Toxicity, and Qualitative Phytochemistry of The Aqueous Root Extract of Launaea cornuta (Hochst. Ex Oliv. & Hiern.)

Akimat

Omwenga

Moriasi

et al. 2021

J Evid Based Complementary Altern Med

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“…The presence of phenolic compounds in these fractions may be explained there reducing power. Relations between Fe 3+ reducing activity and total phenol content have been reported in the literature 10 .…”

Section: Discussionmentioning

confidence: 92%

“…The phenolic compounds extraction was conducted as reported by Madoui et al 10 Aerial parts of A. scoparium (100 gm) were extracted with one liter of methanolwater (85:15 v/v) and kept under agitation overnight at 4 °C the resulting solution was filtered to obtain the first filtrate. This procedure was repeated on the residue using water-methanol (50:50 v/v) under agitation for 4 h to obtain the last filtrate.…”

Section: Preparation Of Plant Extractsmentioning

confidence: 99%

Untitled

2019

IJPSR

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This study aims to evaluate the in-vitro and in-vivo antioxidant activity of different extracts prepared from the aerial parts of Arthrophytum scoparium. Polyphenols flavonoids and tannins contents were determined. The acute toxicity of ASE was carried out based on OECD during two weeks to ascertain the LD 50 . In sub-acute toxicity, a 100, 200 and 400 mg/kg/day extract was orally administrated for 21 days consecutively. The results showed that decoction extract (DEC) was the richest fractions in Polyphenols, flavonoids and tannins content (23.29 mg/GAE, 99.77 mg/QE and 113.33 mg/TAE), respectively. Arthrophytum scoparium hydromethanolic extract (ASE) had the strongest antioxidant activity using DPPH and ABTS scavenging assays with an IC 50 of 0.045 and 0.0017 mg/ml, respectively. Moreover, the β-carotene/linoleic acid bleaching assay showed that ethyl acetate extract (EAE) had the highest inhibiting activity (83.29%). Furthermore, all A. scoparium fractions exhibited a good ferric reducing power. The administrated doses did not undergo changes in general behavior, toxicity or mortality of the tested mice. The LD 50 was found to be superior to 5 g/kg. Daily administration of ASE leads to an increase in GSH level and decreasing the lipid peroxidation in the liver. In-vivo, ASE showed a strong scavenging effect on DPPH and reducing power. In conclusion, A. scoparium extracts exhibited considerable antioxidant effects in-vitro and in-vivo. These findings support the traditional use of this plant as an anti-inflammatory in therapeutics.

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“…The extraction was performed according to Madoui et al (2018) 9 . About 100 g of powdered plant materials was taken in amber colored extraction bottles and soaked with 1 L of methanol-water (85:15 v/v) and kept under agitation overnight at 4°C the resulting solution was filtered to obtain the first filtrate.…”

Section: Preparation Of the Extractsmentioning

confidence: 99%

Anti-Inflammatory Potential Evaluation (In-Vitro and In-Vivo) of Arthrophytum scoparium Aerial Part

Kaddour¹,

Arrar²,

Baghiani³

2020

J. Drug Delivery Ther.

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Arthrophytum scoparium is plant commonly used in folk medicine in Algeria to treat numerous human diseases especially infectious, Rheumatism, diabetes and cancer. However, its anti-inflammatory activity and the underlying mechanisms have not been studied systematically. The present study aims to assess both the in vitro and in vivo anti-inflammatory potential and antioxidant activity of the plant. Results showed that A. scoparium chloroform extract (ChE) contains the largest amount of phenolic and flavonoid compounds. In-vitro bioassay consisted of investigating the effect of the plant extracts against protein denaturation (BSA and egg albumin). In-vivo anti-inflammatory activity was evaluated by measuring the percentage inhibition of carrageenan-induced rat paw edema after oral administration of the extracts. Results showed that inhibition of protein denaturation was maximum in case of A. scoparium crude extract (CrE) with dose dependant manner. Similarly in the in-vivo study, carrageenan induced inflammation was significantly antagonized by CrE with inhibition of 87.02% at 400 mg/kg. CrE extracts showed the highest scavenger effect against hydroxyl and hydrogen peroxide. The CrE showed potent anti-inflammatory activity in both the conditions in vivo and in vitro. In conclusion different A. scoparium extracts scavenged reactive oxygen species efficiently; it may be due to the presence of its flavonoids and polyphenols. These results support the traditional use of this plant. Keywords: Arthrophytum scoparium, polyphenols, anti-inflammatory activity, radical scavenging activity.

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In vitro Antioxidant Activities of Various Extracts from Flowers-Leaves Mixture of Algerian Cytisus triflorus

Cited by 8 publications

References 0 publications

Antioxidant, Anti-Inflammatory, Acute Oral Toxicity, and Qualitative Phytochemistry of The Aqueous Root Extract of Launaea cornuta (Hochst. Ex Oliv. & Hiern.)

Antioxidant, Anti-Inflammatory, Acute Oral Toxicity, and Qualitative Phytochemistry of The Aqueous Root Extract of Launaea cornuta (Hochst. Ex Oliv. & Hiern.)

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Anti-Inflammatory Potential Evaluation (In-Vitro and In-Vivo) of Arthrophytum scoparium Aerial Part

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