In vitro antibacterial activities of compounds isolated from roots of Caylusea abyssinica

Edilu, Abdissa; Adane, Legesse; Woyessa, Delelegn

doi:10.1186/s12941-015-0072-6

Cited by 32 publications

(21 citation statements)

References 16 publications

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“…Such absorption frequency is identical to the absorption frequency of α-amyrin as stated by 14,15 -6). The chemical shift of the proton above is identical to the chemical shift of the hydrogen and carbon of βsitosterol 17,18 . The molecular structure of compound 2 similar to the molecular structure of β-sitosterol is supported by HSQC and HMBC spectrum data.…”

Section: Cells Viability (%) = Od Of Treated Cells Od Of Control Cellmentioning

confidence: 78%

Α–AMYRIN AND Β-Sitosterol FROM BARK EXTRACT OF RHIZOPHORA MUCRONATA LAMK. AND THEIR CYTOTOXIC ACTIVITIES AGAINST HELA CELL LINE

Nohong¹,

Soekamto²,

Ahmad³

et al. 2018

Int. Res. J. Pharm.

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The aims of this study are to isolate and identify active compounds of mangrove plants Rhizophora mucronata Lamk bark extract obtained from the mangrove forest of Kendari Bay, Southeast Sulawesi Province Indonesia and study their cytotoxic activities against cervical cancer (HeLa) cell line. The isolation and purification of the compounds were carried out under several chromatography methods, including thin layer chromatography, vacuum liquid chromatography and radial chromatography with silica gel as adsorbent and various solvents mixture as an eluent. Elucidation of the structure of the isolated compounds was done based on FTIR, 1 H-NMR, 13 C-NMR (1D and 2D) spectroscopic data and confirmed with an existed data from literatures. From this research, it was obtained two pure compounds, α-amyrin (1) and β-sitosterol (2). The isolation of this α-amyrin was firstly reported so far from the bark of this plant. Cytotoxic activities, marks by an IC50 value, of both α-amyrin and β-sitosterol are 804.762 ± 0.22 μg/mL and 353.871 ± 0.53 μg/mL respectively and shows that cytotoxic of β-sitosterol is stronger than α-amyrin.

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Section: Cells Viability (%) = Od Of Treated Cells Od Of Control Cellmentioning

confidence: 78%

Α–AMYRIN AND Β-Sitosterol FROM BARK EXTRACT OF RHIZOPHORA MUCRONATA LAMK. AND THEIR CYTOTOXIC ACTIVITIES AGAINST HELA CELL LINE

Nohong¹,

Soekamto²,

Ahmad³

et al. 2018

Int. Res. J. Pharm.

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“…35 Stigmasterol isolated from the methanolic extract of Aegle marmelos leaves shows anti-bacterial activity against S. aureus, E. coli, P. aeruginosa and S. thyphimurium when tested by zone inhibition assay. 36 Stigmasterol has an MIC of 100 µg/mL 37 and 1:1 mixture of stigmasterol and sitosterol has an MIC of 12.5 µg/mL against M. tuberculosis. 38 To our knowledge, this is the first study reporting the antitubercular activity of the hexane extract of the fruit of A. marmelos and we have identified six active principles in it which inhibited the growth of the virulent strain of M. tuberculosis.…”

Section: Discussionmentioning

confidence: 98%

Isolation and Characterization of Antimycobacterial Compounds from Fruits of Aegle marmelos (L.) Correa

