In vitro anti-Trichomonas vaginalis activity of Haplophyllum myrtifolium

Gökmen, Ayşegül Aksoy; Can, Hüseyin; Kayalar, Hüsniye; Pektaş, Bayram; Kaya, Selçuk

doi:10.3855/jidc.10854

Cited by 10 publications

(10 citation statements)

References 15 publications

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“…vaginalis and crude extract was more efficient. Gokmen et al studied efficacy of Haplophyllum myrtifolium on trichomoniasis (16). In the study, the efficacy of the plant was evaluated.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antitrichomonal Activity of Some Species of Allium

Majidi

Hezarjaribi

Arab

et al. 2019

Jundishapur J Nat Pharm Prod

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Background: Trichomonas vaginalis infectious disease is an important worldwide health problem. Although, several drugs especially metronidazole and tinidazole have been used in the treatment, their-resistant strains have been developed and unpleasant adverse effects exist. Garlic and other Allium species are old medicinal plants, which were used for infectious diseases, cardiovascular disorders and hair and skin cosmetic diseases. Objectives: We aimed to compare the anti-trichomoniasis activities of three species of Allium. Methods: The Trichomonas vaginalis strain was isolated from vaginal discharge of women with vaginitis symptoms and cultured in a modified TYM medium. After confirmation of herbarium of the plants, their extracts were prepared. For testing, 24 sterile plates were used. In all 24 homes, 200 µL of TYM were poured out. The tube containing the parasite, counting the number of angels with the aid of Neubauer slide and set to 500,000 per mL, was added to the amount of 100 µL to all wells. In order to investigate the antitrichomoniasis of the plant, concentrations of 37.5, 75, 150, 300, 600, 1200 and 2400 µg/mL of extract have been tested. For positive control, 50 mL of metronidazole, 50 µg/mL, was added to culture medium. In addition to, no herbal extract and metronidazole was added to the well of negative control. Results: The leaf extract of bell circus with a concentration of 2000 µg/mL after 48 hours and boiled cabbage bulb onion at a concentration of 24 µg/mL and leaf extract of turmericum at a concentration of 1500 µg/mL in 48 hours and boiled horseradish onions at 48 µg/mL concentration in 24 hours could achieve 100% GI and significantly inhibit parasite growth. Leaf extract, boiled onions and essential oils inhibited the growth of Trichomonas parasites, were not as high as 100%. Conclusions: According to the results obtained in our study, these Allium plants have inhibitory activity on growth of Trichomonas vaginalis parasite.

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“…vaginalis and crude extract was more efficient. Gokmen et al studied efficacy of Haplophyllum myrtifolium on trichomoniasis (16). In the study, the efficacy of the plant was evaluated.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antitrichomonal Activity of Some Species of Allium

Majidi

Hezarjaribi

Arab

et al. 2019

Jundishapur J Nat Pharm Prod

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“…Pteleine shows antitumor and antimicrobial activities [51]. Oxonantenine possesses cytotoxic activity [52] while gamma-Fagarine has antitrichomonas potential [53]. Erysopine has antifeedant potential [54].…”

Section: Uhplc-esi-qtof-ms Analysismentioning

confidence: 99%

Efficacy of Phytochemicals Derived from Roots of Rondeletia odorata as Antioxidant, Antiulcer, Diuretic, Skin Brightening and Hemolytic Agents—A Comprehensive Biochemical and In Silico Study

