In vitro antimicrobial activities of methanolic extract from marine alga Enteromorpha intestinalis

Demirtaş, İbrahim; Lim, Sheh-Hong

doi:10.1016/j.apjtb.2015.07.012

Cited by 21 publications

(10 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Red algae G. persica and brown algae S. boveanum were collected from the Persian Gulf coast, Bushehr, Iran. The algae were washed with fresh water and kept in the shade (Ibrahim & Lim, ) for 5 to 7 days at 37–41°C until fully dried. The dried algae were crushed with mortar and pestle.…”

Section: Methodsmentioning

confidence: 99%

The effect of supplementing diets with extracts derived from three different species of macroalgae on growth, thermal stress resistance, antioxidant enzyme activities and skin colour of electric yellow cichlid (Labidochromis caeruleus )

et al. 2018

View full text Add to dashboard Cite

The purpose of this study was to evaluate the growth, thermal stress resistance, antioxidant enzyme activities and skin colour of Labidochromis caeruleus (electric yellow cichlid) fed the diets supplemented with extracts derived from brown macroalga Sargassum boveanum, red macroalga Gracilaria persica and green macroalga Entromorpha intestinalis. One hundred and forty‐four fish were randomly distributed into 12 tanks and subjected to cold‐shock stress after 8 weeks of feeding the diets containing 1,000 mg extracts of macroalgae. Supplementation of fish diet with algal extracts led to improved growth performance (including higher final weight and weight gain) when compared to the control group, which was fed the non‐supplemented diet. The survival rate after cold‐shock stress was significantly higher in those fish fed the diets containing macroalgal extracts, especially E. intestinalis extract (75%). The superoxide dismutase activity in all dietary treatments was significantly lower than control, whereas no significant difference in the activities of catalase and lysozyme was observed among treatments. In addition, inclusion of macroalgal extracts in the diet resulted in higher a* (redness) and b* (yellowness) values compared to the control group. These results suggest that macroalgal extracts, especially E. intestinalis extract, can be used as feed additive for increasing antioxidants capacities as well as enhancing pigmentation in electric yellow cichlid.

show abstract

Section: Methodsmentioning

confidence: 99%

The effect of supplementing diets with extracts derived from three different species of macroalgae on growth, thermal stress resistance, antioxidant enzyme activities and skin colour of electric yellow cichlid (Labidochromis caeruleus )

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Ulva intestinalis , a marine green algae belonging to the family of Ulvaceae, consisting of a tubular frond and unbranched thalli [19]. It is able to reproduce using unfused gametes, spores, and zygotes.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Identification of ACE Inhibitory Peptides from the Marine Macroalga Ulva intestinalis

Sun

et al. 2019

Marine Drugs

100

View full text Add to dashboard Cite

Angiotensin I-converting enzyme (ACE) inhibitory peptides derived from seaweed represent a potential source of new antihypertensive. The aim of this study was to isolate and purify ACE inhibitory peptides (ACEIPs) from the protein hydrolysate of the marine macroalga Ulva intestinalis. U. intestinalis protein was hydrolyzed by five different proteases (trypsin, pepsin, papain, α-chymotrypsin, alcalase) to prepare peptides; compared with other hydrolysates, the trypsin hydrolysates exhibited the highest ACE inhibitory activity. The hydrolysis conditions were further optimized by response surface methodology (RSM), and the optimum conditions were as follows: pH 8.4, temperature 28.5 °C, enzyme/protein ratio (E/S) 4.0%, substrate concentration 15 mg/mL, and enzymolysis time 5.0 h. After fractionation and purification by ultrafiltration, gel exclusion chromatography and reverse-phase high-performance liquid chromatography, two novel purified ACE inhibitors with IC50 values of 219.35 μM (0.183 mg/mL) and 236.85 μM (0.179 mg/mL) were obtained. The molecular mass and amino acid sequence of the ACE inhibitory peptides were identified as Phe-Gly-Met-Pro-Leu-Asp-Arg (FGMPLDR; MW 834.41 Da) and Met-Glu-Leu-Val-Leu-Arg (MELVLR; MW 759.43 Da) by ultra-performance liquid chromatography-tandem mass spectrometry. A molecular docking study revealed that the ACE inhibitory activities of the peptides were mainly attributable to the hydrogen bond and Zn(II) interactions between the peptides and ACE. The results of this study provide a theoretical basis for the high-valued application of U. intestinalis and the development of food-derived ACE inhibitory peptides.

show abstract

“…The antimicrobial activity of E. intestinalis methanolic extract was shown in the study of Ibrahim et al (2015), among the tested microorganisms, Staphylococcus aureus (MRSA) were susceptible to the extract and inhibited with the MIC value of above 6.25 mg/mL (Ibrahim et al, 2015).…”

Section: Ec Sf Camentioning

confidence: 99%

The Antimicrobial Activity of Enteromorpha sp. Methanolic Extract and Gelatin Film Solution Against on Some Pathogens

Eliuz¹,

Börekçi²,

Ayas³

2019

Marine Science and Technology Bulletin

View full text Add to dashboard Cite

Pathogenic microorganisms have been the primary cause of foodborne disease and food poisoning throughout the world for years. The use of natural antimicrobial agents in food coating has been effective in regulating the adverse effects of pathogens in food. Increasing antimicrobial efficacy in these coatings is one of the current issues of the food industry. In the present study, the antimicrobial properties of Enteromorpha sp., which is a marine algae, and gelatin film solution incorporated with Enteromorpha sp. methanol extract have been investigated. The contents of Enteromorpha sp. methanol extract were determined by Gas chromatography-mass spectrometry (GCMS). The most important components in the extract were methyl palmitoleate, neophytadiene, phytol, methyl linolenate and methyl stearate. The minimum inhibitory concentration (MIC; the lowest concentration of test material which results in 99.9% inhibition of growth) of Enteromorpha sp. on Escherichia coli, Staphylococcus aureus and Candida albicans were found to be between 10.79 mg/mL and 26.86 mg/mL by spectrophotometric microdilution technique. The antimicrobial effect of gelatin-Enteromorpha sp. methanolic extract film solution against the same pathogens was determined by disc diffusion method. The inhibition zone of gelatin-Enteromorpha sp. film solution was reported between 0.1 and 5.1 mm against pathogens. After a 24-h incubation, the effectiveness of the film solution was lower (1.3 mm) when compared to the extract on E. coli (5.1 mm). As a result, this study clearly showed that Enteromorpha sp. could be used as antimicrobial food coating agent, especially, in E. coli struggle.

show abstract

In vitro antimicrobial activities of methanolic extract from marine alga Enteromorpha intestinalis

Cited by 21 publications

References 6 publications

The effect of supplementing diets with extracts derived from three different species of macroalgae on growth, thermal stress resistance, antioxidant enzyme activities and skin colour of electric yellow cichlid (Labidochromis caeruleus )

The effect of supplementing diets with extracts derived from three different species of macroalgae on growth, thermal stress resistance, antioxidant enzyme activities and skin colour of electric yellow cichlid (Labidochromis caeruleus )

Preparation and Identification of ACE Inhibitory Peptides from the Marine Macroalga Ulva intestinalis

The Antimicrobial Activity of Enteromorpha sp. Methanolic Extract and Gelatin Film Solution Against on Some Pathogens

Contact Info

Product

Resources

About