In vitro antibacterial activity of endodontic sealers

Pizzo, Giuseppe; Giammanco, Anna; Cumbo, Enzo Maria Giuseppe; Nicolosi, Giuseppe; Gallina, Giuseppe

doi:10.1016/j.jdent.2005.03.001

Cited by 96 publications

(96 citation statements)

References 34 publications

(38 reference statements)

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“…Regarding the time elapsed until the occurrence of E. faecalis leakage, broth turbidity was not observed in any of the groups up to the 10th day of the experiment. According to Pizzo et al, (13), AH Plus sealer, used in this study, presents bacterial inhibition within the first 24 hours, which may have delayed bacterial penetration but was not able to completely prevent coronal leakage of E. faecalis. Therefore, an important role of the endodontic obturation is the formation of a physical barrier to prevent bacteria from reaching the apical region and periapical tissues (16).…”

Section: Discussionmentioning

confidence: 88%

Influence of the length of remaining root canal filling and post space preparation on the coronal leakage of Enterococcus faecalis

Mozini¹,

Vansan²,

Sousa‐Neto³

et al. 2009

Braz. J. Microbiol.

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This study evaluated the sealing ability of different lengths of remaining root canal filling and post space preparation against coronal leakage of Enterococcus faecalis. Forty-one roots of maxillary incisors were biomechanically prepared, maintaining standardized canal diameter at the middle and coronal thirds. The roots were autoclaved and all subsequent steps were undertaken in a laminar flow chamber. The canals of 33 roots were obturated with AH Plus sealer and gutta-percha. The root canal fillings were reduced to 3 predetermined lengths (n=11): G1=6 mm, G2=4 mm and G3=2 mm. The remaining roots served as positive and negative controls. Bacterial leakage test apparatuses were fabricated with the roots attached to Eppendorf tubes keeping 2 mm of apex submerged in BHI in glass flasks. The specimens received an E. faecalis inoculum of 1 x 107 cfu/mL every 3 days and were observed for bacterial leakage daily during 60 days. Data were submitted to ANOVA, Tukey's test and Fisher's test. At 60 days, G1 (6 mm) and G2 (4 mm) presented statistically similar results (p>0.05) (54.4% of specimens with bacterial leakage) and both groups differed significantly (p<0.01) from G3 (2 mm), which presented 100% of specimens with E. faecalis leakage. It may be concluded that the shortest endodontic obturation remnant leaked considerably more than the other lengths, although none of the tested conditions avoids coronal leakage of E. faecalis.

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Section: Discussionmentioning

confidence: 88%

Influence of the length of remaining root canal filling and post space preparation on the coronal leakage of Enterococcus faecalis

Mozini¹,

Vansan²,

Sousa‐Neto³

et al. 2009

Braz. J. Microbiol.

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“…1 The application of endodontic sealers after biomechanical cleaning, which is a common procedure, has been used to prevent and cure periapical periodontitis. 2 However, it has been reported that even after rigid chemomechanical preparation, microorganisms in the lateral canals, dentinal tubules, and apical ramifications still cannot be cleaned thoroughly. 3,4 If the access cavity is sealed incompletely, passages for bacteria, fluid, and chemical substance penetration are apt to be created when the root canals are not well obturated.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical and biological properties of a novel injectable polyurethane system for root canal filling

Wang¹,

Zuo²,

Zhao³

et al. 2015

IJN

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A root canal sealer with antibacterial activity can be efficacious in preventing reinfection that results from residual microorganisms and/or the leakage of microorganisms. In the present study, a series of injectable, self-curing polyurethane (PU)-based antibacterial sealers with different concentrations of silver phosphate (Ag 3 PO 4 ) were fabricated. Subsequently, their physicochemical properties, antibacterial abilities, and preliminary cytocompatibilities were evaluated. The results indicated that the fabricated PU-based sealers can achieve a high conversion rate in a short amount of time. More than 95% of the isocyanate group of PU sealers with 3 wt% (PU3) and 5 wt% (PU5) concentrations of Ag 3 PO 4 were included in the curing reaction after 7 hours. With the exception of those for film thickness for PU5, the results of setting time, film thickness, and solubility were able to meet the requirements of the International Organization for Standardization. The antibacterial tests showed that PU3 and PU5 exhibit stronger antimicrobial effects than that achieved with 1 wt% Ag 3 PO 4 (PU1) and AH Plus (positive control) against Streptococcus mutans . The cytocompatibility evaluation revealed that the PU1 and PU3 sealers possess good cytocompatibility and low cytotoxicity. These results demonstrate that the PU3 sealer offers good physicochemical and antimicrobial properties along with cytocompatibility, which may hold great application potential in the field of root canal fillings.

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“…This difference may be explained by the fact that the sealers used in the present study were not fresh when put into contact with the biofilm, whereas the antimicrobial activity of test sealers depends on the time interval between mixing and testing. 29 In the present study, the sealers were put into contact with biofilm just 56 h after manipulation, whereas Wang et al 23 and Poggio et al 17 used the sealer freshly manipulated. Studies report that the antimicrobial action of AH Plus is attributed to formaldehyde release over a short period of time during the setting time.…”

Section: Discussionmentioning

confidence: 86%

“…1,29 Poggio et al 17 used ADT with freshly mixed endodontic sealer (Endomethasone C, Argoseal, Bioseal Normal, Acroseal, AH Plus, Sicura Seal) after 24 and 48 h, and found low antibacterial activity for AH Plus in the periods tested. Wang et al 23 evaluated the proportions of dead and live bacteria inside the dentinal tubules after exposure to root canal sealers by confocal laser scanning microscopy, and reported antimicrobial activity for AH Plus after 1 and 7 days, unlike the results of the present study, which found no antimicrobial activity after 7 days.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

et al. 2016

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Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm Abstract: Enterococcus faecalis are gram positive bacteria that can mostly resist endodontic therapy, inducing persistent infection in the root canal system. Endodontic sealers with antimicrobial activity may help eliminate residual microorganisms that survive endodontic treatment. The present study aimed at comparing the antimicrobial activity of Acroseal, Sealapex and AH Plus endodontic sealers in an in vitro biofilm model. Bovine dentin specimens (144) were prepared, and twelve blocks for each sealer and each experimental time point (2, 7 and 14 days) were placed and left in contact with plates containing inoculum of E. faecalis (ATCC 51299), to induce biofilm formation. After 14 days, the samples were transferred to another plate with test sealers and kept at 37°C and 5% CO 2 for 2, 7 and 14 days. The specimens without sealers were used as a control for each period. The samples were agitated in a sonicator after each experiment. The suspensions were agitated in a vortex mixer, serially diluted in saline, and triple plated onto m-Enterococcus agar. Colonyforming units were counted, and the data were statistically analyzed using ANOVA, Shapiro-Wilk and Kruskal-Wallis one-way tests (p < 0.05) to determine antimicrobial potential. Sealapex showed significant differences at all the experimental time points, in comparison with all the other groups. AH Plus and Acroseal showed antimicrobial activity only on the 14 th experimental day. Neither of the sealers tested were able to completely eliminate the biofilm. Sealapex showed the highest antimicrobial activity in all the experimental periods. The antimicrobial activity of all the sealers analyzed increased over time.

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In vitro antibacterial activity of endodontic sealers

Cited by 96 publications

References 34 publications

Influence of the length of remaining root canal filling and post space preparation on the coronal leakage of Enterococcus faecalis

Influence of the length of remaining root canal filling and post space preparation on the coronal leakage of Enterococcus faecalis

Physicochemical and biological properties of a novel injectable polyurethane system for root canal filling

Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

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