In vitro anticariogenic and antibiofilm activities of toothpastes formulated with essential oils

Carvalho, Isabela de Oliveira; Purgato, Gislaine Aparecida; Píccolo, Mayra Soares; Pizziolo, Virgínia Ramos; Coelho, Rafael Ribeiro; Díaz, Gaspar; Diaz, Marisa Alves Nogueira

doi:10.1016/j.archoralbio.2020.104834

Cited by 30 publications

(30 citation statements)

References 42 publications

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“…A canine mouthwash was developed as a microemulsion formulation using chlorhexidine (CLX), thymus essential oil (TEO), and oregano essential oil (OEO) as active pharmaceutical ingredients. CLX is one of the most used chemical agents recommended for periodontal disease prevention, and it has been used as an antiseptic in mouthwashes, toothpaste, and oral hygiene products (9,10,11). This agent reduces biofilm formation by altering bacteria's adhesion to teeth and gingival tissues and disrupts the bacterial cell membrane increasing cell permeability (4).…”

Section: Discussionmentioning

confidence: 99%

Formulation and antimicrobial activity evaluation of a 0.2% chlorhexidine canine mouthwash with essential oils

Redondo

Corrales

Rojas

et al. 2021

Vitae

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Background: Periodontal disease is the infection and inflammation of the gums, bones, and tissues involved in teeth support, and it is one of the most common diseases affecting pet dogs. Essential oils have shown antimicrobial activity against bacteria causing periodontal disease; therefore, they are considered potential therapeutic agents. Objectives: The main objective was to formulate and evaluate the antimicrobial activity of a 0.2% chlorhexidine canine mouthwash with essential oils. Methods: Three microemulsion formulations were obtained by constructing a pseudo-ternary phase diagram using the phase titration method. Different surfactant agents were evaluated, and hydrogenated castor oil was selected as the emulsifier agent. The antimicrobial activity of oregano essential oil (Origanum vulgare), thyme essential oil (Thymus vulgaris), and the three formulations were evaluated against Staphylococcus aureus, Streptococcus mutans, Streptococcus salivarius, and Escherichia coli. Results: Pure thyme and oregano essential oils showed higher antimicrobial activity than a 0,2% chlorhexidine solution. The formulations with essential oils plus chlorhexidine and chlorhexidine alone showed antimicrobial activity. The formulation containing only essential oils did not show antimicrobial activity. Conclusions: A canine mouthwash was formulated with chlorhexidine and thyme and oregano essential oil. Based on the evaluation of antimicrobial activity, two of the proposed formulations could be a therapeutic option to reduce the risk and prevent periodontal disease in canines.

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Section: Discussionmentioning

confidence: 99%

Formulation and antimicrobial activity evaluation of a 0.2% chlorhexidine canine mouthwash with essential oils

Redondo

Corrales

Rojas

et al. 2021

Vitae

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“…In addition to the abrasive agent, the disinfectant plays an important role in oral hygiene [17]. For this purpose, the literature has reported the possibility of using herbal products, since they have resulted in enhancing the antimicrobial, anti-biofilm, anti-inflammatory, and antioxidant action of these products [22][23][24][25][26][27]. Among the natural components used for dental application, antimicrobial activity has been demonstrated by the oils of Bowdichia virgilioides Kunth, Copaifera officinalis, Curcuma longa, Eucalyptus citriodora, Melaleuca alternifolia, Pinus strobus, and Ricinus communis, and these could be used as active principles in cleaning products [28][29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, the incorporation of plant extracts into dentifrices has allowed the development of new formulations for oral hygiene, which are effective alternatives to the conventional types available. In vitro studies have evaluated dentifrices for natural teeth based on oils [22] and herbs [23] and have found antimicrobial efficacy against oral microorganisms associated with caries and periodontal disease. Clinical studies have reported that herbal dentifrices [26,27], as well as those containing melaleuca [27], and thymol, eugenol, and eucalyptus [35], oil-based dentifrices reduced the indexes of dental biofilm and gingivitis.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Oil-Based Denture Dentifrices-Organoleptic Characteristics, Physicochemical Properties and Antimicrobial Action

