In vitro antileishmanial activity of Anacardium othonianum and isolated compounds against Leishmania amazonensis

Abstract

This study analyzes the antileishmanial activity of the crude ethanol extract, fractions, and isolated compounds of A. othonianum nuts. Antileishmanial activity was evaluated against Leishmania amazonensis promastigotes in vitro. The phytochemical study was performed by high-performance liquid chromatography-high-resolution mass spectrometry-diode array detector (HPLC-HRMS-DAD) and by preparative HPLC. HPLC-HRMS-DAD analysis of the bioactive extract confirmed the presence of ten alkyl phenol derivatives that had previously been isolated from A. occidentale. Bioassay-guided isolation afforded cardanol triene, cardanol diene, cardanol monoene, cardol triene, anacardic acid triene, anacardic acid diene, and anacardic acid monoene. Cardol triene gave an IC50 of 80.66 µM. The obtained data suggest that the evaluated extract, fractions, and cardol triene had moderate activity against L. amazonensis promastigotes. This is the first description of alkyl phenols in A. othonianum.

HPLC method for quantifying verbascoside in Stizophyllum perforatum and assessment of verbascoside acute toxicity and antileishmanial activity

“…The antileishmanial activity was evaluated against L. amazonensis (IFLA/BR/67/PH8) promastigotes following a previously established reference ( Alvarenga et al., 2021 ). For this purpose, L. amazonensis promastigotes maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic (10,000 IU/mL penicillin and 10,000 mg/mL streptomycin) (Cultilab) were transferred to a 96-well plate (1 × 10 6 ).…”

O Gênero Anacardium: Composição Química E Propriedades Biológicas

“…O extrato bruto etanólico dos frutos e frações apresentaram atividade moderada contra as formas promastigotas de Leishmania amazonensis, o cardol trieno, dentre as substâncias isoladas no estudo, forneceu uma concentração inibitória IC 50 de 80,66 μM (ALVARENGA et al, 2021).…”