In Vitro Antifungal Activity of Silver Nanoparticles Biosynthesized with Beech Bark Extract

Mare, Anca Delia; Ciurea, Cristina Nicoleta; Man, Adrian; Mareș, Mihai; Toma, Felicia; Berța, Lavinia; Tănase, Corneliu

doi:10.3390/plants10102153

Cited by 27 publications

(14 citation statements)

References 61 publications

(86 reference statements)

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“…Similarly, the biosynthesized AgNPs using the seed extract of Syzygium cumini displayed anti-candidal activity at 0.125-0.250 mg/mL [42]. AgNPs biosynthesized by beech bark extract showed MIC of 0.03 mg/mL against C. albicans [43]. Furthermore, AgNPs synthesized by Caesalpinia ferrea extract displayed MIC values in the range of 156.25-1,250 µg/mL against C. albicans, C. glabrata, C. kruzei, and C. guilliermondii [44].…”

Section: Discussionmentioning

confidence: 96%

Therapeutic Effect of Green Synthesized Silver Nanoparticles Using Erodium glaucophyllum Extract against Oral Candidiasis: In Vitro and In Vivo Study

Abdallah

Ali

2022

Molecules

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Oral candidiasis (OC) is a fungal infection caused by an opportunistic fungi Candida albicans, which is found in the normal flora of healthy people. In this study, we examined the anti-candidal effect of green synthesized silver nanoparticles using leaf extract of Erodium glaucophyllum (EG-AgNPs) against C. albicans in vitro and in vivo. EG-AgNPs were synthesized for the first time using E. glaucophyllum extract and characterized by imaging (transmission electron microscopy (TEM), UV-VIS spectroscopy, zeta potential, X-ray diffraction (XRD), Energy dispersive x-ray analysis (EDX), and Fourier transform infrared spectroscopy (FTIR). A mouse model of OC was used for in vivo study. The agar well diffusion method showed the anti-candidal activity of EG-AgNPs against C. albicans with MIC 50 µg/mL. EG-AgNPs inhibited the dimorphic transition of C. albicans and suppressed the formation of biofilm by 56.36% and 52%, respectively. Additionally, EG-AgNPs significantly inhibited the production of phospholipases and proteinases by 30% and 45%, respectively. EG-AgNPs cause cytoplasm disintegration and deterioration of cell wall as imaged by SEM and TEM. Interestingly, EG-AgNPs did not display any cytotoxicity on the human gingival fibroblast-1 HGF-1 cell line at MIC concentrations. Topical treatment of the tongue of the OC mouse model with EG-AgNPs showed significant reduction in candidal tissue invasion, less inflammatory changes, and no tissue modification, in association with marked low scare and hyphal counts as compared to control group. In conclusion, our data demonstrated the potent inhibitory action of EG-AgNPs on the growth and morphogenesis of C. albicans in vitro and in vivo. Thus, EG-AgNPs represent a novel plausible therapeutic approach for treatment of OC.

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Section: Discussionmentioning

confidence: 96%

Therapeutic Effect of Green Synthesized Silver Nanoparticles Using Erodium glaucophyllum Extract against Oral Candidiasis: In Vitro and In Vivo Study

Abdallah

Ali

2022

Molecules

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“…The lyophilized IL-6 was reconstituted, according to the manufacturer's instructions, to achieve in vitro physiological final value of 250 pg/ml, comparable with achievable human serum concentrations during infections (range between 0 and 552.6 pg/ml) [10]. The experiments were performed in triplicate, based on previously published protocols [11,12]. As follows, the used methods are described shortly.…”

Section: Methodsmentioning

confidence: 99%

Mediation of Candida species growth and virulence by the proinflammatory cytokine IL-6

