In Vitro antimicrobial activity of medicinal plant Rumex Nervosus against selected oral pathogens

Abstract

Background: Rumex nervosus is a plant found and used in Saudi Arabia as traditional herbal medicine. Various types of plant extracts with inherent synergistic properties are being used against oral diseases. Thus, in the present study, the anti-microbial activity of Rumex nervosus extracts was studied against six common oral pathogenic bacterial strains and a pathogenic fungus. Materials and Method: Plants of R. nervosus were collected and air-dried, and extracts from various plant parts were obtained. Six clinical isolates of bacteria, namely Staphylococcus aureus, Streptococcus mutans, S. salivarius, S. sanguis, E. faecalis , and Lactobacillus acidophilus ), and one pathogenic Candida ( C. albicans ) were obtained. Antibacterial and antifungal activity of R. nervosus was determined using the Kirby–Bauer agar disc diffusion method. Zones of inhibition were recorded after 48 h of incubation. Data collected were analyzed. A two-way analysis of variance (ANOVA) was applied. P < 0.05 was considered statistically significant. Results: Methanol extract from leaves were highly effective against S. aureus , with a mean inhibition zone of 33 mm, followed by a 28-mm zone of inhibition using an extract from roots and a minimum inhibition zone using an extract from stems. Zones of inhibition using methanol extract from roots were effective against S. mutans , S. sanguinis , E. faecalis , and L. acidophilus , with mean inhibition zones being 19, 17, 33, and 31 mm, respectively. Conclusion: The study has provided insight into a new potential herbal anti-microbial agent that may benefit dental care.

Bone marrow mesenchymal stem cells response on collagen/hyaluronan/chondroitin scaffold enriched with gentamicin -loaded gelatin microparticles for skin tissue engineering

“…The activity of ColI-HA-CS-GS/GMs, ColI-HA-CS-GS and ColI-HA-CS scaffolds against E. coli and S. aureus in vitro was determined by inhibition zone method. 35 The scaffolds were added to the Luria Broth (LB) solid medium containing 200 μL S. aureus and E. coli (10 5 cfu/mL, determined at 600 nm using the UV-visible spectrophotometer) for 16 h at 30°C. 36 The relative antibacterial effect was measured the zone of inhibition (ZOI) with a digital caliper.…”

Effects of Rumex nervosus leaf powder supplementation on carcasses compositions, small intestine dimensions, breasts color quality, economic feasibility in broiler chickens

“…Some studies on the use of medicinal plants containing gallic acid and other bioactive derivatives in poultry feed show promising results in promoting growth performance, increasing feed efficiency, and improving carcass characteristics and meat quality ( Biswas et al, 2023 ; Vlaicu et al, 2023 ), whereas studies with Rumex nervosus are very limited. Rumex nervosus , as a traditional medicinal shrub belonging to the Polygonaceae family and distributed in Arab and African countries, contains gallic acid and has numerous biological activities ( Al-Sunafi, 2016 ; Aati et al, 2019 ; Gemechu et al, 2021 ; Al-Farhan et al, 2022 ; Al-Garadi et al, 2022 ; Boukhary et al, 2023 ). Regardless of extracts or powders, Rumex nervosus leaf powder ( RNLP ) have antioxidant, anti-inflammatory ( Al-Quraishy et al, 2020 ; Qasem et al, 2020 ), anticoccidial ( Qasem et al, 2020 ; Qaid et al, 2021b ), and antimicrobial properties ( Al-Garadi et al, 2022 ), as well as improves the productive performance of broilers ( Al-Quraishy et al, 2020 ; Azzam et al, 2020 ).…”