In vitro Antileishmanial Activity of Extracts from Endemic Moroccan Medicinal Plant Salvia verbenaca (L.) Briq. ssp verbenaca Maire (S. clandestina Batt. non L)

Abstract

Aims:The aim of this study was to evaluate in vitro the antileishmanial activities of organic extracts (methanol, n-hexane and dichloromethane extract) from Salvia clandestina (Lamiaceae) used in Moroccan medicinal plant. Study Design: Evaluation of in vitro antileishmanial activity of extracts and determination phenolic contents.

Phenolic extracts of Centaurium erythraea with novel antiradical, antibacterial and antileishmanial activities

“…It's the first time that the antileishmanial properties of C. erythraea extracts have tested against Leishmania promastigote forms. Our results are important compared with others tested extracts against the same species [13,35].…”

“…Several approaches have been investigated to search now molecules that have antimicrobial and antioxidant effects. Medicinal plants constitute a key candidate for screened natural compounds that possess antibacterial [12,19], antioxidant [11,13] and antileishmanial activities [35,37].…”

“…These three species were isolated and identified from Moroccan infected patients at the National Reference Laboratory of Leishmaniasis, National Institute of Health, Rabat-Morocco. The species were cultivated as described by Et-Touys et al [35]. Briefly, parasite cultures of each Leishmania species were washed with phosphate buffered saline (PBS) and centrifuged at 1 500 r/min for 10 min.…”

“…When cellular density reached a threshold concentration of 10 6 cells/mL, L. infantum, L. tropica and L. major promastigotes were washed twice with PBS and centrifuged at 2 500 r/min for 10 min. To evaluate the anti-promastigote activity, 100 μL of parasites culture was resuspended in a 96-well tissue culture plate, in fresh culture medium according to Et-Touys et al [35]. Briefly, parasites were incubated at 2.5 × 10 6 cells/well for 72 h at 23 °C in the presence of various concentrations of extract (μg/mL) dissolved in 1% DMSO.…”

“…Cell viability was also evaluated by determination of the extracts concentration which inhibited half of the cell population (IC 50 ), obtained by modeling by plotting percentage of inhibition versus concentration of extract using Original Program. Inhibition percentage (I) was calculated using the following formula [35]: I (%) = 100 × (Absorbance untreated cells -Absorbance treated cells)/Absorbance untreated cells.…”