Ciurea

Kosovski

Toma

et al. 2021

Acta Marisiensis - Seria Medica

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Interleukin-6 (IL-6) is a cytokine with pleiotropic effects that might also influence the virulence traits of some microorganisms, but its direct influence over Candida spp. is currently unknown. The objective of the study is to determine the influence of IL-6 (250 pg/ml) on the growth rate and biofilm formation of C. albicans, C. parapsilosis, C. krusei, C. auris, and C. guilliermondii, as well as to analyze the influence of this citokine on the expression of three virulence genes (ALS3, HSP70, SAP2), respectively on the germ tube formation ability of C. albicans. The influence of IL-6 on growth rate was assessed by incubating the fungal cells in presence of IL-6 for 48 hours and assessing the optical density of the samples at five timepoints. The biofilm production in presence of IL-6 was studied in microtiter plates, using crystal-violet assay, the intensity of biofilms being evaluated by spectrophotometry. The expression of ALS3, HSP70, and SAP2 in C. albicans was studied by RT-PCR, reported to ACT1 housekeeping gene. The germ-tube test was performed to assess the influence of IL-6 on the filamentation rate of C. albicans. All test results were normalized against control, without added IL-6. The results showed that IL-6 influences the growth rate of C. albicans, C. parapsilosis, C. krusei, C. auris, and C. guilliermondii in a time-dependent way. Also, IL-6 inhibited the formation of biofilms for C. albicans and C. guilliermondii. In C. albicans cells, IL-6 induced upregulation of ALS3 and HSP70, while it down-regulated the SAP2 gene. IL-6 did not influence the germ-tube formation in C. albicans. In conclusion, IL-6 might exert, in vitro, direct effects on the virulence traits of Candida spp., and its influence is dependent on the exposure time. Non-albicans Candida species presented particular responses to IL-6.

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“…In-depth attempts at elucidating the molecular action of silver NPs were described in the study of Mare, A.D. et al The same synthesis principle was maintained by using the beech bark extracted from Fagus sylvatica L. Nanoparticles synthesis was performed following two pathways that consisted of using two solutions of 1mM, namely AgNO 3 and CH 3 -COOAg solutions. It was revealed that the nanoparticulate products prepared with the acetate solution caused the downregulation of SAP2 expression, which is known to be involved in host cell invasion [ 174 ]. To further complete the mechanistic map of silver NPs, Jia D. and Sun W. focused their attention on the synergistical effect of the nanoparticles and fluconazole to suppress C. albicans activity.…”

Section: Biomaterials and Nanotechnological Approachesmentioning

confidence: 99%

Molecular Mapping of Antifungal Mechanisms Accessing Biomaterials and New Agents to Target Oral Candidiasis

Anuța

Talianu

Dinu-Pîrvu

et al. 2022

IJMS

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Oral candidiasis has a high rate of development, especially in immunocompromised patients. Immunosuppressive and cytotoxic therapies in hospitalized HIV and cancer patients are known to induce the poor management of adverse reactions, where local and systemic candidiasis become highly resistant to conventional antifungal therapy. The development of oral candidiasis is triggered by several mechanisms that determine oral epithelium imbalances, resulting in poor local defense and a delayed immune system response. As a result, pathogenic fungi colonies disseminate and form resistant biofilms, promoting serious challenges in initiating a proper therapeutic protocol. Hence, this study of the literature aimed to discuss possibilities and new trends through antifungal therapy for buccal drug administration. A large number of studies explored the antifungal activity of new agents or synergic components that may enhance the effect of classic drugs. It was of significant interest to find connections between smart biomaterials and their activity, to find molecular responses and mechanisms that can conquer the multidrug resistance of fungi strains, and to transpose them into a molecular map. Overall, attention is focused on the nanocolloids domain, nanoparticles, nanocomposite synthesis, and the design of polymeric platforms to satisfy sustained antifungal activity and high biocompatibility with the oral mucosa.

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In Vitro Antifungal Activity of Silver Nanoparticles Biosynthesized with Beech Bark Extract

Cited by 27 publications

References 61 publications

Therapeutic Effect of Green Synthesized Silver Nanoparticles Using Erodium glaucophyllum Extract against Oral Candidiasis: In Vitro and In Vivo Study

Therapeutic Effect of Green Synthesized Silver Nanoparticles Using Erodium glaucophyllum Extract against Oral Candidiasis: In Vitro and In Vivo Study

Mediation of Candida species growth and virulence by the proinflammatory cytokine IL-6

Molecular Mapping of Antifungal Mechanisms Accessing Biomaterials and New Agents to Target Oral Candidiasis

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