In-vitro anti-sickling and membrane stability potentials of Mishenland polyherbal extract on sickle red blood cells

Oyenike, Musiliu A.; Akpan, H; Otulana, Olatoye J; Adefule, A K; Adedokun, Kamoru A.; Oluogun, Waheed A; Muhibi, Musa Abidemi; Ojokuku, Hammed O.

doi:10.4103/ejh.ejh_33_18

Cited by 8 publications

(7 citation statements)

References 4 publications

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“…Previous studies have reported the presence of these phytochemicals in C. papaya fruit, stem, leaves, seeds, and bark (22,23). These compounds have been reported to contain the active principle for plants containing antisickling agents (24). Ibrahim et al (25) reported that antisickling activity of Hymenocardia acida Tul was found in the fractions containing flavonoids and saponin.…”

Section: Discussionmentioning

confidence: 95%

In-vitro antisickling and sickling-reversal activities of Carica papaya fruit at different stages of ripening

Adetayo¹,

Adetayo²,

Shokunbi³

et al. 2020

BUMJ

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Objective: Sickle cell disease (SCD) is a haemoglobinopathy that causes several clinical complications. Unripe Carica papaya has been shown to possess antisickling activity that could reduce these complications. This study aimed to examine the presence of antisickling and sickling-reversal activities of aqueous extracts and ethyl acetate fractions of C. papaya fruit at different stages of ripening. Methods: Unripe, partly ripe, and fully ripe fruits were quantitatively screened for some phytochemicals using standard methods. Blood samples from sickle cell patients were used to investigate antisickling and sickling reversal activities of aqueous extracts and ethyl acetate fractions of the fruits. Data were analysed using one-way ANOVA. The p-value was set at 0.05. Results: Phytochemicals such as alkaloids, saponins, tannins, flavonoids and polyphenols were found in varying concentrations in C. papaya fruit at the different stages of ripening. All extracts and fractions showed antisickling and sickling-reversal activities with the ethyl acetate fraction of partly ripe C. papaya being most effective. Conclusion: The study showed that C. papaya fruit at different stages of ripening contains antisickling and sickling-reversal activities which may help reduce the associated complications of SCD when consumed by affected individuals.

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Section: Discussionmentioning

confidence: 95%

In-vitro antisickling and sickling-reversal activities of Carica papaya fruit at different stages of ripening

Adetayo¹,

Adetayo²,

Shokunbi³

et al. 2020

BUMJ

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“…The inhibitory effects of bergapten on heat-treated proteins could be ascribed to the expansion and interaction with membrane proteins, an indication of antirheumatoid properties. Several clinical and experimental studies have shown that the pathophysiology of diseases such as malaria [ 42 ], diabetes [ 43 , 44 ], artherosclerosis [ 45 ], sickle cell anaemia [ 46 ], and arthritis [ 15 , 16 ] among others involve erythrocyte membrane instability coupled with protein denaturation. As such, the protective effects of bergapten against erythrocyte membrane destruction and protein denaturation could contribute to the amelioration of such diseases.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Bergapten against Human Erythrocyte Hemolysis and Protein Denaturation In Vitro

