In vitro antileishmanial potentialities of essential oils from Citrus limon and Pistacia lentiscus harvested in Tunisia

Maaroufi, Zeineb; Cojean, Sandrine; Loiseau, Philippe M.; Yahyaoui, M.; Agnely, Florence; Abderraba, Manef; Mekhloufi, Ghozlène

doi:10.1007/s00436-020-06952-5

Cited by 18 publications

(11 citation statements)

References 58 publications

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“… 29 , 30 Numerous plant and herbal extracts and oils have been used as antiparasitic agents against liver flukes, tapeworm, and protozoan. 31 − 34 N. sativa oil has been shown in several studies to have antibacterial, antifungal, and antiviral properties. 9 The antibacterial activities are due to the presence of the major component thymoquinone in the N. sativa oil.…”

Section: Discussionmentioning

confidence: 99%

“…Plant-derived natural products have been central to drug discoveries for both antibiotics and antiparasitic drugs. Switching to alternate therapies, particularly plant and other natural product-derived drugs, may advance the search for better, more cost-effective, and safe antileishmanial agents. , Numerous plant and herbal extracts and oils have been used as antiparasitic agents against liver flukes, tapeworm, and protozoan. − N. sativa oil has been shown in several studies to have antibacterial, antifungal, and antiviral properties .…”

Section: Discussionmentioning

confidence: 99%

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Thymoquinone Induced Leishmanicidal Effect via Programmed Cell Death inLeishmania donovani

et al. 2022

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Visceral leishmaniasis (VL) or kala-azar is a vector-borne dreaded protozoal infection that is caused by the parasite Leishmania donovani . With increases in the dramatic infection rates, present drug toxicity, resistance, and the absence of an approved vaccine, the development of new antileishmanial compounds from plant sources remains the keystone for the control of visceral leishmaniasis. In this study, we evaluated the leishmanicidal effect of thymoquinone against L. donovani with an in vitro and ex vivo model. Thymoquinone exhibited potent antipromastigote activity with IC 50 and IC 90 concentrations achieved at 6.33 ± 1.21 and 20.71 ± 2.15 μM, respectively, whereas the IC 50 and IC 90 concentrations were found to be 7.83 ± 1.65 and 27.25 ± 2.20 μM against the intramacrophagic form of amastigotes, respectively. Morphological changes in promastigotes and growth reversibility study following treatment confirmed the leishmanicidal effect of thymoquinone. Further, thymoquinone exhibited leishmanicidal activities against L. donovani promastigote through cytoplasmic shrinkage, membrane blebbing, chromatin condensation, cellular and nuclear shrinkage, and DNA fragmentation, as observed under scanning and transmission electron microscopy analyses. The antileishmanial activity was exerted via programmed cell death as proved by exposure of phosphatidylserine, DNA nicking by TUNEL assay, and loss of mitochondrial membrane potential. Thymoquinone at a concentration of 200 μM was devoid of any cytotoxic effects against mammalian macrophage cells. Thymoquinone showed strong leishmanicidal activity against L. donovani , which is mediated via an apoptosis mode of parasitic cell death, and accordingly, thymoquinone may be the source of a new lead molecule for the cure of VL.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Thymoquinone Induced Leishmanicidal Effect via Programmed Cell Death inLeishmania donovani

et al. 2022

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“…Pistacia türleri arasında P. lentiscus farklı bölgelerde kullanımı en yaygın olan ve reçinesi de 5000 yıldan beri kullanmakta olan bir tür olarak bilinmektedir [2]. Çalışma sonucunda uçucu yağın, makrofajlara karşı toksik etki ve antileishmanial aktivite sergilediği görülmüştür [70]. Oliveira ve ark., pek çok bitki ekstresinin in vitro antihelmintik aktivitesini inceledikleri çalışmada, Yumurta Kuluçka İnhibisyon Testi ile antihelmentik etkiden kısmen de olsa polifenollerin sorumlu olabileceklerini göstermişlerdir.…”

Section: Etnofarmakolojik Kullanımlarıunclassified

Pistacia Lentiscus L. Türünün Botani̇k, Etnofarmakoloji̇k, Fi̇toki̇myasal Ve Farmakoloji̇k Akti̇vi̇teleri̇ni̇n Değerlendi̇ri̇lmesi̇

