In vitro Antibacterial Activity of an Active Metabolite Isolated from Streptomyces Species

Sultan, Md. Zakir; Khatune, Naznin Ara; Sathi, Zakia Sultana; ., Md. Shah Alam Bhuiyan; Sadik, Golam; ., M. Akteruzzaman Choudury; Gafur, M. A.; Rahman, M.M. Hafizur

doi:10.3923/biotech.2002.100.106

Cited by 9 publications

(5 citation statements)

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“…The optimal initial pH for the production of secondary metabolites by Streptomyces sp. was found to be neutral pH, which was reported in several studies [ 26 , 27 ]. Mutants selected through experiments were found to produce the optimal active substance when the pH level was between 7 and 8.5.…”

Section: Discussionsupporting

confidence: 70%

Culture optimization of Streptomyces sp. KRA16-334 for increased yield of new herbicide 334-W4

Kim,

Jang,

Choi

2024

PLoS ONE

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This study aimed to isolate actinomycetes that exhibit strong herbicidal activity, identify compounds active against weeds, and researching methods to improve the production of these compounds through culture optimization to establish a foundation for the development of environmentally friendly bioherbicides. 334-W4, one of the herbicidal active substances isolated from the culture broth of Streptomyces sp. KRA16-334, exhibited herbicidal activity against various weeds. The molecular formula of 334-W4 was determined to be C16H26N2O6, based on ESI-MS (m/z) and 1H and 13C NMR spectral data. It had molecular weight 365.1689 [M+Na] and 343.1869 [M+H], indicating the presence of the epoxy-β-aminoketone moiety based on HMBC correlations. Additionally, selective culture was possible depending on the addition of trifluoroacetic acid (TFA) during culture with GSS medium. Experiments confirmed that exposure of the KRA16-334 strain to UV irradiation (254 nm, height 17 cm) for 45 seconds improved the yield of the active substance (334-W4) by over 200%. As a result of examining yields of active materials of four mutants selected through optimization of culture conditions such as temperature, agitation, and initial pH, the yield of one mutant 0723–8 was 264.7 ± 12.82 mg/L, which was 2.8-fold higher than that of wild-type KRA16-334 at 92.8 ± 5.48 mg/L.

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Section: Discussionsupporting

confidence: 70%

Culture optimization of Streptomyces sp. KRA16-334 for increased yield of new herbicide 334-W4

Kim,

Jang,

Choi

2024

PLoS ONE

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“…Previous studies usually started the optimization process by selecting the basal production medium. We found that the complex media, especially yeast extract glucose broth medium and yeast extract-malt extract-dextrose broth medium, were often used as basal production medium ( Sultan et al, 2002 ; Narayana & Vijayalakshmi, 2008 ; Sharon, Daniel & Shenbagarathai, 2014 ). These media had both carbon and nitrogen sources that supported the production of Streptomyces sp.…”

Section: Resultsmentioning

confidence: 99%

“…Generally, it was observed that Streptomyces species showed a progressive increase of biomass during the first 3 to 5 days of incubation. BC production usually started on the 5th to 7th day and maximum activity was often recorded on the 7th to 14th day ( Sultan et al, 2002 ; Oskay, 2011 ; Rakesh et al, 2014 ; Bundale et al, 2015 ). We also found that two phases were observed during the propagation of BC producers.…”

Section: Resultsmentioning

confidence: 99%

“…The pH of the medium greatly influenced the production of BCs of Streptomyces species by affecting the activity of cellular enzymes correlated with the biosynthesis of BCs ( Bundale et al, 2015 ). Several studies reported that the optimal initial medium pH for BC production of the Streptomyces species depended on their optimal growth in a neutral environment ( Sultan et al, 2002 ; Oskay, 2011 ; Reddy, Ramakrishna & Rajagopal, 2011 ). Therefore, we found that most BCs were optimally produced at a pH level close to 7.…”

Section: Resultsmentioning

confidence: 99%

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Characterization of Streptomyces sp. KB1 and its cultural optimization for bioactive compounds production

