"In vitro" antifungal activity of ozonized sunflower oil on yeasts from onychomycosis

Guerrer, L.V.; Cunha, K. C.; Nogueira, Mara Corrêa Lelles; Cardoso, C. C.; Soares, Márcia Maria Costa Nunes; Almeida, Margarete Teresa Gottardo de

doi:10.1590/s1517-83822012000400011

Cited by 11 publications

(4 citation statements)

References 9 publications

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“…34,38 So far, the demonstrated antimicrobial activity of ozonized oils has been exploited in several fields of application. 29,[39][40][41][42][43] In this study, we demonstrated the antifungal activity of the ozonized sunflower oil eye drops in liposome against four species of Candida. The antimicrobial activity was demonstrated at concentrations several times lower than those of the undiluted product retrieved in ocular surface after the topical of Ozodrop.…”

Section: Discussionmentioning

confidence: 99%

Antimycotic Activity of Ozonized Oil in Liposome Eye Drops against Candida spp.

Celenza

Iorio

Cracchiolo

et al. 2020

Trans. Vis. Sci. Tech.

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Purpose This study aims to investigate the antifungal activity and mechanism of action of ozonized oil eye drops in liposomes (Ozodrop), commercialized as eye lubricant for the treatment of dry eye syndrome and eye inflammation. The activity was tested against four clinical Candida species: C albicans, C glabrata, C krusei , and C orthopsilosis . Methods The antifungal activity of the eye drop solution was ascertained by microdilution method in accordance with EUCAST obtaining the minimum inhibitory concentration for Ozodrop. The mechanism of action was further investigated in C albicans by measuring cell vitality, intracellular reactive oxygen species production, levels of cellular and mitochondrial (∆Ψ m ) membrane potential, and the extent of membrane lipid peroxidation. Results All Candida isolates were susceptible to Ozodrop with minimum inhibitory concentration values ranging from 0.195% (v/v) for C glabrata to 6.25% (v/v) for C orthopsilosis . After 1 hour of exposure at the minimum inhibitory concentration value about 30% of cells were killed, reaching about 70% at the highest Ozodrop value. After Ozodrop exposure, C albicans showed cell membrane depolarization, increased levels of lipid peroxidation, depolarized ∆Ψ m , and increased reactive oxygen species generation. Conclusions The significant increases in reactive oxygen species production cause the accumulation of reactive oxygen species-associated damages leading to progressive Candida cell dysfunction. Translational Relevance The antifungal activity of Ozodrop was demonstrated at concentrations several times lower than the concentration that can be retrieved in ocular surface after its application. The antifungal activity of the eye drops Ozodrop would represent an interesting off-label indication for a product basically conceived as an eye lubricant.

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Section: Discussionmentioning

confidence: 99%

Antimycotic Activity of Ozonized Oil in Liposome Eye Drops against Candida spp.

Celenza

Iorio

Cracchiolo

et al. 2020

Trans. Vis. Sci. Tech.

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“…Previous studies showed that oleozon is non‐toxic and has been used for skin disease and bacterial infections (Almeida, Beatriz, Micheletti, & Arruda, ; Guerrer et al., ). During this experiment, no phytotoxic effects were observed on cucumber leaves after oleozon application.…”

Section: Discussionmentioning

confidence: 99%

Oleozon: A novel control strategy against powdery mildew in cucumber

Zhu

et al. 2017

Journal of Phytopathology

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Ozonized sunflower oil (oleozon) is an effective agent for controlling powdery mildew in cucumber. In this study, the mechanisms of oleozon in the control of powdery mildew were determined. The development of Podosphaera xanthii on cucumber leaves treated with oleozon (2%) and water was investigated at different times after inoculation. The germinating conidia, hyphae and conidiophores of the pathogen were severely damaged by oleozon. No visible phytotoxic effect was observed on cucumber after the application of oleozon. This compound had highly preventive effects as well as curative effects against powdery mildew based on in vivo potted seedling assays.The control effects of oleozon were further confirmed in a greenhouse trial. These results may provide a basis for further development of a natural fungicide against cucumber powdery mildew. K E Y W O R D Santifungal activity, control effect, cucumber powdery mildew, Oleozon, Podosphaera xanthii

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“…Ozonized sunflower oil has demonstrated in vitro antifungal activity against Candida yeasts and has been shown to be as effective as topical ketoconazole in the treatment of tinea pedis [36][37][38]. In a simple-blinded, randomized, controlled trial of 400 participants with culture-confirmed onychomycosis, investigators compared topical ozonized sunflower oil (OLEOZON ® ) twice daily for three months to ketoconazole cream 2% twice daily for 3 months [12].…”

Section: Ozonized Sunflower Oilmentioning

confidence: 99%

Complementary and Alternative Therapies for Onychomycosis: A Systematic Review of the Clinical Evidence

Nickles¹,

Lio²,

Mervak³

2022

Skin Appendage Disord

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Introduction: Onychomycosis is notoriously difficult to treat. While oral antifungals are the most efficacious treatment for onychomycosis, they are contraindicated in certain patient populations, and patients may desire lower risk and accessible alternatives to systemic agents. In this study, we examine the clinical evidence supporting the use of complementary and alternative therapies in the treatment of onychomycosis. Methods: PubMed, Embase, and Cochrane Library were searched for clinical trials, observational studies, and case reports/case series, examining the efficacy of a complementary or alternative therapy for the treatment of onychomycosis. Results: We identified 17 articles studying a complementary and alternative therapy for onychomycosis, including tea tree oil (n = 5), Ageratina pichinchensis (n = 3), Arthrospira maxima (n = 2), natural coniferous resin lacquer (n = 2), Vicks VapoRub® (n = 2), propolis extract (n = 2), and ozonized sunflower oil (n = 1). Conclusion: Given the rise of antifungal resistance, complementary and alternative therapies should continue to be studied as adjunctive or alternative therapy for onychomycosis. While preliminary evidence exists for several complementary and alternative therapies in the treatment of onychomycosis, large-scale, randomized, placebo-controlled trials are needed prior to endorsing their use to patients.

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"In vitro" antifungal activity of ozonized sunflower oil on yeasts from onychomycosis

Cited by 11 publications

References 9 publications

Antimycotic Activity of Ozonized Oil in Liposome Eye Drops against Candida spp.

Antimycotic Activity of Ozonized Oil in Liposome Eye Drops against Candida spp.

Oleozon: A novel control strategy against powdery mildew in cucumber

Complementary and Alternative Therapies for Onychomycosis: A Systematic Review of the Clinical Evidence

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