Aidoo

Konja

Henneh

et al. 2021

International Journal of Inflammation

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Bergapten, a furocoumarin found in many medicinal plants, is used for the management of various conditions. The present in vitro study evaluated the ability of bergapten to prevent human erythrocyte hemolysis and protein denaturation. Bergapten administered at 10, 30, and 100 μg/ml exhibited a significant concentration-dependent protection on the erythrocyte membrane exposed to hypotonicity and heat-induced hemolysis. The concentration at which bergapten inhibited 50% of the cells from hemolysis (IC50) was determined on a dose-response curve, plotted as logarithmic (concentration) against percentage inhibition, keeping the hemolysis produced within the control group at 100%. Bergapten treatment produced an IC50 value of 7.71 ± 0.27 μg/ml and 4.23 ± 0.42 μg/ml for hypotonicity and heat-induced hemolysis, respectively. Diclofenac sodium at similar concentrations produced an IC50 value of 12.22 ± 0.30 μg/ml and 9.44 ± 0.23 μg/ml in the hypotonicity and heat-induced hemolysis, respectively. The ability of bergapten to inhibit protein denaturation was studied as part of an investigation on its mechanism of action. The results showed a significant concentration-dependent reduction in protein denaturation. When administered at 10, 30, and 100 μg/ml, bergapten produced a concentration-dependent reduction in albumin denaturation. Bergapten inhibited protein denaturation with IC50 values of 5.34 ± 0.30 μg/ml and 12.18 ± 0.20 μg/ml in the heat-treated egg albumin and bovine serum albumin denaturation experiments, respectively. Diclofenac sodium (10, 30, and 100 μg/ml) exhibited a similar protection against heat-treated egg albumin and bovine serum albumin denaturation experiments with IC50 values of 8.93 ± 0.17 μg/ml and 12.72 ± 0.11 μg/ml, respectively. Taken together, data from this study show that the pharmacological properties of bergapten may in part be related to its membrane-stabilizing and antidenaturation properties.

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“…Images were obtained using the microscope (Nikon Eclipse TE2000-S) & smartphone setup and labeled as "Before treatment." Sodium metabisulfite (2% solution) was used to deoxygenate the RBCs (0.2 g of sodium metabisulfite in 10 ml of nano-pure water) [4,28]. Sodium metabisulfite is a reducing agent which promotes sickling [29,30].…”

Section: Sample Preparationmentioning

confidence: 99%

“…Sickle cell disease (SCD) is a common worldwide genetic disorder caused by the single point mutation in the beta-globin gene [1][2][3]. The β-6 glutamic acid is substituted by Valine, leading to the transformation of normal hemoglobin into HbS [4]. At low levels of oxygen, the HbS polymerizes, and results in sickled shape RBCs [5,6].…”

mentioning

confidence: 99%

Smartphone-based sickle cell disease detection and monitoring for point-of-care settings

Ilyas

Sher

et al. 2020

Biosensors and Bioelectronics

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Sickle cell disease (SCD) is a worldwide hematological disorder causing painful episodes, anemia, organ damage, stroke, and even deaths. It is more common in sub-Saharan Africa and other resource-limited countries. Conventional laboratory-based diagnostic methods for SCD are time-consuming, complex, and cannot be performed at point-of-care (POC) and home settings. Optical microscope-based classification and counting demands a significant amount of time, extensive setup, and cost along with the skilled human labor to distinguish the normal red blood cells (RBCs) from sickled cells. There is an unmet need to develop a POC and home-based test to diagnose and monitor SCD and reduce mortality in resource-limited settings. An early-stage and timely diagnosis of SCD can help in the effective management of the disease. In this article, we utilized a smartphone-based image acquisition method for capturing RBC images from the SCD patients in normoxia and hypoxia conditions. A computer algorithm is developed to differentiate RBCs from the patient's blood before and after cell sickling. Using the developed smartphone-based technique, we obtained similar percentage of sickle cells in blood samples as analyzed by conventional method (standard microscope). The developed method of testing demonstrates the potential utility of the smartphone-based test for reducing the overall cost of screening and management for SCD, thus increasing the practicality of smartphone-based screening technique for SCD in low-resource settings. Our setup does not require any special storage requirements and is particularly useful in assessing the severity of the SCD. This is the characteristic advantage of our technique as compared to other hemoglobin-based POC diagnostic techniques.

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In-vitro anti-sickling and membrane stability potentials of Mishenland polyherbal extract on sickle red blood cells

Cited by 8 publications

References 4 publications

In-vitro antisickling and sickling-reversal activities of Carica papaya fruit at different stages of ripening

In-vitro antisickling and sickling-reversal activities of Carica papaya fruit at different stages of ripening

Protective Effect of Bergapten against Human Erythrocyte Hemolysis and Protein Denaturation In Vitro

Smartphone-based sickle cell disease detection and monitoring for point-of-care settings

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