In vitro antimicrobial efficiency of a mouthwash containing triclosan/gantrez and sodium bicarbonate

Abstract

Several antiseptic substances have been used as adjuncts to routine mechanical procedures of oral hygiene, based on their antimicrobial effects. The objective of this study was to assess in vitro the antimicrobial efficiency of a mouthwash containing Triclosan/Gantrez and sodium bicarbonate in comparison to both positive and negative controls. Standard strain samples of Escherichia coli, Pseudomonas aeruginosa, Actinomyces viscosus and Bacillus subtilis were used. Samples of Streptococcus mutans and Gram-negative bacilli were collected from 20 volunteers (10 with a clinically healthy periodontium and 10 presenting biofilm-associated gingivitis). Evaluation of the antimicrobial activity was performed by determining the minimal inhibitory concentration (MIC). The results indicated that the test solution inhibited the growth of both Gram-negative and Gram-positive microorganisms from the volunteers’ saliva as well as that of the standard strains at the MIC dilution of 1:20, whereas the MIC dilution of 0.12% chlorhexidine against the same bacteria was 1:80. Thus, even though the tested mouthrinse solution presented an in-vitro antimicrobial activity superior to that of a placebo, it was inferior to that of chlorhexidine.

Present and Future Non-Surgical Therapeutic Strategies for the Management of Periodontal Diseases

“…Pires et al (Pires, et al, 2007) have concluded that a mouthwash containing a combination of Triclosan/Gatrez and sodium bicarbonate has an in-vitro antimicrobial activity superior to that of a placebo, but still inferior to that of chlorhexidine.…”

“…In vitro studies show antimicrobial activity superior to that of a placebo, but inferior to that of chlorhexidine (Pires, et al, 2007) Siegrist et al (Siegrist, et al, 1986) Listerine Peridex Placebo Double blind, controlled clinical trial. After a baseline complete dental prophylaxis,124 healthy adults used the mouthrinse as a supplement to regular oral hygiene for 6 months.…”

Gingivitis Control

“…It is usually combined with either zinc citrate or a copolymer of methoxyethylene and maleic acid, in which form it is effective in reducing plaque and gingivitis (Ciancio, 1987). A non-ionic phenol derivative, Triclosan has a broad spectrum antimicrobial activity against gram positive and gram negative bacteria and has been shown to be beneficial in toothpaste (Pires et al, 2007). Triclosan acts on the microbial cytoplasmic membrane to induce leakage of cellular constituents and bacteriolysis (Rolla et al, 1996).…”

Effect of Citrus aurantifolia swingle essential oils on methyl mercaptan production of Porphyromonas gingivalis

“…23 However, besides being antibacterial in nature, chlorhexidine also has local side effects when used as a mouthwash that is it can cause extrinsic staining on the teeth so that the teeth become dark yellow brown. 21 Based on these research, it can be concluded that the essential oil of lemon peel (Citrus aurantifolia swingle) of concentration 1%, 2%, 3% and 4% can reduce the production of CH 3 SH (methyl mercaptan) compound on halitosis-causing bacteria, Porphyromonas gingivalis. …”

“…Chlorhexidine is an antibacterial active ingredient belonging to the gold standard contained in mouthwash. 21 Chlorhexidine has a broad-spectrum antibacterial activity both against the Gram positive and Gram negative. 22 Antimicrobial activities of chlorhexidine are performed by damaging the cytoplasmic membrane.…”