et al. 2022

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Roots of Rondeletia odorata are a rich source of phytochemicals with high antioxidant potential and thus may possess health benefits. This study used the LC-MS technique to identify phytoconstituents in R. odorata roots extract/fractions. Results revealed that n-butanol fraction and ethanolic extract contained total phenolic and flavonoid contents with values of 155.64 ± 0.66 mgGAE/g DE and 194.94 ± 0.98 mgQE/g DE, respectively. Significant potential of antioxidants was observed by DPPH, CUPRAC and FRAP methods while the ABTS method showed moderate antioxidant potential. Maximum % inhibition for urease, tyrosinase and carbonic anhydrase was shown by ethanolic extract (73.39 ± 1.11%), n-butanol soluble fraction (80.26 ± 1.59%) and ethyl acetate soluble fraction (76.50 ± 0.67%) which were comparable with thiourea (standard) (98.07 ± 0.74%), kojic acid (standard) (98.59 ± 0.92%) and acetazolamide (standard) (95.51 ± 1.29%), respectively, while all other extract/fractions showed moderate inhibition activity against these three enzymes. Hemolytic activity was also observed to range from 18.80 ± 0.42 to 3.48 ± 0.69% using the standard (triton X-100) method. In total, 28 and 20 compounds were identified tentatively by LC-MS analysis of ethanolic extract and n-butanol soluble fraction, respectively. Furthermore, molecular docking was undertaken for major compounds identified by LC-MS for determining binding affinity between enzymes (urease, tyrosinase and carbonic anhydrase) and ligands. It was concluded that active phytochemicals were present in roots of R. odorata with potential for multiple pharmacological applications and as a latent source of pharmaceutically important compounds. This should be further explored to isolate important constituents that could be used in treating different diseases.

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“…The ethanol extract, total alkaloid fraction, and pure compounds of Haplophyllum myrtifolium Boiss., a medicinal plant endemic in Turkey, were evaluated against T. vaginalis. Authors determined the MIC/MLC for each sample, resulting in 200/400, 400/800 and 50/150 µg/mL for ethanol extract, alkaloid extract, and skimmianine after 48 h of incubation, respectively [81]. Ethnopharmacological knowledge drove the trichomonacidal investigation of Asclepias curassavica L. However, the ethanolic extract of A. curassavica leaves and stem showed poor anti-T. vaginalis activity, with an IC 50 value of 302 µg/mL [68].…”

Section: Articles: Natural Productsmentioning

confidence: 99%

Novel Treatment Approaches to Combat Trichomoniasis, a Neglected and Sexually Transmitted Infection Caused by Trichomonas vaginalis: Translational Perspectives

et al. 2022

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The multistep translational science behind new drugs comprehends the entire process through laboratory, clinical, and community observations turned into health interventions. The development of new drug options from discovering targets and leading compounds in basic research for implementing therapeutic guidelines contributes to the emergence of health policies essential for infection control. This review updates the translational research in the scenario of the most common non-viral sexually transmitted infection (STI), trichomoniasis. Paradoxically to its high occurrence, it is considered neglected since notification is not mandatory. It turns into a stable disease with health complications, and receives little emphasis from public health programs to control STI. Although related to curable STIs, the current drugs, metronidazole and tinidazole, present therapeutic failures. The need for new options to treat trichomoniasis is established by basic research studies and patents revealing novel synthetic compounds and natural products presenting anti-Trichomonas vaginalis activities, mainly based on in vitro findings. Clinical trials are still focused on new routes of administration for conventional drugs. In addition, nanotechnology approaches are in their infancy, shedding light on potential possibilities for creating more effective, targeted, and safe delivery systems. Overall, the novel proposed approaches need, in addition to pharmaceutical development and efficacy assessments, to ensure that the quality requirements for their use as medicines are met. It is essential to overcome these issues to cross the “Death Valley” of drug discovery and to advance in the translational science criteria in the trichomoniasis drug development field.

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In vitro anti-Trichomonas vaginalis activity of Haplophyllum myrtifolium

Cited by 10 publications

References 15 publications

In Vitro Antitrichomonal Activity of Some Species of Allium

In Vitro Antitrichomonal Activity of Some Species of Allium

Efficacy of Phytochemicals Derived from Roots of Rondeletia odorata as Antioxidant, Antiulcer, Diuretic, Skin Brightening and Hemolytic Agents—A Comprehensive Biochemical and In Silico Study

Novel Treatment Approaches to Combat Trichomoniasis, a Neglected and Sexually Transmitted Infection Caused by Trichomonas vaginalis: Translational Perspectives

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