In vitro antifungal activity of antifungalmycin 702, a new polyene macrolide antibiotic, against the rice blast fungus Magnaporthe grisea

Xiong, Zhiqiang; Tu, Xiao-Rong; Wei, Sai-Jin; Huang, Lin; Li, Xunhang; Lü, Hui; Guo-quan, TU

doi:10.1007/s10529-013-1229-z

Cited by 24 publications

(13 citation statements)

References 14 publications

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“…These distorted hyphae had a flattened appearance. Similar conclusions have also been made in previous studies by Maoz and Neeman [25], and Xiong et al [26], who also found that hyphae morphology becomes irregular under certain treatment conditions.…”

Section: Resultssupporting

confidence: 91%

“…These phenomena that were observed agree with Arlorio et al [27], who observed the rupture of fungal cell walls upon exposure to citral. These phenomena are likely due to the structure of citral, because the effects of solutes on cell walls might be influenced by compounds that possess a hydrophilic group and an electron acceptor group, such as an aldehyde group [25,26].…”

Section: Resultsmentioning

confidence: 99%

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The Natural Product Citral Can Cause Significant Damage to the Hyphal Cell Walls of Magnaporthe grisea

Yin

et al. 2014

Molecules

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Abstract:In order to find a natural alternative to the synthetic fungicides currently used against the devastating rice blast fungus, Magnaporthe grisea, this study explored the antifungal potential of citral and its mechanism of action. It was found that citral not only inhibited hyphal growth of M. grisea, but also caused a series of marked hyphal morphological and structural alterations. Specifically, citral was tested for antifungal activity against M. grisea in vitro and was found to significantly inhibit colony development and mycelial growth with IC 50 and IC 90 values of 40.71 and 203.75 μg/mL, respectively. Furthermore, citral reduced spore germination and germ tube length in a concentration-dependent manner. Following exposure to citral, the hyphal cell surface became wrinkled with folds and cell breakage that were observed under scanning electron microscopy (SEM). There was damage to hyphal cell walls and membrane structures, loss of villous-like material outside of the cell wall, thinning of the cell wall, and discontinuities formed in the cell membrane following treatment based on transmission electron microscopy (TEM). This increase in chitinase activity both supports the morphological changes seen in the hyphae, and also suggests a mechanism of action. In conclusion, citral OPEN ACCESSMolecules 2014, 19 10280 has strong antifungal properties, and treatment with this compound is capable of causing significant damage to the hyphal cell walls of M. grisea.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

The Natural Product Citral Can Cause Significant Damage to the Hyphal Cell Walls of Magnaporthe grisea

Yin

et al. 2014

Molecules

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“…The genus Streptomyces strains have antagonistic activity against wide range of phytopathogens. Concerning rice blast, S. canus BYB02, S. padanus JAU4234 and S. sindeneusis 263 strongly inhibited M. oryzae . The antifungal activity of S. endus was only reported in potato and zinnia with low efficiency .…”

Section: Discussionmentioning

confidence: 98%

“…MJM4426 showed biocontrol potential on rice bacterial blight . Antifungalmycin 702, a new polyene macrolide antibiotic from Streptomyces padanus JAU4234, suppressed rice blast pathogen . Resistomycin from Streptomyces canus BYB02 possessed significant preventive efficacy against rice blast .…”

Section: Discussionmentioning

confidence: 99%

Isolation and evaluation of endophytic Streptomyces endusOsiSh‐2 with potential application for biocontrol of rice blast disease

Zeng

et al. 2016

J Sci Food Agric

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“…Similarly, Li et al ( 2014 ) observed that the natural product citral caused damage to hyphal cell walls and membrane structures of P. grisea . The cell walls of P. grisea are strongly influenced by hydrophilic and aldehyde groups which damage or rupture cell walls and membranes (Xiong et al, 2013 ). A few studies also have shown that 2,4-DAPG plays an important role in the control of phytopathogens by damaging hyphal tips, causing vacuolization and cell content disintegration, and disrupting multiple basic cellular pathways including membrane function, reactive oxygen regulation, and cell homeostasis (de Souza et al, 2003 ; Kwak et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Potential of Finger Millet Indigenous Rhizobacterium Pseudomonas sp. MSSRFD41 in Blast Disease Management—Growth Promotion and Compatibility With the Resident Rhizomicrobiome

et al. 2018

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Finger millet [Eleusine coracona (L). Gaertner] “Ragi” is a nutri-cereal with potential health benefits, and is utilized solely for human consumption in semi-arid regions of Asia and Africa. It is highly vulnerable to blast disease caused by Pyricularia grisea, resulting in 50–100% yield loss. Chemical fungicides are used for the management of blast disease, but with great safety concern. Alternatively, bioinoculants are widely used in promoting seedling efficiency, plant biomass, and disease control. Little is known about the impact of introduced indigenous beneficial rhizobacteria on the rhizosphere microbiota and growth promotion in finger millet. Strain MSSRFD41 exhibited a 22.35 mm zone of inhibition against P. grisea, produces antifungal metabolites, siderophores, hydrolytic enzymes, and IAA, and solubilizes phosphate. Environmental SEM analysis indicated the potential of MSSRFD41 to inhibit the growth of P. grisea by affecting cellular functions, which caused deformation in fungal hyphae. Bioprimed finger millet seeds exhibited significantly higher levels of germination, seedling vigor index, and enhanced shoot and root length compared to control seeds. Cross streaking and RAPD analysis showed that MSSRFD41 is compatible with different groups of rhizobacteria and survived in the rhizosphere. In addition, PLFA analysis revealed no significant difference in microbial biomass between the treated and control rhizosphere samples. Field trials showed that MSSRFD41 treatment significantly reduced blast infestation and enhanced plant growth compared to other treatments. A liquid formulated MSSRFD41 product maintained shelf life at an average of 108 CFU ml−1 over 150 days of storage at 25°C. Overall, results from this study demonstrated that Pseudomonas sp. MSSRFD41, an indigenous rhizobacterial strain, is an alternative, effective, and sustainable resource for the management of P. grisea infestation and growth promotion of finger millet.

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In vitro antifungal activity of antifungalmycin 702, a new polyene macrolide antibiotic, against the rice blast fungus Magnaporthe grisea

Cited by 24 publications

References 14 publications

The Natural Product Citral Can Cause Significant Damage to the Hyphal Cell Walls of Magnaporthe grisea

The Natural Product Citral Can Cause Significant Damage to the Hyphal Cell Walls of Magnaporthe grisea

Isolation and evaluation of endophytic Streptomyces endusOsiSh‐2 with potential application for biocontrol of rice blast disease

Potential of Finger Millet Indigenous Rhizobacterium Pseudomonas sp. MSSRFD41 in Blast Disease Management—Growth Promotion and Compatibility With the Resident Rhizomicrobiome

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