Lertcanawanichakul

Sahabuddeen

2023

PeerJ

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Background Bioactive compounds (BCs) from natural resources have been extensively studied because of their use as models in the development of novel and important medical and biopreservative agents. One important source of BCs is microorganisms, particularly terrestrial bacteria of the order Actinomycetales. Methods We characterized Streptomyces sp. KB1 by observing its morphology, physiology, and growth on different media using biochemical tests, optimizing cultural conditions by changing one independent variable at a time. Results Streptomyces sp. KB1 (TISTR 2304) is a gram-positive and long filamentous bacteria that forms straight to flexuous (rectiflexibile) chains of globose-shaped and smooth-surfaced spores. It can grow under aerobic condition s only at a temperature range of 25–37 °C and initial pH range of 5–10 in the presence of sodium chloride 4% (w/v). Therefore, it is considered an obligate aerobe, mesophilic, neutralophilic, and moderately halophilic bacteria. The isolate grew well on peptone-yeast extract iron, Luria Bertani (LB), and a half-formula of LB (LB/2), but could not grow on MacConkey agar. It utilized fructose, mannose, glucose, and lactose as its carbon source along with acid production and showed positive reactions to casein hydrolysis, gelatin liquefaction, nitrate reduction, urease, and catalase production. Streptomyces sp. KB1 (TISTR 2304) could produce the maximum number of BCs when 1% of its starter was cultivated in a 1,000 ml baffled flask containing 200 ml of LB/2 broth with its initial pH adjusted to 7 with no supplemental carbon source, nitrogen source, NaCl, or trace element at 30 °C, shaken at 200 rpm in an incubator for 4 days.

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“…As actinobactérias são um grupo de bactérias filamentosas, Gram-positivas, que desenvolvem hifas morfologicamente semelhantes às dos fungos, porém, com diâmetros menores, sendo que, fisiologicamente, se assemelham a bactérias (SULTAN et al, 2002). São capazes de produzir metabólitos secundários de importância econômica, enzimas e compostos antimicrobianos ou antifúngicos, com aplicações potenciais para biotecnologia, biofarmacêutica, uso clínico e biocontrole (LAMILLA et al, 2016).…”

Section: Introductionunclassified

Efeito Da Antropização Sobre a Produção Da Enzima Xilanase Em Actinobatérias

Sousa¹,

Lira²,

Martins³

et al. 2018

Enci Bio

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RESUMO As actinobactérias são um grupo de bactérias filamentosas capazes de produzir metabólitos secundários de importância econômica, entre esses está a xilanase, um complexo extracelular que ocorre em micro-organismos, responsável pela degradação da hemicelulose xilana, com o exposto, este trabalho foi realizado com o objetivo de verificar a atividade xilanolítica de actinobactérias isoladas da cidade de Quixadá-CE, assim como comparar essa atividade entre uma área conservada e outra degradada. As amostras de solo foram coletadas na Fazenda Não Me Deixes, onde foram obtidas 38 cepas, sendo 19 de área antropizada e 19 de área conservada, que foram testadas para a produção de xilanase. Do total de cepas testadas, 26 apresentaram a capacidade de produzir xilanase. As cepas oriundas da área antropizada apresentaram uma maior produção da enzima. O isolado QX 7 obteve um maior índice enzimático e, portanto, potencial na produção de enzima xilanase extracelular que pode ser usada em aplicações industriais. PALAVRAS-CHAVE: Atividade enzimática, caatinga, solo.

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In vitro Antibacterial Activity of an Active Metabolite Isolated from Streptomyces Species

Cited by 9 publications

References 0 publications

Culture optimization of Streptomyces sp. KRA16-334 for increased yield of new herbicide 334-W4

Culture optimization of Streptomyces sp. KRA16-334 for increased yield of new herbicide 334-W4

Characterization of Streptomyces sp. KB1 and its cultural optimization for bioactive compounds production

Efeito Da Antropização Sobre a Produção Da Enzima Xilanase Em Actinobatérias

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