In vitro antibacterial activity of extracts from Samoan medicinal plants and their effect on proliferation and migration of human fibroblasts

Fraňková, Adéla; Vištějnová, Lucie; Merinas-Amo, Tania; Leheckova, Z.; Doskočil, Ivo; Soon, Julian Wong; Kudera, Tomas; Laupua, F.; Alonso-Moraga, Ángeles; Kokoška, Ladislav

doi:10.1016/j.jep.2020.113220

Cited by 16 publications

(14 citation statements)

References 56 publications

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“…One of the nonantibiotic materials with antibacterial activity is a natural product. The antimicrobial activity of natural products has been reported in many papers, and some of these products even show antimicrobial effects against MDR bacteria [10][11][12][13][14][15]. Another candidate for nonantibiotic materials is antibacterial nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Selenium Nanoparticles as Candidates for Antibacterial Substitutes and Supplements against Multidrug-Resistant Bacteria

et al. 2021

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In recent years, multidrug-resistant (MDR) bacteria have increased rapidly, representing a major threat to human health. This problem has created an urgent need to identify alternatives for the treatment of MDR bacteria. The aim of this study was to identify the antibacterial activity of selenium nanoparticles (SeNPs) and selenium nanowires (SeNWs) against MDR bacteria and assess the potential synergistic effects when combined with a conventional antibiotic (linezolid). SeNPs and SeNWs were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), zeta potential, and UV-visible analysis. The antibacterial effects of SeNPs and SeNWs were confirmed by the macro-dilution minimum inhibitory concentration (MIC) test. SeNPs showed MIC values against methicillin-sensitive S. aureus (MSSA), methicillin-resistant S. aureus (MRSA), vancomycin-resistant S. aureus (VRSA), and vancomycin-resistant enterococci (VRE) at concentrations of 20, 80, 320, and >320 μg/mL, respectively. On the other hand, SeNWs showed a MIC value of >320 μg/mL against all tested bacteria. Therefore, MSSA, MRSA, and VRSA were selected for the bacteria to be tested, and SeNPs were selected as the antimicrobial agent for the following experiments. In the time-kill assay, SeNPs at a concentration of 4X MIC (80 and 320 μg/mL) showed bactericidal effects against MSSA and MRSA, respectively. At a concentration of 2X MIC (40 and 160 μg/mL), SeNPs showed bacteriostatic effects against MSSA and bactericidal effects against MRSA, respectively. In the synergy test, SeNPs showed a synergistic effect with linezolid (LZD) through protein degradation against MSSA and MRSA. In conclusion, these results suggest that SeNPs can be candidates for antibacterial substitutes and supplements against MDR bacteria for topical use, such as dressings. However, for use in clinical situations, additional experiments such as toxicity and synergistic mechanism tests of SeNPs are needed.

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Section: Introductionmentioning

confidence: 99%

Selenium Nanoparticles as Candidates for Antibacterial Substitutes and Supplements against Multidrug-Resistant Bacteria

et al. 2021

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“…validate a subset of traditional uses (10)(11)(12)(13), the molecular mechanisms of P. insularum remain enigmatic. Thus far, a class of bioactive compounds common in Psychotria species (cyclotides) have been investigated, but these compounds have not been found in P. insularum (14,15); hence the bioactive compounds mediating the biological activities of P. insularum remain to be identified.…”

Section: Significancementioning

confidence: 99%

“…Despite these diverse uses and wide distribution across the Pacific and in South America, the medicinal use of P. insularum has only been documented in the Samoan islands. Although model organisms have been used to validate a subset of traditional uses ( 10 – 13 ), the molecular mechanisms of P. insularum remain enigmatic. Thus far, a class of bioactive compounds common in Psychotria species (cyclotides) have been investigated, but these compounds have not been found in P. insularum ( 14 , 15 ); hence the bioactive compounds mediating the biological activities of P. insularum remain to be identified.…”

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confidence: 99%

Functional genomics and metabolomics advance the ethnobotany of the Samoan traditional medicine “matalafi”

