In vitro antileishmanial and cytotoxicity activities of essential oils from Haplophyllum tuberculatum A. Juss leaves, stems and aerial parts

Abstract

BackgroundPlants used for traditional medicine produce diverse and complex secondary metabolites exhibiting various medicinal properties. The medicinal plant Haplophyllum tuberculatum is used by native people against malaria and parasitic infections.MethodsIn this study and in order to contribute for the search of new natural drugs for leishmaniasis, the essential oils of H. tuberculatum leaves, stems and aerial parts (leaves+stems) collected in two different periods, 2013 and 2015, and their components by GC/FID and GC/MS analyses were investigated. Those collected in 2013 were also re-analyzed two years later. The extracted oils were screened in vitro for anti-leishmanial activity on Leishmania mexicana mexicana (L.m.m.) promastigotes and cytotoxicity on the Chinese Hamster Ovary (CHO) cell line. Limonene (1.5 – 8%), its isomers (R- (+)-limonene and S-(-)-limonene), linalool and octanol were also tested.ResultsResults showed that the chemical composition varied according to the year of collection. Though major compounds remain almost the same, qualitative and quantitative variations in the composition of the EOs can be observed between the two years of collection, with some minor compounds identified only in one type of samples. Variation in the composition were also observed in the re-analyzed volatile oils, showing stability concerns. The essential oils and R-(+)-limonene showed moderate anti-leishmanial activity. Their IC50 range from 6.48 to 50.28 μg/ml. Cytotoxicity assays for theses volatile extracts, R- (+)-limonene and S- (-)-limonene on CHO cells showed relatively potent cytotoxicity with a selectivity index <10. Their CC50 range from 27.79 to 82.56 μg/ml.ConclusionsThe findings of the present study demonstrated that H. tuberculatum might not be considered as a natural source for production of new anti-leishmanial agents without further analyzing its eventual in vivo toxicity as well as that of major pure compounds.

Essential Oil of Deverra tortuosa Aerial Parts: Detailed Chemical Profile, Allelopathic, Antimicrobial, and Antioxidant Activities

“…sihthotpiana , E. tournefortii , E. orientalis , E. chrysantha , and E. lamondiana [35] . Piperitol was also very common compound in EOs of some plant species such as Mentha longifolia , [36] Cymbopogon schoenanthus , [37] and Haplophyllum tuberculatum [38] . The multitude of the monoterpene hydrocarbon compounds, terpinene, and cymene derivatives, were already reported in the EO of the two samples of D. tortuosa collected from Wadi Hagul and west Alexandria, Egypt [31] .…”

Haplophyllum buxbaumii ekstresinin skuamöz hücreli karsinoma üzerine apoptotik etkileri

“…These results suggest that HP has toxicity on cancer cells while protecting the normal ones. Previous studies on HP have shown that its essential oils have potentially protective effects on oxidative stress, highly cleaning efficacy and protective effects on human stroma U373-MG cells against H2O2 damage 15,16 . It has been proven that essential oils inhibit ROS production caused by H2O2.…”

Chemical Composition, Antioxidant Potential and Antimicrobial Properties of the Essential Oils of Haplophyllum tuberculatum (Forsskal) A. Juss from Morocco

“…The characteristic of the oils studied is its richness in sesquiterpenes, the thing which makes it different from other essential oils studied in other regions which are in their majority rich in oxygenated monoterpenes and monoterpene hydrocarbons. Thus, studies conducted on H. tuberculatum collected in Tunisia: 33 they showed that the essential oils of this plant was rich in monoterpenoids (20.7% for monoterpenic hydrocarbons, 46.7% for oxygenated monoterpenes and 0. 5% for sesquiterpene hydrocarbons), while on the other hand the study showed the richness of the essential oils of this plant in trans-p-menth-2-en-1-ol (18.23%), β-phellandrene (14.46%), piperitone (14.33%), cis-p-menth-2-en-1-ol ( 13.66%) and α-phellandrene (4.36%).…”