Suja

Jose

Lakshmanan

et al. 2018

JCD

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Root extracts of Aegle marmelos, an evergreen tree indigenous to India, is an important ingredient in Ayurvedic preparations used for the treatment of pulmonary tuberculosis. Leaves of the tree are used as a remedy for respiratory ailments in folk medicine. The fruit is extensively used in the treatment of various bacterial diseases such as dysentery, diarrhea, etc. The present study was designed to screen organic solvent extracts of fruits of A. Marmelos for their inhibitory activity on Mycobacterium tuberculosis (M. tuberculosis), and to isolate and identify the compounds responsible for antimycobacterial activity. The minimum inhibitory concentration (MIC) of the extract and purified molecules on virulent laboratory strain M. tuberculosis H37Rv was determined by resazurin microtiter assay (REMA). Structural elucidation of the compounds was carried out employing NMR. Cytotoxicity of the compounds was evaluated against human monocyte THP1-derived macrophages by MTT assay. The hexane extract of A. marmelos fruits inhibited the growth of M. tuberculosis at an MIC of 50 µg/mL. Fractionation of the hexane extract by column chromatography led to the isolation of imperatorin, β-sitosterol, plumbagin, marmesin, marmin, and stigmasterol which individually inhibited the growth of M. tuberculosis. In vitro cytotoxicity assay revealed that the compounds are not cytotoxic at the concentrations that inhibited the growth of M. tuberculosis.

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“…Its chemical characteristics and structural properties are similar to that of (17-(5-ethyl-6-methylheptan-2-yl)-10,13-dimethyl-2, 3,4, 7,8 ,9,11, 12, 14,1 5,1 6,17 -dod eca hydr o-1Hcyclopenta[a]phenanthren-3-ol). β-sitosterol has been reported in literature [49][50][51][52] possessing antimicrobial activity from different plant species viz., Flemingia strobilifera, Dillenia indica, Momordica charantia, Caylusea absyssinica. However, the presence of this phytosterol in U. indica from Bastar possessing antifungal activity is the pioneer report of its kind.…”

Section: Discussionmentioning

confidence: 99%

Bioactive Compound in Urginea indica (Kunth.) from Bastar and its Spectral Analysis by HPLC, UV-Vis, FT-IR, NMR, and ESI-MS

Pandey

Gupta

2019

SN Compr. Clin. Med.

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The integration of traditional and modern medicine has gained increased recognition globally. In light of vast potentiality of medicinal plants as therapeutics, the present endeavor deals with the assessment of antifungal and synergistic activity of Urginea indica (Kunth.) (Family: Liliaceae) commonly known as wild onion against human pathogenic fungi viz., Aspergillu niger (MTCC 872) and Candida albicans (MTCC 183) procured from IMTECH, Chandigarh, India. The antifungal and synergistic activity was assessed by agar well diffusion method. The results were analyzed statistically using ANOVA with DMRT. The qualitative phytochemical analysis, column chromatography, TLC, MIC, and MFC was performed following standard protocols. Finally, the purified fraction was chemically characterized by HPLC, UV-Vis, FT-IR, (1 H & 13 C) NMR, and ESI-MS. The antifungal activity profile showed that acetone root extract exhibited broad-spectrum antifungal activity. However, in case of C. albicans, the extract exhibited significantly higher activity index. The qualitative analysis revealed the presence of varied phytochemicals. The extract exhibiting the highest activity was purified and tested for its synergistic or antagonistic potentiality against fungi revealed that the bioactive compound exhibited statistically significant synergism with three different commercially available antifungals viz., clotrimazole, ketoconazole, and fluconazole. Finally, the purified fraction was chemically characterized. The combination therapy against human pathogenic fungi seems to be a boon for patients with severe infections caused by multidrug-resistant pathogens. Thus, in current scenario, there is an urgent need to target new dimensions towards alternative antifungal therapy to preserve better human health for generations ahead.

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In vitro antibacterial activities of compounds isolated from roots of Caylusea abyssinica

Cited by 32 publications

References 16 publications

Α–AMYRIN AND Β-Sitosterol FROM BARK EXTRACT OF RHIZOPHORA MUCRONATA LAMK. AND THEIR CYTOTOXIC ACTIVITIES AGAINST HELA CELL LINE

Α–AMYRIN AND Β-Sitosterol FROM BARK EXTRACT OF RHIZOPHORA MUCRONATA LAMK. AND THEIR CYTOTOXIC ACTIVITIES AGAINST HELA CELL LINE

Isolation and Characterization of Antimycobacterial Compounds from Fruits of Aegle marmelos (L.) Correa

Bioactive Compound in Urginea indica (Kunth.) from Bastar and its Spectral Analysis by HPLC, UV-Vis, FT-IR, NMR, and ESI-MS

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