Pekacar¹,

Orhan²

2022

Ankara Universitesi Eczacilik Fakultesi Dergisi

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Amaç: Pistacia, içerisinde P. lentiscus gibi tıbbi türleri ihtiva eden Anacardiaceae familyasına ait, Akdeniz havzası boyunca yaygın dağılıma sahip bir cinstir. P. lentiscus'un çeşitli kısımları geleneksel tıpta pek çok hastalığın tedavisinde kullanılmaktadır. Bu derlemede, P. lentiscus türünün botanik özellikleri, geleneksel kullanımları, fitokimyasal içeriği ve farmakolojik aktiviteleri ile ilgili kapsamlı bilgiler özetlenmeye çalışılmıştır.Sonuç ve Tartışma: Literatür taramaları bu türün antioksidan, antimutajenik, animikrobiyal, antiviral, antienflamatuvar, gastrointestinal hastalıklar, karaciğer ve serum biyokimyasal parametreler üzerindeki etkileri, antiaterosklerotik ve antidiyabetik, antiparaziter, antitümör, antikolinesteraz, nöroprotektif ve yara iyileştirici aktivitelerin de olduğu çok çeşitli farmakolojik aktiviteler bakımından değerlendirildiğini göstermiştir. P. lentiscus terpenoitler, fenolik bileşikler, yağ asitleri ve steroller gibi çeşitli fitokimyasal bileşenleri ihtiva etmektedir. Yapılan araştırma sonucunda P. lentiscus türünün çeşitli farmasötik şekillerde doğal kaynaklı ilaçların geliştirilmesi ve ticarileştirilmesi için önemli bir kaynak olduğu, ama risk faktörlerini daha iyi aydınlatmak adına daha fazla preklinik ve klinik çalışmaya gereksinim duyulduğu sonucuna varılmıştır.

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“…As well as [50] they were extracted peel essential oil of C. limon and analyzed by GC/MS technical to identified a compounds that was limonene (55.40 %) and neral (10.39 %) were found as major compound followed by trans-verbenol (6.43 %) and decanal (3.25 %), also a study was conducted on Citrus limon grown in Iraq by [52] to appear chemical composition and biological properties of the essential oil that extracted by hydro-distillation method and analyzed by GC-MS, a results demonstrated that major constituents of essential oil of C. limon peels were limonene (29.52 %), β-pinene (23.89 %), citronellal (11.53 %) and thymol (9.79 %). Gas Chromatography Mass Spectroscopy, quick and reliable platform for essential oil analysis in both qualification and quantification [40] ,it is a hyphenated system is a very compatible technique and the most commonly used technique for the identification and quantification purpose [106] ,It is a tool with good selectivity and high sensitivity is necessary for natural essential oil because they are usually composed of many different ingredients [40] .…”

Section: Isolation and Diagnosis Of Limonene Compound From Terpenoid ...mentioning

confidence: 99%

Evaluates the active substances of extracts of date vinegar and Citrus lemon plant fruit on Enterobius vermicularis worm in vitro

Al-Taei

Al-Quraishi

2022

ijhs

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This work was conduct to account the effects of cold and hot aqueous extracts of Iraqi Citrus limon plant fruits and date vinegar in paralysis and die of Enterobius vermicularis parasite in vitro and determine the most influential plant for extracted secondary compound (alkaloid, terpenoids and phenolic compounds) from it and then determine a most influential secondary compound for analysis by Gas chromatography /mass spectroscopy technical (GC/MS) and extract the most concentrated compound from it to test in paralyze and kill worms. The results appear that cold aqueous extract of Citrus limon plant fruits have a high influential especially by 300 mg/ml concentration where its average arithmetic for paralysis and die of worms equal 29.00 and 57.33 minute , respectively, followed by hot aqueous extract of Citrus limon plant fruits has effect on paralysis and die of worms in 300 mg/ml concentration where its led to paralysis and die of worms at 67.33 and 104.00 minute , followed by cold aqueous extract of date viengar extract where its average arithmetic for paralysis and die of worms in 300 mg/ml concentration equal 110.00 and 193.00 minute.

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In vitro antileishmanial potentialities of essential oils from Citrus limon and Pistacia lentiscus harvested in Tunisia

Cited by 18 publications

References 58 publications

Thymoquinone Induced Leishmanicidal Effect via Programmed Cell Death inLeishmania donovani

Thymoquinone Induced Leishmanicidal Effect via Programmed Cell Death inLeishmania donovani

Pistacia Lentiscus L. Türünün Botani̇k, Etnofarmakoloji̇k, Fi̇toki̇myasal Ve Farmakoloji̇k Akti̇vi̇teleri̇ni̇n Değerlendi̇ri̇lmesi̇

Evaluates the active substances of extracts of date vinegar and Citrus lemon plant fruit on Enterobius vermicularis worm in vitro

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