In vitro antibacterial activities of silver nanoparticles synthesised using the seed extracts of three varieties of Phoenix dactylifera

Abstract

Green synthesis of silver nanoparticles (AgNPs) is an ecofriendly, cost-effective and promising approach for discovery of novel therapeutics. The aim of the current work was to biogenic synthesize, characterize AgNPs using seed extracts of three economically important varieties of date palm (Iklas, Irziz and Shishi), and assess their anti-pathogenic bacterial activities. AgNPs were synthesised then characterised using electron microscopy and Fourier transform infrared analyses. The bactericidal activities of AgNPs against five different bacterial pathogens, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Streptococcus pneumoniae, were determined in vitro. In particular, changes in membrane integrity of virulent bacterial strains in response to AgNPs were investigated. Results of lactate dehydrogenase, alkaline phosphatase activity assays, and measurement of membrane potential revealed that the cytotoxic effects of the AgNPs were mainly centred on the plasma membrane of bacterial cells, leading to loss of its integrity and eventually cell death. In conclusion, green synthesis of AgNPs is an efficient, cost-effective and promising strategy to combat virulent antibiotic-resistant strains.

Síntese verde e caracterização de nanopartículas de prata AgNp estabilizadas em extrato de jurema preta (Mimosa tenuiflora)

“…As nanopartículas metálicas apresentam comportamento óptico observável em decorrência da forma e tamanho, isto proporciona absorções em regiões distintas do espectro visível denominadas bandas plasmons que é a Ressonância de Plasmons de Superfície (RPS). Quanto à estabilização e as dimensões das nanopartículas encontradas, os resultados convergem com estudos publicados sobre a formação de nanopartículas de prata e citrato (Aldayel et al, 2022).…”

Brassica oleracea L. (Acephala Group) based zinc oxide nanoparticles and their efficacy as antibacterial agent

“…Hence, synthesis of metal nanoparticles using green methods having antibacterial potential has become an alternative remedy for pathogens control. Various studies have reported that metal nanoparticles strongly inhibit the growth of pathogens due to disruption of plasma membrane which eventually leads to death of bacterial cells (Aldayel et al, 2022;Shah et al, 2021). Among metal nanoparticles of same nature, Zinc oxide nanoparticals are one of the most effective and safe antimicrobial agents which are being used as medicine as well as preservative in packaging since long (Baum et al, 2000).…”

Potential antibacterial and antioxidant inhibitory activities of Silybum marianum mediated biosynthesised He-Ne laser

“…S. typhi , E. coli ATCC 8739, and methicillin-resistant Staphylococcus aureus (MRSA) bacterial strains were acquired from King Faisal University’s College of Medicine. The disc diffusion assay was carried out in accordance with Aldayel et al, 2021 , Aldayel et al, 2022 , and Rana et al (2023) and nutrient broth was used for the cultivation of all microbial strains (Sigma Aldrich, Cat. no.…”