In vitro antibacterial activity and volatile characterisation of organic Apis mellifera ligustica (Spinola, 1906) beeswax ethanol extracts

Felicioli, Antonio; Cilia, Giovanni; Mancini, Simone; Turchi, Barbara; Galaverna, Gianni; Cirlini, Martina; Cerri, Domenico; Fratini, Filippo

doi:10.1016/j.fbio.2019.04.004

Cited by 21 publications

(17 citation statements)

References 60 publications

(48 reference statements)

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“…The biological (antimicrobial, antioxidant) and nutritional properties of BCP and BB are directly related to their composition, which in turn is directly related to their botanical origin [ 53 , 54 ]. This is the case for other bee products such as honey or even beeswax [ 55 , 56 , 57 , 58 ].…”

Section: Introductionmentioning

confidence: 99%

Biological Properties of Bee Bread Collected from Apiaries Located across Greece

et al. 2021

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Bee bread is the only fermented product of the beehive. It constitutes the main source of proteins, lipids, vitamins, and macro- and microelements in honeybee nutrition and it exerts antioxidant and antimicrobial properties, though research on these aspects has been limited so far. In this study 18 samples of Greek bee bread, two of which were monofloral, were collected during different seasons from diverse locations such as Crete and Mount Athos and were tested for their bioactivity. Samples were analyzed for their antibacterial properties, antioxidant activity, total phenolic content (TPC), and total flavonoid content (TFC). The antimicrobial activity of each sample was tested against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Salmonella typhimurium. Our data demonstrate that all samples exert inhibitory and most of them bactericidal activity against at least two pathogens. Furthermore, all samples exert significant antioxidant activity, where the monofloral Castanea Sativa sample demonstrated superior antioxidant activity. Nevertheless, the antioxidant and antimicrobial activity were not strongly correlated. Furthermore, machine learning methods demonstrated that the palynological composition of the samples is a good predictor of their TPC and ABTS activity. This is the first study that focuses on the biological properties of Greek bee bread and demonstrates that bee bread can be considered a functional food and a possible source of novel antimicrobial compounds.

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Section: Introductionmentioning

confidence: 99%

Biological Properties of Bee Bread Collected from Apiaries Located across Greece

et al. 2021

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“…To date, it has been established that several honey components play a crucial role for its antimicrobial properties [ 2 ]. Furthermore, royal jelly, beeswax and bee venom highlighted antimicrobial activity against a wide range of bacteria [ 3 , 4 , 5 , 6 ]. The antimicrobial activity of honey is due to physicochemical properties, such as the high content of reducing sugars, high viscosity, high osmotic pressure, low pH, low water activity (AW), low protein content and the presence of hydrogen peroxide [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Honey Samples from Ukraine

Cilia

Fratini

Marchi

et al. 2020

Veterinary Sciences

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The employment of natural substances such as beehive products with a preventive and therapeutic purpose has been a widespread custom since ancient times. In this investigation, the antibacterial activity of 41 honey samples from different Ukraine regions has been evaluated. For each honey, melissopalynological and physico-chemical analysis were performed in order to determine botanical origin, pH, glucose and fructose contents and free acidity. So, antibacterial activity against Staphylococcusaureus CCM 4223, Listeria monocytogenes ATCC 7644, Salmonella enterica serovar Typhimurium CCM 3807 and Escherichia coli ATCC 25922 was assessed through the determination of MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) values by the microdilutions method. The results show that the most susceptible bacterial strain was L. monocytogenes. Its growth was inhibited at a honey concentration ranging from 0.094 to 0.188 g/mL. The most resistant bacterial strain was S. aureus. As concerns MBC values, L. monocytogenes was the most susceptible bacteria, while S. aureus was the most resistant. Helianthus spp. honeys was the most effective against all tested bacterial strains, followed by Robinia spp. and multifloral honeys. Promising results for MIC tests have been found for Brassica spp.

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“…Because honey bees produce secretions containing geraniol, citral, geranic and nerolic acids through the Nasonov glands, the use of these natural substances that are already present in the nest may not disturb the colony [ 46 , 47 , 48 ]. Moreover, many of these volatile and extractable compounds detected in the analyzed essential oils can be found in wax [ 49 ], honey [ 50 ] and in resins collected from plants and used to produce propolis by bees [ 51 , 52 ]. Therefore, it is plausible to expect an endogenous inhibitory action of all these compounds characteristic of the hive environment on this pathogen.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Activity of Several Essential Oils Extracted from Aromatic Plants against Ascosphaera apis

Pusceddu

Floris

Mangia

et al. 2021

Veterinary Sciences

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The use of natural substances such as essentials oils against bee pathogens is of great interest as an alternative to traditional methods based on synthetic compounds like antibiotics and fungicides, in order to minimize the risk of having toxic residues in hive products and to prevent the development of resistance phenomena. This study evaluated the inhibitory, fungicidal and sporicidal activity of ten essential oils extracted from aromatic plants against Ascosphaera apis, the etiological agent of chalkbrood, an invasive honey bee mycosis. The most effective essential oils were Thymus herba-barona, Thymus capitatus and Cinnamomum zeylanicum, which showed values of minimum fungicidal concentration and minimum sporicidal concentration ranging from 200 to 400 ppm. Carvacrol was the main component of Thymus capitatus and Thymus herba-barona oils, whereas cinnamic aldehyde prevailed in Cinnamomum zeylanicum oil. Further in-apiary studies will allow the evaluation of side effects on bees and residues in hive products.

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In vitro antibacterial activity and volatile characterisation of organic Apis mellifera ligustica (Spinola, 1906) beeswax ethanol extracts

Cited by 21 publications

References 60 publications

Biological Properties of Bee Bread Collected from Apiaries Located across Greece

Biological Properties of Bee Bread Collected from Apiaries Located across Greece

Antibacterial Activity of Honey Samples from Ukraine

In Vitro Activity of Several Essential Oils Extracted from Aromatic Plants against Ascosphaera apis

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