In vitro antimicrobial activities of several extracts endophytic Pseudomonas azotoformans UICC B-91

Abstract

Pseudomonas azotoformans known for the benefit on agriculture area as soil fertility enhancer. Pseudomonas azotoformans UICC B-91 was successfully isolated from tissues of N. altissima which traditionally known for treating infectious diseases. Therefore, endophytic P. azotoformans might contain antimicrobial compound. This research aims on exploring the antimicrobial activities of several extracts P. azotoformans UICC B-91 against pathogenic microbes. Isolates were extracted with dichloromethane, chloroform, and ethyl acetate. Each crude extract was observed for its potential antimicrobial activity with disc diffusion method against several pathogen microbes. Bioactive compound was assayed by thin layer chromatography (TLC) and screened by bioautography. Results indicated that all crude extract have inhibitory effect on Escherichia coli ATCC 8739, Bacillus cereus ATCC 10876, Staphylococcus aureus ATCC 6583, Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 25241, Pseudomonas aeruginosa ATCC 15442, Bacillus subtilis ATCC 19659 and Candida albicans ATCC 10231. Highest clear zone was on dichloromethane extract. TLC fraction of dichloromethane extract was able to inhibit growth on pathogenic bacteria (except for B. subtilis ATCC 19659 and S. aureus ATCC 6583) and fungi at minimum concentration in the range 125-1,000 μg/mL and 125 μg/mL, respectively. Bioactive compound from endophytic P. azotoformans extracted with dichloromethane have antibacterial and anticandidal effect.

Antimicrobial Compound from Endophytic Pseudomonas azotoformans UICC B-91 of Neesia altissima (Malvaceae)

“…20 F1 also has strong anticandidal activity than its antibacterial activity. 4 Therefore, this identification compound supported that F1 has a potential as anticandidal compound.…”

“…18,27 From our previous research, F3 also have antibacterial and anticandidal activity. 4 The interesting study was reported on cytotoxic activity of sarmentine, but indeed its activity was still lower than taxol as positive control. 17 From our observation, there hasn't any publication on sarmentine from Pseudomonaceae.…”

“…F1 has shown antimicrobial activity on the pathogenic microbes-on previous research. 4 Futoamide (1) containing benzene ring attached with dioxol (benzodioxole), heptadiene as polyunsaturated fatty acid, and isobutyl amide. Grouped as alkaloamide or isobutyl amide alkaloid syntheses from L-lysine, futoamide was also found on family Piperaceae, with bioactive activity as antioxidant.…”

“…3 Previous research has been observed the antimicrobial activity from centrifugated extract of endophytic P. azotoformans, against several bacteria and C. albicans. 4 with notice result on its anticandidal activity. For further observation, the antimicrobial compounds were identified by mass spectrophotometry.…”

Antifungal effect of Pseudomonas azotoformans UICC B-91 metabolites on hyphal growth of Trichophyton mentagrophytes

“…Isolation and extraction of the bioactive compounds from P. azotoformans UICC B-91 was carried out following the method of Oktarina et al [13] with modifications. The starter was prepared by inoculating one dose of P. azotoformans UICC B-91 from NA medium obliquely into 20 mL RB in a 150 mL Erlenmeyer flask, followed by incubation in an incubator shaker for 24 hours at 30 °C at 100 rpm.…”