In vitro antimicrobial activity of mexican plants on bovine mastitis bacteria: preliminary studies

Alonso, Mariana Macías; Salazar, Julio C López; Osegueda-Robles, Soraya; Guerrero, Iván Córdova; García, Fernanda   Ledezma; Marrero, Joaquín González

doi:10.14393/bj-v36n1a2020-42137

Cited by 3 publications

(2 citation statements)

References 13 publications

(13 reference statements)

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“…In similar studies, the extract of various plants inhibited the growth of some microorganisms at different rations. Different plants possess different constituents in different concentrations, which account for differential antimicrobial effect as also suggested (Alonso et al, 2020;Yihune and Yemata, 2019;Bereksi et al, 2018).…”

Section: Antibacterial Evaluationmentioning

confidence: 92%

Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae)

et al. 2023

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Croton argyrophylloides Muell. Arg., from the Euphorbiaceae family, popularly known as marmeleiro prateado or sacatinga, is a plant from the Caatinga biome commonly found in Brazil’s northeastern region. The present study aimed to evaluate the antioxidant activity of the species. The phytochemical study was performed through qualitative analysis of chemical constituents and quantitative determination of the total phenol content through the Folin-Ciocalteu test. The qualitative and quantitative antioxidant tests were performed using the DPPH method (2.2 diphenyl-1-picryl hydrazil) and ferric reducing antioxidant power (FRAP). The minimum inhibitory concentration (MIC) was determined by microdilution in 96-well plates. The ethanolic extract of the leaves of C. argyrophylloides manifested antioxidant action in the quantitative DPPH test with a significant bioactivity of 84.70 AAO% in 500 µg/mL, with an EC50 of 236.79. The content of total phenolic compounds was 946.06 mg of gallic acid equivalents/g of sample, and total flavonoids was 58.11 mg of quercetin equivalents/g of sample, the result obtained for FRAP was 15294.44 µM Trolox/g of sample and ABTS was 718 μM Trolox of sample. The prospecting of the chemical constituents of the leaves of C. argyrophylloides revealed the presence of the main compounds that manifests the antioxidant activity and it was proven by the DPPH method that there is antioxidant activity in the analyzed sample, in addition to demonstrating a significant content of phenolic compounds and total flavonoid content in the species, which corroborates the antioxidant activity of the plant sample. The leaf extracts presented growth inhibition halos of 10 and 12 mm upon Staphylococcus aureus ATCC 25923.

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Section: Antibacterial Evaluationmentioning

confidence: 92%

Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae)

et al. 2023

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“…Mastitis is a complex disease caused by various bacterial pathogens, mainly Staphylococcus aureus , and Escherichia coli . It is also reported to be the most common reproductive disease in dairy cattle and a heavy burden disease in dairy farms worldwide (Alonso et al., 2020; Harjanti & Sambodho, 2020). It is believed that the bacterial infection in mastitis cases is related to the disruption of alveolar cell‐integrity, sloughing of cells, induced apoptosis and increase of poorly differentiated cells.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of in vitro antibacterial effect of essential oil and some herbal plant extract used against mastitis pathogens

Arbab

Ullah

Bano

et al. 2022

Veterinary Medicine & Sci

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Background Mastitis in dairy cattle is a highly prevalent infectious disease, caused by various pathogens, mainly Staphylococcu aureus and Escherichia Coli, considerable economic loss worldwide. Objectives The aim of this study was to evaluate the in vitro activity of Herbal plants used against S. aureus and E. coli bacteria which are the causative agents of mastitis. Methods Therefore, in this study we investigate the antimicrobial effect of plant to evaluate the in vitro antibacterial activity of squaw mint (Mentha pulegium L., Lamiaceae family), catnip (Nepeta cataria L., Lamiaceae), lemon balm (Melissa officinalis L., Lamiaceae), for mastitis treatment. Solutions prepared in fixed oils, against S. aureus and E. coli bacteria which are the main agents of mastitis. Isolation and antibiotic susceptibility analyses of milk samples taken from 100 subclinical mastitis dairy cows were performed. The antibacterial properties of the solutions were analysed by a disk diffusion method. Results In the bacterial isolation, S. aureus was determined 97.7% and E. coli 53.5% positive of cows with mastitis. Antibacterial susceptibility test of the Lemon balm extract and essential oil showed maximum zone of inhibition against S. aureus 30 µl (23 mm), followed by 20 µl (19 mm), E. coli (19 mm) and 10 µl (5–7 mm), of the same extract against the Gram‐positive bacteria. The ethanol extracts show the similar activity against the Gram‐negative bacteria at 30, 20, and 10 µl (18–20 mm). Followed by S. aureus, when the zone areas for the susceptible solutions (Lemon balm, and essential oil) and the control group were compared, determined that there was little difference between for S. aureus and E. coli. Conclusions This study hence indicated that in vitro cultured plantlets of lemon balm and peppermint oil can be used as the alternative method for production of mastitis and cheap source its precursor with antimicrobial activities.

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Mutactimycin AP, a New Mutactimycin Isolated from an Actinobacteria from the Atacama Desert

et al. 2022

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Bacteria belonging to the phylum Actinobacteria are a very good source of antibiotics, and indeed dominate the current clinical antibiotic space. This paper reports Mutactimycin AP, a new compound belonging to an anthracycline-type family of antibiotics, isolated from a Saccharothrix sp. This actinobacterial strain was isolated from the rhizosphere of lupine plants growing in the extreme hyper-arid Atacama Desert. Structural characterization was carried out using electrospray ionization-mass spectrometry (ESI-MS) and NMR spectroscopy in combination with molecular modelling. The compound was tested against the ESKAPE pathogens, where it showed activity against MRSA and five strains associated with bovine mastitis, where it showed activity against Enterococcus pseudoavium and Staphylycoccus Aureus subsp. Aureus.

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In vitro antimicrobial activity of mexican plants on bovine mastitis bacteria: preliminary studies

Cited by 3 publications

References 13 publications

Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae)

Phytochemical characterization, antioxidant potential and antibacterial activity of the Croton argyrophylloides Muell. Arg. (Euphorbiaceae)

Evaluation of in vitro antibacterial effect of essential oil and some herbal plant extract used against mastitis pathogens

Mutactimycin AP, a New Mutactimycin Isolated from an Actinobacteria from the Atacama Desert

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