Molimau-Samasoni

Woolner

Foliga³

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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The leaf homogenate of Psychotria insularum is widely used in Samoan traditional medicine to treat inflammation associated with fever, body aches, swellings, wounds, elephantiasis, incontinence, skin infections, vomiting, respiratory infections, and abdominal distress. However, the bioactive components and underlying mechanisms of action are unknown. We used chemical genomic analyses in the model organism Saccharomyces cerevisiae (baker’s yeast) to identify and characterize an iron homeostasis mechanism of action in the traditional medicine as an unfractionated entity to emulate its traditional use. Bioactivity-guided fractionation of the homogenate identified two flavonol glycosides, rutin and nicotiflorin, each binding iron in an ion-dependent molecular networking metabolomics analysis. Translating results to mammalian immune cells and traditional application, the iron chelator activity of the P. insularum homogenate or rutin decreased proinflammatory and enhanced anti-inflammatory cytokine responses in immune cells. Together, the synergistic power of combining traditional knowledge with chemical genomics, metabolomics, and bioassay-guided fractionation provided molecular insight into a relatively understudied Samoan traditional medicine and developed methodology to advance ethnobotany.

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“…Two clinical drug-resistant isolates of S. aureus (SA 2, and SA 3) and one epidemic MRSA strain (EMRSA-15) were obtained from the Motol University Hospital (Prague, Czech Republic). Based on the previously determined MICs values [ 51 , 52 , 53 ], the clinical isolates were identified to be resistant as follows: SA 2—resistant to oxacillin (MIC 68 µg/mL), gentamicin (MIC 16 µg/mL), and tetracycline (MIC 8 µg/mL); SA 3—resistant to gentamicin (MIC 8 µg/mL), and penicillin (MIC 18.67 µg/mL); EMRSA-15—resistant to oxacillin (MIC 99.56 µg/mL), and penicillin (MIC 16 µg/mL). The clinical isolates were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as described previously [ 51 ].…”

Section: Methodsmentioning

confidence: 99%

In Vitro Antibacterial Effect of the Methanolic Extract of the Korean Soybean Fermented Product Doenjang against Staphylococcus aureus

Laloučková

Malá

Maršík

et al. 2021

Animals

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Ultra-high performance liquid chromatography/mass spectrometry showed soyasaponin I and the isoflavones daidzein, genistein, and glycitein to be the main components of the methanolic extract of the Korean soybean fermented product doenjang, which is known to be a rich source of naturally occurring bioactive substances, at average contents of 515.40, 236.30, 131.23, and 29.00 ng/mg, respectively. The antimicrobial activity of the methanolic extract of doenjang against nine Staphylococcusaureus strains was determined in vitro by the broth microdilution method to investigate its potential to serve as an alternative antibacterial compound. The results suggest that the extract is an effective antistaphylococcal agent at concentrations of 2048–4096 µg/mL. Moreover, the tested extract also showed the ability to inhibit the growth of both methicillin-sensitive and methicillin-resistant animal and clinical S. aureus isolates. The growth kinetics of the chosen strains of S. aureus at the minimum inhibitory concentration of the methanolic extract of doenjang support the idea that the tested extract acts as an antibacterial compound. To the best of our knowledge, this is the first report on the antistaphylococcal action of the methanolic extract of doenjang thus, additional studies including in vivo testing are necessary to confirm this hypothesis.

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In vitro antibacterial activity of extracts from Samoan medicinal plants and their effect on proliferation and migration of human fibroblasts

Cited by 16 publications

References 56 publications

Selenium Nanoparticles as Candidates for Antibacterial Substitutes and Supplements against Multidrug-Resistant Bacteria

Selenium Nanoparticles as Candidates for Antibacterial Substitutes and Supplements against Multidrug-Resistant Bacteria

Functional genomics and metabolomics advance the ethnobotany of the Samoan traditional medicine “matalafi”

In Vitro Antibacterial Effect of the Methanolic Extract of the Korean Soybean Fermented Product Doenjang against Staphylococcus aureus

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