In vitro antimicrobial effects of Myristica fragrans essential oil on foodborne pathogens and its influence on beef quality during refrigerated storage

Abstract

Nutmeg (Myristica fragrans) seed was subjected to the hydro‐distillation method to extract its essential oil (NEO). Its main constituents were α‐pinene (20.16%), sabinene (14.45%), and β‐pinene (13.26%) with great antimicrobial and antioxidant actions. A novel edible coating was then fabricated based on the sage seed mucilage (SSM) and NEO, to ameliorate the quality and shelf‐life of beef slices. The NEO‐loaded SSM coating was able to significantly decrease the population of total viable count, Escherichia coli, Staphylococcus aureus, psychrotrophic bacteria, and fungi. Moreover, lipid oxidation of beef slices was remarkably suppressed upon the application of bioactive NEO‐loaded SSM edible coating, as compared with the control by Day 6. The coated beef sample, especially NEO‐rich SSM coated ones perceived a higher consumer acceptance and firmness than the SSM coated and noncoated samples. The SSM edible coating containing NEO could therefore have the potential to control the growth of pathogenic microorganisms and lipid oxidation, and to improve the color stability and sensory properties of meat and meat products.

The impact of Qodume Shirazi seed mucilage‐based edible coating containing lavender essential oil on the quality enhancement and shelf life improvement of fresh ostrich meat: An experimental and modeling study

“…Quantification of LO components was performed using a Beifen 3420A gas chromatograph (Agilent Technologies 7890 A) equipped with a split injector, a flame ionization detector, and a DB‐5 capillary column (30 m × 0.25 mm, 0.25 μm stationary phase thickness). To identify the LO constituents, the obtained retention profile was compared with that of known samples already analyzed by a gas chromatograph (Agilent 7890A) coupled to a mass spectrometer (Agilent 5975C) with similar column and operating conditions (Alizadeh Behbahani & Imani Fooladi, 2018b; Kiarsi et al., 2020).…”

“…Scavenging activity was performed according to Kiarsi et al. (2020) method. For this purpose, 25 mg of LO was dissolved in 5 ml of distilled water; then, 0.1 ml of this solution was mixed with 3.9 ml of methanol DPPH solution (0.1 M methanol DPPH solution).…”

“…In recent years, successful studies have been conducted on the application of various types of polysaccharide edible coatings containing different essential oils in extending the shelf life of different types of meat. Among such coatings, Lallemantia iberica mucilage containing Cuminum cyminum essential oil (Alizadeh Behbahani et al., 2020), Salvia macrosiphon mucilage in combination with Myristica fragrans (Kiarsi et al., 2020), the mixture of Lepidium sativum and Heracleum lasiopetalum (Barzegar et al., 2020), Lallemantia royleana mucilage blended with Allium hirtifolium (Alizadeh Behbahani & Imani Fooladi, 2018a), and Plantago major mucilage combined with Anethum graveolens essential oil (Alizadeh Behbahani et al., 2017) could be pointed out.…”

Edible and Functionalized Films/Coatings—Performances and Perspectives

“…Usually by mixing different ingredients, the diffraction bands existing in diffractograms sustain some attenuation, diminishing, scattering, and broadening, which are all clear indications of the crystallinity lowering. [32,47,166,174,211,235,239,240] Antibacterial activity is performed by the assessment of the zone of inhibition assay on a solid medium placed in Petri dishes and containing specific media for each microorganism tested [74,125,129,141,171,176,179,187,213,238,[241][242][243][244][245][246][247][248][249][250][251][252][253][254][255][256][257][258][259].…”

“…Total flavonoids (aluminum chloride reagent) data are expressed in mg of quercetin equivalents for 100 g of film (mg QE/100 g film) [46,140,163,188,216,217,[229][230][231][232][233] • DPPH method (1,1-diphenyl-2-picrylhydrazyl radical reagent-DPPH) data are expressed in mg of ascorbic acid equivalents for 100 g of film (AAE/100 g of film) [30,32,37,47,72,160,162,163,169,199,[233][234][235][236][237][238][239] • ABTS (2,20-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid reagent-ABTS) data are expressed in mg of ascorbic acid equivalents for 100 g of film (AAE/100 g of film) [32,47,166,174,211,235,239,240] Antibacterial activity is performed by the assessment of the zone of inhibition assay on a solid medium placed in Petri dishes and containing specific media for each microorganism tested [74,125,129,141,171,176,179,…”