In vitro antihepatocellular carcinoma activity of secondary metabolites of Centaurea kilaea Boiss.

Abstract

The aim of this study was to investigate in vitro cytotoxic effects of previously isolated compounds (taraxasterol, salvigenin, 3'-O-methyleupatorin, oleanolic acid, jaceosidin and pectolinarigenin) from Centaurea kilaea chloroform extract on hepatocellular carcinoma cell lines, HepG2 and Hep3B, as well as to evaluate the effect on the normal cell line, NIH3T3. In vitro antihepatocellular carcinoma activity of compounds was assessed by MTT method. All compounds except pectolinarigenin caused more inhibition on HepG2 rather than Hep3B. Among these compounds, it was found that Jaseosidin had the highest anticancer activity with IC50 values of 137.66 µg/mL and 147.66 µg/mL on the HepG2 and Hep3B cell lines, respectively. 3'-O-methyleupatorin showed the second highest cytotoxicity with IC50 values of 151.98 µg/mL and 159.24 µg/mL against the HepG2 and Hep3B cell lines, respectively. The results indicated that Jaseosidin and 3'-O-methyleupatorin, had the best antiproliferative activity against hepatocellular carcinoma cell lines. Also, according to our best knowledge, this study is first report on antihepatocellular carcinoma activity of Jaseosidin and 3'-O-methyleupatorin.

Stevia Genus: Phytochemistry and Biological Activities Update

“…The compound afforded IC 50 of 32 and 40 µM on PC3 and B16-F10, respectively, not being active against MCF7. Most recently, in 2020, Şekerler [ 222 ] studied the effect of compound 92 in hepatocellular carcinoma cell lines, HepG2 and Hep3B, as well as in a normal cell line, NIH3T3. The in vitro antihepatocellular carcinoma activity of the compound was assessed by the MTT method.…”

HPLC analysis, Phytochemical Content, and Biological Effects of Centaurea kilae Against Some Metabolic Enzymes: In Vitro and In Silico Studies

“…The majority of substances used as drugs in the treatment of various diseases are isolated from plants or synthesized by deriving from natural molecules. Therefore, the chemical ingredients of the various plants and their enzyme inhibitor potentials are being screened to associate the herbal effect with the enzyme activities of medical importance [3–6] …”

Feature-Based Molecular Networking for the Exploration of the Metabolome Diversity of Common Egyptian Centaurea Species in Relation to Their Cytotoxic Activity

“…The following supporting information can be downloaded at: , Supplementary Figure S1: Overlaid base peak chromatogram of the studied Centaurea species in the positive ionization mode; Supplementary Table S1: Compound assignment of the studied Centaurea species extracts as revealed by UPLC-HRMS/MS analysis. Reference Citations of [ 11 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , …”