In vitro antibacterial activity of ZnO and Nd doped ZnO nanoparticles against ESBL producing Escherichia coli and Klebsiella pneumoniae

Hameed, A.S. Haja; Karthikeyan, Chandrasekaran; Ahamed, A. Jafar; Thajuddin, Nooruddin; Alharbi, Naiyf S.; Alharbi, Sulaiman Ali; Ravi, G.

doi:10.1038/srep24312

Cited by 303 publications

(108 citation statements)

References 53 publications

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“…The damage produced by ZnO@ZIF‐8 A was comparable to that generated by free ampicillin, suggesting its potent antimicrobial activity. The observed morphological changes of the bacterial cell are in accordance with the previously reported studies . The potent antimicrobial activity of the ZnO@ZIF‐8 particles could be attributed to the released zinc ions and oxidative damage due to ZnO, combined with increased membrane permeability and subsequent antibiotic uptake by the bacterial cells, leading to cell death.…”

Section: Resultssupporting

confidence: 90%

Synergistic Antimicrobial Activity in Ampicillin Loaded Core‐Shell ZnO@ZIF‐8 Particles

et al. 2019

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Combination therapy with the use of nanomaterials and antibiotics is an effective approach to combat increasing antimicrobial resistance. Herein, the synthesis and antimicrobial activity of core‐shell Zinc oxide ‐ Zeolitic Imidazolate Framework‐8 (ZnO@ZIF‐8) particles loaded with ampicillin is reported. The efficacy of the above system has been evaluated against Gram‐negative Escherichia coli (E. coli) and Gram‐positive Staphylococcus aureus (S. aureus) strains. The entrapment of ampicillin within the ZIF‐8 shell of the particles allowed its controlled and pH‐responsive release under in vitro conditions. Ampicillin loaded ZnO@ZIF‐8 particles have exhibited enhanced antimicrobial activity as reflected by the minimum inhibitory concentration (MIC) values of 12.50 and 48 μg mL−1 against E. coli and S. aureus, respectively. This was also evident by a several‐fold reduction observed in the bacterial population during the time‐kill assays. The values of fractional inhibitory concentration indices (FICI), assessed for different components, have revealed the ZnO@ZIF‐8/ampicillin system inflicts a synergistic activity against E. coli and an additive activity against S. aureus. Cell membrane disruption is attributed to cell death. Finally, cytotoxicity study against mammalian cell lines has indicated the safety profiles of the particles.

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Section: Resultssupporting

confidence: 90%

Synergistic Antimicrobial Activity in Ampicillin Loaded Core‐Shell ZnO@ZIF‐8 Particles

et al. 2019

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“…The produced ROS may create some electron holes on the surface of bacterial cells, which facilitate the entry of ZnO NP and Ams both. The free radicals generated by ZnO NP may oxidize the ESBL enzyme of K. pneumoniae, which is responsible for the resistivity towards Ams [55,56]. ZnO NP hampers the ESBL enzyme present in the cytoplasm and also changes the permeability of the cell wall of bacteria.…”

Section: Discussionmentioning

confidence: 99%

Preparation and Evaluation of the ZnO NP–Ampicillin/Sulbactam Nanoantibiotic: Optimization of Formulation Variables Using RSM Coupled GA Method and Antibacterial Activities

et al. 2019

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Nanoparticles (NPs) possessing antibacterial activity represent an effective way of overcoming bacterial resistance. In the present work, we report a novel formulation of a nanoantibiotic formed using Ampicillin/sulbactam (Ams) and a zinc oxide nanoparticle (ZnO NP). 'ZnO NP-Ams' nanoantibiotic formulation is optimized using response surface methodology coupled genetic algorithm approach. The optimized formulation of nanoantibiotic (ZnO NP: 49.9 µg/mL; Ams: 33.6 µg/mL; incubation time: 27 h) demonstrated 15% enhanced activity compared to the unoptimized formulation against K. pneumoniae. The reactive oxygen species (ROS) generation was directly proportional to the interaction time of nanoantibiotic and K. pneumoniae after the initial lag phase of 18 h as evident from 2s'-7'-Dichlorodihydrofluorescein diacetate assay. A low minimum inhibitory concentration (6.25 µg/mL) of nanoantibiotic formulation reveals that even a low concentration of nanoantibiotic can prove to be effective against K. pneumoniae. The importance of nanoantibiotic formulation is also evident by the fact that the 100 µg/mL of Ams and 25 µg of ZnO NP was required individually to inhibit the growth of K. pneumonia, whereas only 6.25 µg/mL of optimized nanoantibiotic formulation (ZnO NP and Ams in the ratio of 49.9: 33.6 in µg/mL and conjugation time of 27 h) was needed for the same.

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“…ZnO NPs have shown antimicrobial activity against bacteria and even antibacterial activity against spores [4][5][6][7]. Amongst all the inorganic semiconducting nanoparticles, ZnO NPs have attracted the scientific community on the grounds that these nanoparticles can effectively be synthesized and are believed to be biocompatible, biodegradable, and nontoxic for clinical applications [8]. It is taken as an alternating material for TiO 2 due to its properties, for example strength and its tendency to be easily synthesized in various shapes and sizes.…”

Section: Introductionmentioning

confidence: 99%

Green route synthesis of ZnO nanoparticles mediated by Melia azedarach for microbiological applications

et al. 2020

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Green synthesis technique of nanoparticles has advantages over physical and chemical methods due to its simplicity in preparation, environment friendly nature and less time consuming. We report a green approach for the synthesis of zincoxide nanoparticles using leave extract from melia azedarach. Melia azedarach plant has been traditionally famous for its antibacterial properties. The plant extract acts as reducing and capping agent, while zinc nitrate hexahydrate [Zn(NO 3 ) 2 .6H 2 O] was used as a precursor. The formation of ZnO nanoparticles was observed by the color changes during the reaction. Structural, optical and morphological properties of ZnO nanoparticles were determined using x-ray diffraction, scanning electron microscope, Fourier transform spectroscopy and UV-vis spectroscopy. XRD analysis confirms the formation of wurtzite hexagonal structure NPs with significantly small average crystallite size of 7.0 nm. The energy band gap determined by UV-vis spectroscopy was measured between 2.9 eV to 5.4 eV. The antibacterial activity of ZnO nanoparticles was tested against E. coli bacteria and significant results were obtained. The anti-bacterial study proved that green-rout prepared ZnO nanoparticles are the best choice for future research concerning antibacterial activity.

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In vitro antibacterial activity of ZnO and Nd doped ZnO nanoparticles against ESBL producing Escherichia coli and Klebsiella pneumoniae

Cited by 303 publications

References 53 publications

Synergistic Antimicrobial Activity in Ampicillin Loaded Core‐Shell ZnO@ZIF‐8 Particles

Synergistic Antimicrobial Activity in Ampicillin Loaded Core‐Shell ZnO@ZIF‐8 Particles

Preparation and Evaluation of the ZnO NP–Ampicillin/Sulbactam Nanoantibiotic: Optimization of Formulation Variables Using RSM Coupled GA Method and Antibacterial Activities

Green route synthesis of ZnO nanoparticles mediated by Melia azedarach for microbiological applications

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