et al. 2021

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Denture dentifrices must be effective and not deleterious to prosthetic devices. This study formulated and evaluated dentifrices based on oils of Copaifera officinalis, Eucalyptus citriodora, Melaleuca alternifolia, Pinus strobus, and Ricinus communis. Organoleptic characteristics (appearance, color, odor, taste), physicochemical properties (pH, density, consistency, rheological, abrasiveness, weight loss, and surface roughness) and antimicrobial (Hole-Plate Diffusion–HPD)/anti-biofilm (Colony Forming Units–CFU) action against Staphylococcus aureus, Streptococcus mutans, and Candida albicans were evaluated. Formulations were compared with water (negative control) and a commercial dentifrice (positive control). The data were analyzed by Kruskal-Wallis and Dunn tests (α = 0.05). The organoleptic and physicochemical properties were adequate. All dentifrices promoted weight losses, with high values for C. officinalis and R. communis, and an increase in surface roughness, without differing from each other. For antimicrobial action, C. officinalis and E. citriodora dentifrices were similar to positive control showing effectiveness against S. mutans and C. albicans and no dentifrice was effective against S. aureus; regarding the anti-biofilm action, the dentifrices were not effective, showing higher CFU counts than positive control for all microorganisms. The dentifrices presented satisfactory properties; and, although they showed antimicrobial action when evaluated by HPD, they showed no effective anti-biofilm action on multispecies biofilm.

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“…Toothpastes containing essential oils of these plants have also been found to be capable of completely destroying S. mutans biofilms. 8 …”

Section: Introductionmentioning

confidence: 99%

Formulation of an Origanum vulgare based dental gel with antimicrobial activity

Badekova

Атажанова

Kačergius

et al. 2021

Journal of Taibah University Medical Sciences

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Objectives This study aims to formulate a new Origanum vulgare anti-caries dental gel with high antimicrobial activity. Methods O. vulgare essential oil was extracted using hydro-distillation, ethanol extraction, gas chromatography/mass spectrometry, and high-performance liquid chromatography methods. Antimicrobial activity of the produced gels with oregano oil and extract was determined through the disco-diffusion method. The effectiveness of O. vulgare essential oil was tested in vitro for Streptococcus mutans biofilm using colorimetric analysis. Results O. vulgare essential oil inhibited the growth of S. mutans biofilm by 98% compared with unexposed control bacteria ( p <0.05 ). Five samples of anti-caries gel (ACDG1, ACDG2, ACDG3, ACDG4, ACDG5) were formulated using the obtained oregano essential oil and extract. Based on the microbiological study results, the ACDG1 and ACDG3 gel samples exhibited high antimicrobial activity against the gram-positive bacterial strains of Staphylococcus aureus and Bacillus subtilis and the yeast fungus Candida albicans and moderate antimicrobial activity against the gram-negative strains of Escherichia coli and Pseudomonas aeruginosa . Conclusion Based on the results of this study, the ACDG3 sample may be considered an anti-caries gel owing to its high antimicrobial activity. This sample has good organoleptic properties compared to other samples, produces relatively high antimicrobial activity, and guards against cariogenic biofilms of S. mutans.

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In vitro anticariogenic and antibiofilm activities of toothpastes formulated with essential oils

Cited by 30 publications

References 42 publications

Formulation and antimicrobial activity evaluation of a 0.2% chlorhexidine canine mouthwash with essential oils

Formulation and antimicrobial activity evaluation of a 0.2% chlorhexidine canine mouthwash with essential oils

Effectiveness of Oil-Based Denture Dentifrices-Organoleptic Characteristics, Physicochemical Properties and Antimicrobial Action

Formulation of an Origanum vulgare based dental gel with antimicrobial activity

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