In vitro Antibacterial Activity and Phytochemical Analysis of Nicotiana tabacum L. Extracted in Different Organic Solvents

Abstract

Background:Controlling infectious disease using medicinal plants is the oldest healthcare known to mankind. Regardless of the enormous advances observed in modern medicine, medicinal plants are still playing vital roles. However, only a small proportion of medicinal plants are examined for bioactive compounds which may vary in different factors. This study aimed to evaluate phytochemical constituent and antimicrobial activities of Nicotiana tabacum L. extracted by different solvents against three set of bacteria.Methods: Nicotiana tabacum L. was collected from the Western Ethiopia and extracted in seven organic solvents. An in-vitro anti-bacterial activity of plant extracts was carried out by agar well diffusion assay against microbial type culture collection of human pathogens, clinical bacterial isolates, and biofilm forming bacteria. Gas Chromatographic and Mass Spectroscopic (GC-MS) analysis was used to determine the phytochemical constituents.Results:Antimicrobial activities of plant extract vary by extraction solvents; and ethyl acetate based extracts showed better antimicrobial activities. Of the experimental organisms, biofilm forming uropathogens were the most sensitive while clinical isolates were quite resistant. Analysis of the active ethyl acetate extract by GC-MS evinced a mixture of five volatile compounds; and Pyridine, 3-(1-methyl-2-pyrrolidinyl)-, (S) was the major compound detected. The overall results of the present study revealed that N. tabacum L extract has high antimicrobial activities against biofilm forming uropathogens.Conclusion:High antimicrobial activity was observed in ethyl acetate extract of N. tabacum against the biofilm forming bacteria whereas the clinically isolated bacteria were the most resistant group. The antibacterial property demonstrated could be due to Pyridine, 3-(1-methyl-2-pyrrolidinyl)-(S) with a broad spectrum of activity.

Antimicrobial Activity and Phytochemical Analysis of Some Selected Plants against Clinical Pathogens

“…23 In an earlier study, the methanol extract of the stem of Nicotiana tabacum effectively inhibited the growth of Pseudomonas aeruginosa. 24 The zone of inhibition of Nicotiana tabacum against Candida albicans was 50 mm, which concurs with the result of 25 where ethanol fraction was used. The range of activity of N. tabacum is broad, and its leaf extract has been used as a control agent against Plutella xylostella (Diamondback moth) and Grapholita molesta (Oriental fruit moth), which have proven resistant to pesticides in time past.…”

Ethnomedicinal Plants in Herbal Remedies Used for Treatment of Skin Diseases by Traditional Healers in Songkhla Province, Thailand

“…The most common phytochemical components found in the ethyl acetate extract were 3, 4, 5,6-tetrahydro-1, 3-dimethyl-2(1h)-pyrimidinone, pyridine, 3-(1-methyl-2-pyrrolidinyl)-, (S)-, isododecane, n -pentadecane, and tetradecylaldehyde. The antibacterial property demonstrated could be due to pyridine, 3-(1-methyl-2-pyrrolidinyl)-(S), the major compound detected, with a broad spectrum of activity [ 37 ]. Six sesquiterpenes, including tabasesquiterpenes A−C, balsamiferine B, samboginone, and ent-4(15)-eudesmen-1α,11-diol were isolated from the leaves of Nicotiana tabacum L. Tabasesquiterpenes B exhibited high antiviral activity with an inhibition rate of 35.2%.…”

Antibacterial Activity of Rosmarinus officinalis against Multidrug-Resistant Clinical Isolates and Meat-Borne Pathogens

“…is study is part of an ongoing screening program aiming at the discovery of Ethiopian medicinal plants with antimicrobial activities [24][25][26]. Despite the medicinal and extensive culinary uses of R. officinalis in different regions of Ethiopia, no previous studies have evaluated its antimicrobial activity against MDR clinical isolates as well as meat-borne bacterial pathogens.…”