In Vitro Antibacterial, Anticancer and Antidiabetic Potential of Freeze-dried Aqueous Borassus flabellifer L. Seed Powder Extract

Abstract

Banu et al.: Therapeutic analysis of Borassus flabellifer L. Seed PowderThe Borassus flabellifer fruit which is one of the summer fruits of India can be considered a promising therapeutic food as it is a good source of bioactive compounds. The aim of this study was to evaluate the antibacterial, anticancer and antidiabetic activity of the locally available aqueous freeze-dried seed powder extract of Borassus flabellifer. Antibacterial activity was evaluated using agar well diffusion assay against gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae). Anticancer activity was studied using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay against HeLa and Vero cells. Antidiabetic activity of the sample was evaluated using alpha amylase and alpha glucosidase inhibition assays. Results revealed that the sample displayed maximum antibacterial activity against gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae); showed satisfactory anticancer activity against the HeLa cells and exhibited potent alpha-amylase and alpha-glucosidase inhibitory activity, thus indicating the use of the Borassus flabellifer seed powder as a potential therapeutic agent for the development of drugs and functional foods.

Effect of Extraction Methods on the Antioxidants and Alpha-Glucosidase Inhibitory Activity of Borassus flabellifer L. Fruit Fiber

“…An increase in absorbance correlates with the activity of the sample. 26,36,37 From this study, YLFF showed negative alpha-glucosidase inhibitory activity at sample concentrations ranging from 100 -2000 µg/mL. Meanwhile, according to data presented in Table 2, the OLFF sample showed very low alpha-glucosidase inhibitory activity with % inhibition on minimum test concentration of 100 µg/mL ranging from 0.3 -2.4 % and on maximum test concentration of 2000 µg/mL ranging from 8.6 -23%.…”

Metabolite profiling from different parts of tender fruit endosperm of Borassus flabellifer L. (ice apple)

“…abellifer seed powder as a prospective cure for a deadly illness. 9 When galactomannan was originally discovered in the so palm kernel, it was discovered to contain mannose (78.32%) and galactose (27.64-28.06%). Current research on the in vitro free radical scavenging potential of the carotenoids in palmyra fruit pulp revealed the types of free sugars and polysaccharides that are prevalent in the kernel.…”

“…The extract of B. flabellifer freeze-dried seed powder demonstrated maximal inhibition of Gram-negative bacteria ( E. coli and Klebsiella pneumoniae ), revealed acceptable anti-cancer, and demonstrated significant α-amylase and α-glucosidase inhibitory activity, confirming the utilization of B. flabellifer seed powder as a prospective cure for a deadly illness. 9 When galactomannan was originally discovered in the soft palm kernel, it was discovered to contain mannose (78.32%) and galactose (27.64–28.06%). Current research on the in vitro free radical scavenging potential of the carotenoids in palmyra fruit pulp revealed the types of free sugars and polysaccharides that are prevalent in the kernel.…”