In Vitro Antibiotic Susceptibilities of Burkholderia mallei (Causative Agent of Glanders) Determined by Broth Microdilution and E-Test

Abstract

In vitro susceptibilities to 28 antibiotics were determined for 11 strains of Burkholderia mallei by the broth microdilution method. The B. mallei strains demonstrated susceptibility to aminoglycosides, macrolides, quinolones, doxycycline, piperacillin, ceftazidime, and imipenem. For comparison and evaluation, 17 antibiotic susceptibilities were also determined by the E-test. E-test values were always lower than the broth dilution values. Establishing and comparing antibiotic susceptibilities of specific B. mallei strains will provide reference information for assessing new antibiotic agents.

Biodefense-Driven Murine Model of Pneumonic Melioidosis

“…Wild-type B. mallei is sensitive to both aminoglycoside and macrolide antibiotics (9), and its genome lacks the amrRAB oprA-encoded multidrug efflux pump (The Institute for Genomic Research). We wondered if the difference in virulence exhibited by B. pseudomallei relative to that of B. mallei (Table 2) could be explained by the loss of the multidrug efflux pump.…”

Evaluation of PCR, DNA hybridization and immunomagnetic separation — PCR for detection of Burkholderia mallei in artificially inoculated environmental samples

“…The human infection can be severe and lifethreatening and always fatal if untreated or misdiagnosed [3]. No vaccines are available against glanders and little is known about the appropriate antibiotic regimen [9]. As a consequence and also because of the lethal and contagious nature of the disease, B. mallei is considered an ideal agent for biological warfare [10].…”

Efficacy of Postexposure Therapy against Glanders in Mice

“…In vitro susceptibility testing has shown that B. mallei is sensitive to a variety of antibiotics, including sulfonamides, aminoglycosides, ciprofloxacin, novobiocin, several tetracyclines, imipenem, and ceftazidime (8)(9)(10). Limited information exists regarding effective antibiotic treatments.…”