In vitro antibiofilm efficacy of Piper betle against quorum sensing mediated biofilm formation of luminescent Vibrio harveyi

Ramanathan, S.; Santhakumari, Sivasubramanian; Ravi, Arumugam Veera

doi:10.1016/j.micpath.2017.07.001

Cited by 45 publications

(12 citation statements)

References 45 publications

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“…To evaluate the antibiofilm potential of phytol, the light and confocal laser scanning microscopic (CLSM) analyses were done by following the method of Srinivasan et al ( 2017a ). After the growth of S. marcescens biofilm with and without of phytol on 1 × 1 cm glass slides, the planktonic cells were removed by washing with distilled water.…”

Section: Methodsmentioning

confidence: 99%

Exploring the Anti-quorum Sensing and Antibiofilm Efficacy of Phytol against Serratia marcescens Associated Acute Pyelonephritis Infection in Wistar Rats

Ramanathan

Ramar

Kannappan

et al. 2017

Front. Cell. Infect. Microbiol.

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Quorum Sensing (QS) mechanism, a bacterial density-dependent gene expression system, governs the Serratia marcescens pathogenesis through the production of virulence factors and biofilm formation. The present study demonstrates the anti-quorum sensing (anti-QS), antibiofilm potential and in vivo protective effect of phytol, a diterpene alcohol broadly utilized as food additive and in therapeutics fields. In vitro treatment of phytol (5 and 10 μg/ml) showed decreasing level of biofilm formation, lipase and hemolysin production in S. marcescens compared to their respective controls. More, microscopic analyses confirmed the antibiofilm potential of phytol. The biofilm related phenomenons such as swarming motility and exopolysccharide productions were also inhibited by phytol. Furthermore, the real-time analysis elucidated the molecular mechanism of phytol which showed downregulation of fimA, fimC, flhC, flhD, bsmB, pigP, and shlA gene expressions. On the other hand, the in vivo rescue effect of phytol was assessed against S. marcescens associated acute pyelonephritis in Wistar rat. Compared to the infected and vehicle controls, the phytol treated groups (100 and 200 mg/kg) showed decreased level of bacterial counts in kidney, bladder tissues and urine samples on the 5th post infection day. As well, the phytol treatment showed reduced level of virulence enzymes such as lipase and protease productions compared to the infected and vehicle controls. Further, the infected and vehicle controls showed increasing level of inflammatory markers such as malondialdehyde (MDA), nitric oxide (NO) and myeloperoxidase (MPO) productions. In contrast, the phytol treatment showed decreasing level of inflammatory markers. In histopathology, the uninfected animal showed normal kidney and bladder structure, wherein, the infected animals showed extensive infiltration of neutrophils in kidney and bladder tissues. In contrast, the phytol treatment showed normal kidney and bladder tissues. Additionally, the toxic effect of phytol (200 mg/kg) was assessed by single dose toxicity analysis. No changes were observed in hematological, biochemical profiles and histopathological analysis of vital organs in phytol treated animals compared to the untreated controls. Hence, this study suggested the potential use of phytol for its anti-QS, antibiofilm and anti-inflammatory properties against S. marcescens infections and their associated inflammation reactions.

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Section: Methodsmentioning

confidence: 99%

Exploring the Anti-quorum Sensing and Antibiofilm Efficacy of Phytol against Serratia marcescens Associated Acute Pyelonephritis Infection in Wistar Rats

Ramanathan

Ramar

Kannappan

et al. 2017

Front. Cell. Infect. Microbiol.

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“…Despite the aforementioned examples, there are many microorganisms for which the presence of the luxS gene and the production of AI-2 is crucial for the formation of biofilms (McNab et al, 2003; Jesudhasan et al, 2010; Jang et al, 2013; Li et al, 2015, 2017; Laganenka et al, 2016; Jani et al, 2017; Laganenka and Sourjik, 2018). The aim of QQ therapy, in this case, would be to maintain the microorganisms in a planktonic form, more sensitive to antibiotics or immune cells’ attack (Chow et al, 2014; Christiaen et al, 2014a; Ryu et al, 2016; Luo et al, 2017; Srinivasan et al, 2017; Mayer et al, 2018; Yu et al, 2018). In pioneer in vivo studies by Fleming and Rumbaugh (2018), for the first time, the effect of massive dispersion of bacteria using the mouse wound infection model was determined.…”

Section: The Second Objection – the Reduction Of Virulence By Quorum mentioning

confidence: 99%

Challenges and Limitations of Anti-quorum Sensing Therapies

Krzyżek

2019

Front. Microbiol.

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Quorum sensing (QS) is a mechanism allowing microorganisms to sense population density and synchronously control genes expression. It has been shown that QS supervises the activity of many processes important for microbial pathogenicity, e.g., sporulation, biofilm formation, and secretion of enzymes or membrane vesicles. This contributed to the concept of anti-QS therapy [also called quorum quenching (QQ)] and the opportunity of its application in fighting against various types of pathogens. In recent years, many published articles reported promising results indicating the possibility of reducing pathogenicity of tested microorganisms and their easier eradication when co-treated with antibiotics. The aim of the present article is to point to the opposite, negative side of the QQ therapy, with particular emphasis on three fundamental properties attributed to anti-QS substances: the selectivity, virulence reduction, and lack of resistance against QQ. This point of view may highlight new directions of research, which should be taken into account in the future before the widespread introduction of QQ therapies in the treatment of people.

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“…The confocal laser scanning microscopic (CLSM) analysis of A. baumannii biofilm was performed as described by Srinivasan et al (2017a). In CLSM analysis, the A. baumannii strains were allowed to form biofilm on the cover glasses in the absence and presence of PCCs, as described in light microscopic analysis.…”

Section: Microscopic Analyses Of Acinetobacter Baumannii Biofilmmentioning

confidence: 99%

Biofilm inhibitory efficiency of phytol in combination with cefotaxime against nosocomial pathogen Acinetobacter baumannii

et al. 2018

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In vitro antibiofilm efficacy of Piper betle against quorum sensing mediated biofilm formation of luminescent Vibrio harveyi

Cited by 45 publications

References 45 publications

Exploring the Anti-quorum Sensing and Antibiofilm Efficacy of Phytol against Serratia marcescens Associated Acute Pyelonephritis Infection in Wistar Rats

Exploring the Anti-quorum Sensing and Antibiofilm Efficacy of Phytol against Serratia marcescens Associated Acute Pyelonephritis Infection in Wistar Rats

Challenges and Limitations of Anti-quorum Sensing Therapies

Biofilm inhibitory efficiency of phytol in combination with cefotaxime against nosocomial pathogen Acinetobacter baumannii

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